52results about How to "Easy to observe growth" patented technology

The present invention discloses a method for culturing Collybia albuminosan which is symbiotic with white ants; the white ant is artificially propagated and the propagated white ants are moved into a formicary; the depth of the formicary is 60 cm with a diameter of 40 cm; the feed for white ant is placed at the bottom of the formicary; after the white ants are moved into the formicary, an 25 to 30 deep earth layer is covered on the formicary; the Collybia albuminosan seeds are sown in the earth. Moreover, corn, peanut or fruit tree can be planted on the earth. The feed of white ant is formed by fermentation of nine powder materials of Cyclobalanopsis glauca, dry walnut tree, corn cob core, bean smash powder, iron-like silky oak, soaking silky oak, corn straw, sorghum straw and sorghum rice and water. In this way, an excellent growing and propagating environment is established for white ants and conditions for Collybia albuminosan and other crops are created; a plurality of matched food chains are developed in a solid way which increase s the use value of the land.

The invention provides meat duck stacked automatic breeding equipment which comprises cage shelf units. Each cage shelf unit comprises a head end, a tail end and a meat duck framework, wherein the head end and the tail end are arranged at the two ends of the meat duck framework respectively; meat duck cages are arranged on the meat duck framework in a stacked mode, and the meat duck framework is also provided with food troughs corresponding to the meat duck cages. Each meat duck cage comprises a bottom net, a front net, a partition net on the left side, a partition net on the right side and a detachable rear door. The two sides of each cage shelf unit are provided with channels, wherein the channel on the food trough side is a management channel, and the channel on the side opposite to the food trough is a duck-out channel. The invention further provides a meat duck cage breeding management method, wherein the go-out mode is that ducks go out of the rear sides of the cages. The meat duck stacked automatic breeding equipment is simple in structure, meat ducks raised according to the management method are high in quality and low in disability rate, and economic benefits of farmers are greatly increased.

The invention belongs to technical field of animal genetic engineering, and specifically relates to immunodeficient mice, a manufacturing method thereof and applications. The manufacturing method comprises using NOD-Scid IL2rg- / - immunodeficient mice (NSI mice), and deleting Foxnl gene or Fah gene. Through deleting the Fah gene on the NSI mice, NSIF mice are obtained. The NSIF mice can be used to efficiently establish a novel humanized mouse model, used for liver physiological and pathological research. Through deleting the Foxnl gene on the NSI mice, the NSIF mice are obtained. The NSIF mice have no body hair, and immune system is further defected. The NSIF mice provide convenience for establishment, monitoring, and measurement of solid tumor.

The invention discloses a silver and black composite film and white film spaced mulch. The mulch comprises silver layer films, black layer films and white films and is characterized in that the silver layer films and the black layer films are composited to form silver and black composite film segments; the silver and black composite film segments and the white films are spaced; the adjacent edges of the silver and black composite film segments and the white films are combined into a whole via V grooves. The mulch has the beneficial effects that the silver layer films and the black layer films are composited and are spaced by the white films and the spacing distance can be selected according to the growth requirements of different crops, so not only can light be reflected via silver gray or white and heat be absorbed by black but also the ranges of application of the crops can not be reduced and the functions of preserving moisture, preserving heat, improving the soil properties, increasing the nutrient utilization rate, changing the growing period, controlling weeds and avoiding insect pests are further improved comprehensively; by taking low-price linear low density polyethylene as a main material and increasing the proportion of a silver gray masterbatch, the mulch is low in production cost, has good reflective rate and strong aging resistance and is beneficial to application in south China.

The invention discloses a simple seedling raising device for Arabidopsis thaliana, which comprises an annular upper floating plate (1) and an annular lower floating plate (3). The annular upper floating plate (1) is located above the annular lower floating plate (3), and the annular upper floating plate (1) ) is equal to the inner diameter of the annular lower floating plate (3), a planar mesh (2) is set between the annular upper floating plate (1) and the annular lower floating plate (3), and the outer diameter of the mesh (2) is larger than the annular The inner diameter of the upper floating plate (1). The present invention also discloses a simple method for growing seedlings of Arabidopsis thaliana by using the above-mentioned device, which includes the following steps: 1) pre-disinfection treatment; 2) spreading the above-mentioned disinfected Arabidopsis seeds evenly to the above-mentioned disinfected On the mesh (2) of the simple seedling raising device for Arabidopsis thaliana seeds; 3) make the simple seedling raising device for Arabidopsis thaliana seeds obtained in step 2) float and cultivate in the container (4) with 1 / 2 full Hoagland nutrient solution inside .

