Simple seedling raising method of Arabidopsis thaliana and the device used

Abstract

The invention discloses a simple seedling raising device for Arabidopsis thaliana, which comprises an annular upper floating plate (1) and an annular lower floating plate (3). The annular upper floating plate (1) is located above the annular lower floating plate (3), and the annular upper floating plate (1) ) is equal to the inner diameter of the annular lower floating plate (3), a planar mesh (2) is set between the annular upper floating plate (1) and the annular lower floating plate (3), and the outer diameter of the mesh (2) is larger than the annular The inner diameter of the upper floating plate (1). The present invention also discloses a simple method for growing seedlings of Arabidopsis thaliana by using the above-mentioned device, which includes the following steps: 1) pre-disinfection treatment; 2) spreading the above-mentioned disinfected Arabidopsis seeds evenly to the above-mentioned disinfected On the mesh (2) of the simple seedling raising device for Arabidopsis thaliana seeds; 3) make the simple seedling raising device for Arabidopsis thaliana seeds obtained in step 2) float and cultivate in the container (4) with 1 / 2 full Hoagland nutrient solution inside .

Description

technical field [0001] The invention relates to a simple seedling raising method of Arabidopsis thaliana seeds and a device used therefor. Background technique [0002] In the past 20 years, Arabidopsis has been widely used as a model plant in plant life science research; the 10-year whole genome sequencing work was completed in 2000. Although the genome is small, the more than 25,000 genes of Arabidopsis are roughly similar to other flowering plants in terms of functional categories, so they are widely used as experimental materials for gene cloning and establishment of saturated mutant libraries. Using Arabidopsis thaliana to carry out botany research can mine the information of various metabolic pathways in plants, so it is also widely used in the research and exploration of the mysteries of plant life. [0003] At present, the plant functional genomics research using Arabidopsis thaliana is in full swing at home and abroad, so the correct and convenient seedling raising...

AI Technical Summary

Problems solved by technology

But there are certain deficiencies in these methods, and its biggest shortcoming is that seedlings are easy to infect bacteria, dry up, and it is unfavorable for the transplanting of seedlings and the observation of root system growth status, etc.

Images