The invention relates to the technical field of biology, particularly a microalgae culture reactor capable of setting different concentration CO2 culture environments, which comprises a culture box, an ultraviolet lamp tube, a gas inlet pipe and a gas outlet pipe, wherein the box body of the culture box is a transparent box body; the lower side of the front end of culture box is provided with a gas inlet; the upper side of the back end of the culture box is provided with a gas outlet; the gas inlet pipe and gas outlet pipe respectively connected with the gas inlet and gas outlet of the culture box are respectively provided with a gas flowmeter and a valve; a CO2 gas analyzer for detecting carbon dioxide concentration at the gas inlet and gas outlet is arranged outside the culture box; the gas inlet and gas outlet of the culture box are respectively provided with a gas filter unit; and a humidification system is arranged in the culture box. A plurality of culture box can be arranged in parallel. By setting different concentration CO2 culture environments to screen the wild type microalgae or corresponding mutants, the complete reactor is simple in structure and convenient to operate.

The invention relates to a circularly holding type pesticide applying unmanned aerial vehicle for high-crown thinning. The unmanned aerial vehicle comprises a rack, a power and control mechanism, an atomizing operation executing mechanism and a suspension hook fastening device, wherein the power and control mechanism is used for providing fly power through rotor wings, the atomizing operation execution mechanism is installed on the rack, the suspension hook fastening device is used for fastening the unmanned aerial vehicle on twigs when the rotor wings cease to rotate, the suspension hook fastening device is installed on the rack, and the power and control mechanism comprises a flight control module used for controlling. The invention further relates to a pesticide applying method of the circularly holding type pesticide applying unmanned aerial vehicle for high-crown thinning. According to the unmanned aerial vehicle, the unmanned aerial vehicle is hung on to-be-operated twigs to perform operation, after the unmanned aerial vehicle reaches a certain height, the unmanned aerial vehicle is fastened on the to-be-operated twigs, the unmanned aerial vehicle has the advantages of energysaving, and the unmanned aerial vehicle belongs to the technical field of forestry machinery equipment.

The invention discloses a novel environment-friendly and energy-saving growth method of large-sized blocky information storage ferroelectric single crystals. The method comprises the following steps: heating an information storage ferroelectric single crystal raw material solution to realize the saturation equilibrium; seeding; transforming; carrying out programmed freezing growth; and performing post-treatment and heat crystal transferring. A single crystal mother liquid bottle does not need to be protected by protective gas and internal and external pressure balance can be controlled automatically in a liquid sealing way by using paraffin oil; air circulation between inside and outside is isolated by the paraffin oil and the temperature balance can be maintained precisely. According the method, the large-sized blocky information storage ferroelectric single crystals grow out indirectly; the manufacturing procedure is simple, the raw materials are simple and cheap, waste liquid is recyclable, no pollution or emission of the three wastes is caused; energy is saved since the crystals grow almost at room temperature; environmental friendliness is realized by virtue of growth in an aqueous solution; the manufacturing cost is low.

The invention discloses a method for prolonging the subculturing interval time of tissue culture seedlings of apple. A culture medium comprises the following components: 0.5-0.75 mg / L of MS+BA, 0.05 mg / L of NAA, 40.0 g / L of white granulated sugar and 6.0 g / L of agar; the packing quantity is 40-50% more than that in conventional breeding, and a bottleneck is sealed by using a plastic sealing film; tissue culture seedlings of apple are transferred to the culture medium and slowly grow about 2 weeks under the culture conditions that the temperature is 22-28 DEG C, the illumination intensity is 2000 lx, and the illumination time is 10-14 h / d, and then the tissue culture seedlings of apple are placed in a chemical agent storage box with a temperature of 2-8 DEG C so that the growth is slowed down; and when the culture medium is approximately dried, the tissue culture seedlings of apple are taken out of the refrigerated container to grow normally for 1-2 weeks or are directly transferred to a new culture medium. In such a way, the subculturing interval time of tissue culture seedlings of apple can be successfully prolonged from 2-3 months to 15-18 months, the survival rate of stem tips is more than 90%, the workload of subinoculation is greatly reduced, and the mutation probability is also reduced. The technique is applied to the germplasm preservation of plantlets of apples and other parts of orchardfruits and flowers, is simple, convenient, saving, and high in efficiency, and provides a new way for the preservation of germplasm resources.

This invention relates to a growing method for a flux of a non-linearity optical crystal including preparing materials according to the mol ration of 1;5-7.54 : 2.3-3.5: 8.75-35 of Na3La9B8027:Na20:B203 :NaF pre-processing, then rising the temperature in a crystal growing furnace till the complete melt, in which, the constant temperature uniforms the solution fully then introducing inoculating crystals above the saturation temperature of 5-20deg.C and keeping constant for 10-30mitutes then reducing the temperature quickly to the saturated, controlling the parameters to grow crystals, then the crystals break away from the surface to reduce the temperature to the room in the rate not greater than 50deg.C / h to get the crystal.

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

The invention relates to a growth method for large-size information storage monocrystals. The growth method is simple, ingenious, energy-saving and environment-friendly, mild conditions with the temperature below 50 DEG C are required only, alcoholic-solution growth is non-toxic, and waste liquid is recycled. During phase transition, the transition of a non-ferroelectric large-size monocrystal I crystal form to a required large-size monocrystal II crystal form can be completed by only maintaining the temperature of 168 DEG C for a few minutes. Meanwhile, the most complicated crystal transition step is avoided, and seed crystals are directly hung in mother liquor, so that the operation is simpler, the equipment is simpler and easier, the cooling rate is higher, and the growth cycle is shortened. The aftertreatment method is improved to be simpler, and crystals are directly drawn out and put into a synthermal constant-temperature oil bath. The quality of the improved crystals is not affected.

The invention relates to a fast check reagent of Salmonella causing neutralization food source type food poisoning, in particular to a fast diagnosis reagent for a patient with most common Salmonella causing food source type food poisoning after a plurality of antibiotics are clinically used and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum and a neutralizing agent are added in enriched liquid, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the neutralizing agent can neutralize a plurality of residual antibiotics or disinfectors contained in a specimen and generate a neutralized product having no influence on the growth of Salmonella. The identification accuracy is larger than 99 percent, the fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria; and the check diagnosis process is shortened to 2-6 hours.

The invention discloses a method for studying striga asiatica lactone in regulating rape root system growth. The method comprises the steps that 1, the rape seeds are soaked into ethyl alcohol and rinsed with distilled water; 2, the seeds are evenly put on a culture dish of wet filter paper for germination accelerating under illumination in a constant-temperature growth box; 3, a plastic cup of which the diameter is filled with the distilled water is taken, a cup opening is covered with gauze, the gauze is soaked into water, rapeseeds after germination accelerating are put on the gauze, and rape seedlings with neat growth are fixed to a cystosepiment, and are cultured in a 1 / 2 Hoagland's nutrient solution; 4, the rapes are moved to striga asiatica lactone solutions with different concentrations for growth, and are sampled every day using a root system scanner to be scanned; 5, rape groups are treated, and materials which are extremely sensitive and insensitive to striga asiatica lactone are screened out. The method for studying striga asiatica lactone in regulating the rape root system growth is feasible and convenient to operate, better facilitates research of shapes of plants, is harmless to root systems, screens the materials of which the root system growth has different sensitivity to striga asiatica lactone, and lays a foundation for later physiology and biochemistry, molecular biology and thremmatology research.

The invention discloses a crayfish breeding, hatching and collecting device. The device comprises a culture pond, a circulating water tank and a sewage tank, the outer side of a bottom plate of the culture pond is arranged in the circulating water tank through supporting legs, the sewage tank is arranged on one side of the circulating water tank, a circulating water pump is arranged on the other side of the circulating water tank, one end of the circulating water pump is in communication with the culture pond through a circulating water inlet pipe, and the other end of the circulating water pump is in communication with the circulating water tank through the circulating water inlet pipe. According to the crayfish breeding, hatching and collecting device, a cleaning brush is used for cleaning the bottom of the culture pond, residues of impurities at the bottom of the culture pond are reduced, and the culture pond is convenient to clean; meanwhile, a sewage outlet valve is opened, impurities flow out from a sewage outlet pipe and enter the sewage tank, the circulating water pump can enable water in the culture pond and the circulating water tank to circulate, the activity of the water is kept, and therefore, the activity of juvenile crayfish is improved; and the crayfish breeding, hatching and collecting device is reasonable in structure, convenient to use and suitable for popularization and application.

The invention discloses a high-throughput preparation method of multicellular tumor spheroids. The preparation method comprises the following steps: S1, quickly mixing a cell suspension of tumor cellswith a basement membrane matrix, and transferring the obtained cell suspension into a low-attachment well plate; and S2, performing centrifuging, culturing the cell suspension in an incubator after centrifugation, and maintaining the cell suspension during culture to form the multicellular tumor spheroids combined with the basement membrane matrix. The added substance used in the technical schemeis the basement membrane matrix Matrigel which can promote the formation and growth of the multicellular tumor spheroids, thereby promoting the three-dimensional structure of the formed multicellulartumor spheroids; meanwhile, the low-attachment well plate is used, so the tumor cells spontaneously aggregate into spheroids; and combined with culture after centrifugation of the well plate and thedesign of the step of maintaining the cell suspension during culture, cell aggregation is artificially promoted by centrifugation, so the subsequently formed cell spheroids are more compact and have amore three-dimensional structure. Therefore, the prepared tumor spheroids are in a state of uniform diameter and concentrated particle size distribution.

The invention provides a pig raising house with a monitoring system. An air conditioner is arranged in the pig raisin house; an arch bottom surface is arranged in the pig raising house; a closed heat exchange tank is arranged at the bottom of the pig raising house; a heat exchange tube is arranged in the heat exchange tank; a steam outlet and a steam inlet of a solar thermal collector are connected with the two ends of the heat exchange tube respectively; an air conditioning device is further arranged on the heat exchange tube; and a camera is arranged at each of four corners of the top part of the pig raising house and aims at the center point of the inner space of the pig raising house. The arch bottom surface of the pig raising house ensures that peculiar smells in the pig raising house are reduced, and the environment in the pig raising house is kept; the heat exchange tank, which is supplied with heat by the solar thermal collector and is arranged at the bottom of the pig raising house, is used for supplying heat to the inside of the pig raising house; the air conditioner arranged in the pig raising house is mainly used for cooling the pig raising house in summer; the breeding cost is reduced; and the monitoring cameras arranged in the pig raising house are convenient to observe the environment in the pig raising house and the growing states of pigs.

The invention discloses a soilless vegetable culture greenhouse water economizer. The soilless vegetable culture greenhouse water economizer comprises a greenhouse body, the bottom of the greenhouse body is provided with a greenhouse foundation which is arranged on both sides of the top of a concrete layer, and the bottom of the concrete layer is provided with a thermal insulating layer; the innertop of the greenhouse body is provided with a steel structure triangular fixing frame, the bottom of the steel structure triangular fixing frame is provided with a beam and a plurality of support plates, wherein the beam is arranged in the middle of the bottom of the steel structure triangular fixing frame, the support plates are uniformly arranged on both sides of the beam, the bottom of the beam is provided with a sliding rail which is matched with a slider, and the bottom of the slider is provided with a hanging loop; corresponding culture stands are arranged between the support plates andthe concrete layer, and every culture stand is sequentially provided with a culture groove I, a culture groove II and a culture groove III, and a water spray device I is arranged above the culture groove I. The soilless vegetable culture greenhouse water economizer has the advantage of being high in efficiency and saving labor and material resources.

The invention provides a plant aeroponic culture control device and method. The Plant aeroponic culture control device comprises an atomization control box, an aeroponic culture box, image pickup equipment and a computer; the atomization control box is provided with a controller; the aeroponic culture box is connected with the atomization control box; the image pickup equipment is used for acquiring an image of a plant; the computer is respectively connected with the controller and the image pickup equipment; the computer is used for acquiring sectional areas and minimum enclosing rectangle parameters of a leaf crown and a root of a plant according to the image of the plant, acquired by the image pickup equipment, computing the sectional area ratio of the leaf crown to the root and the area ratio of minimum enclosing rectangles and sending a computed result to the controller; and the mist delivery quantity of the atomization control box is controlled by the controller. The plant aeroponic culture atomization control based on a root shoot ratio is particularly suitable for aeroponic culture atomization control leaf vegetables, not only can the traditional aeroponic culture operationbe finished, but also a corresponding regulation strategy can be computed according to the growth condition of the vegetables, the condition that the leaf vegetables are excessively developed in roots and slightly few in leaves in an aeroponic culture process is solved, and the problem of growth vigor of roots and crowns is controlled to a certain extent.

The present invention discloses an artificial cultivation method for stone-like omphalia. The artificial cultivation method specifically comprises: breeder seed preparation, cultivated seed preparation and indoor or outdoor soil covered cultivation. The method has the advantages that: corn is used as a main raw material for preparing breeder seeds of stone-like omphalia, and the problem that hyphae grow slowly in the breeder seed cultivation process is solved; Oak sawdust is used as a main material, soybean meal, wheat bran and corn flour are used as auxiliary materials, and KH2PO4, gypsum and the like are used as mineral elements in the the cultivation, so that the growth speed of stone-like omphalia hyphae is accelerated, and a production cycle is greatly shortened; corn straws and bamao straws are used in indoor and outdoor cultivation, and are cheap and easy to obtained, so that the cost is low, wooden material needs are reduced, and protection of the ecological environment is provided; and stone-like omphalia sclerotium is obtained by a soil covered cultivation technology, the operation is simple and easy, the yield is high, the labor cost is reduced, and the economic benefits of the stone-like omphalia production are increased.

The invention relates to a purity evaluation device for electronic-grade trichlorosilane. The device comprises a chassis, a bell-jar, which is arranged on the chassis and has a cooling water jacket, a tantalum filament, which is arranged in the bell-jar with the cooling water jacket, and a pair of electrodes, which go through the chassis and are connected to the power supply. The top part of the bell-jar with the cooling water jacket is provided with a hanger and a bell-jar cooling water outlet, the side wall of the bell-jar with the cooling water jacket is provided with a bell-jar cooling water inlet and a sight glass; the two electrodes are connected through the tantalum filament, the outside of the pair of electrodes is provided with a reaction gas inlet and a reaction gas outlet, and the reaction gas inlet and the reaction gas outlet go through the chassis and communicate with the cavity of the bell-jar with the cooling water jacket. The purity evaluation device for electronic-grade trichlorosilane has the advantages of simple structure and low cost, achieves the goals of rapid and precise purity measurement, and realizes safety operation.

The invention provides a novel edible mushroom culturing bag. The novel edible mushroom culturing bag comprises an inner bag and an outer bag, wherein the inner bag is made of transparent plastic; the inner bag is oppositely folded along the axis and sealed at the bottom, and a sealing line is a straight line; a culture medium is arranged inside the inner bag; a circular hole is formed at a central axis of the culture medium; a bag mouth of the inner bag is folded into the circular hole; a circular mushroom hole stick matched with the circular hole in diameter is inserted into the circular hole; the outer bag is made of black heat-shrinkable plastic; the outer bag sleeves the part, filled with the culture medium, of the inner bag. The novel edible mushroom culturing bag is reasonable in design and simple in structure; the inner bag is oppositely folded along the axis and sealed at the bottom, so that the sealing performance is good, the bearing capability of the inner bag can be enhanced and the inner bag is unlikely to break; the inner bag is made of transparent plastic, so that mycelial growth can be observed conveniently; the outer bag is made of black heat-shrinkable plastic so as to avoid light irradiation of mushrooms in the later growth stage, so that can the aging rate of the mushrooms can be delayed and bacteria extend the shelf life of the strain can be prolonged.

The invention provides a potting barrel for analyzing influence of warming on the distribution state of wheat roots. The potting barrel with an open upper part is provided with a plurality of cross-cut openings and sieve pore supporting plates used for growth positioning of the wheat roots along a height direction; the plurality of the cross-cut openings are arranged on the side wall of the potting barrel at intervals; the sieve pore supporting plates are arranged at the inner side of the potting barrel in an upper-lower interval manner, so that a substrate in the barrel is divided into a plurality of layers of wheat root growth space independent to one another; and each layer of the circumferential side wall in the potting barrel is provided with one spiral waterway backflow heating tubeunit. According to the potting barrel provided by the invention, the wheat roots in a required soil layer are intercepted in a targeted manner, so that the wheat roots are more convenient and quickerto cut, integrity of the whole soil layer is guaranteed, the growth condition of the wheat roots is convenient to observe, the growing environment and soil temperature of plants to be tested can be accurately controlled, spatial distribution of the wheat roots can be measured in situ, accuracy of test data can be improved, and the purposes of spatial intensification and operation convenience can be achieved.

The invention relates to a water-culture planting device. The water-culture planting device comprises a culturing pool and a liquid storage pool which are arranged from top to bottom. The water-culture planting device is characterized in that the side wall of the culturing pool is provided with a plurality of fixed planting holes and overflowing through holes which are communicated with the inner wall and the outer wall of the side wall of the culturing pool; the plurality of fixed planting holes and overflowing through holes are respectively formed by equal height, and the height of each overflowing through hole is lower than that of each fixed planting hole; the side wall of the liquid storage pool extends outwards in a manner of being relative to the side wall of the culturing pool, and an opening of the extending part forms an opening part; the opening part is provided with an oblique and detachable flow-guiding insulated cover plate; the side wall, which corresponds to the extending part, of the liquid storage pool is obliquely arranged in an outwards-opening manner; a flow-guiding gap is formed between the inclined bottom part of the flow-guiding insulated cover plate and the edge of the opening part. The water-culture planting device brings convenience for realizing multi-layer industrial planting, and has the advantages that the structure is simple and the growth condition of plants can be conveniently observed.

The invention belongs to the technical field of culturing frames, and particularly provides an authenticating frame and a rice seedling stage rhizoctonia solani authenticating device comprising the authenticating frame. The authenticating frame comprises a square frame body with supporting feet and partition plates; a connecting rod is arranged on each of the two sides of the frame body; a supporting rod is arranged on the side surface, adjacent to each connecting rod, on the frame body; each supporting rod is provided with through holes and supporting inserting blocks which are matched with the through holes; the middle parts of the two sides of each partition plate are hinged to the frame body through the connecting rods; one end of each partition plate is erected on the corresponding supporting inserting block; protruded partition strips are arranged on each partition plate at intervals and are parallel to the connecting rods; a film rod extending out of the frame body is also arranged above each supporting foot on the frame body; each film rod is provided with a first film rod and a second film which are respectively parallel to adjacent side surfaces of the frame body. The partition plates of the authenticating frame are adjustable so as to facilitate the observation of crop growth situations inside the authenticating frame; the arrangement of the structures, such as the film rods, is convenient for covering the authenticating frame with a thin film and facilitates the adjustment of the temperature and the humidity in the authenticating frame.

The invention discloses a culturing device for culturing edible mushroom. The culturing device comprises an outer cylinder, an inner cylinder, a carbon dioxide absorption device and a fixing bolt, wherein the outer cylinder is provided with a base plate and an upper cover which are both detachably installed on the outer cylinder; the inner cylinder is placed in the outer cylinder, and is lower than the same; the carbon dioxide absorption device is fixed on the inner wall of the upper end of the outer cylinder, and a viewing port is formed in the side wall, corresponding to the upper side of the inner cylinder, of the outer cylinder; the fixing bolt passes through the side wall of the outer cylinder and is pressed against the outer wall of the inner cylinder for connecting and fixing the outer cylinder with the inner cylinder; a flange radially protruding outwards is arranged at the outer end of the inner cylinder, and the fixing bolt is tightly fit with the top surface of the flange. According to the culturing device, the inner cylinder is convenient to put in and take out, and utilization and operation are facilitated; the outer cylinder and the inner cylinder are fixed by the fixing bolt and the flange arranged at the lower end of the inner cylinder, so that structural stability is good, and utilization and transportation are facilitated; furthermore, a growing condition of agrocybe cylindracea can be conveniently observed via the viewing port, thus being beneficial to ensuing culturing quality of the agrocybe cylindracea.