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Artificial cultivation method for stone-like omphalia

A technology of artificial cultivation and thunder pills, applied in the field of microorganisms, can solve problems such as slow growth of mycelium, and achieve the effects of accelerating growth speed, shortening production cycle and improving economic benefits.

Active Publication Date: 2015-12-23
重庆市药物种植研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the object of the present invention is to provide a method for artificial cultivation of Lei Wan. The method of the present invention solves the problem of slow mycelium growth in the original seed cultivation process, improves the artificial planting output of Lei Wan, and reduces production costs. , which is conducive to the promotion of artificial cultivation of Leiwan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Original seed preparation

[0039] a. Preparation of stock matrix

[0040] Choose corn kernels that are free from mildew and rot, add water to boil until the endosperm has a line of white heart, filter, and add 2% gypsum and 0.1% MgSO according to the mass percentage of corn kernels 4 , 0.2% KH 2 PO 4 , stir and mix well, put it into a 650mL seed bottle, sterilize at 121°C and 103-115kPa for 1.5h, and cool to room temperature.

[0041] b. Vaccination

[0042] Inoculation needs to be performed on a clean bench. Before inoculation, the inoculation room should be sterilized by ultraviolet light. First, use 75% alcohol to sterilize the hands and inoculation needles. The inoculation needles, test tube ports and tampons must be sterilized with the flame of an alcohol lamp. Select strains that grow vigorously, have a relatively short age, and have no pigment secretions in the mycelium layer for inoculation, and use an inoculation needle to pick up 1 cm in the mother s...

Embodiment 2

[0057] (1) Original seed preparation

[0058] a. Preparation of stock matrix

[0059] Choose corn kernels without mildew and rot, add water to boil until the endosperm has a white heart, filter, add 1% gypsum and 0.15% MgSO according to the mass percentage of corn kernels 4 , 0.3% KH 2 PO 4 , Stir and mix well, put it into a 650mL original seed bottle, sterilize at 121°C, 103-115kPa for 1.5h, filter, and cool to room temperature.

[0060] b. Vaccination

[0061] Inoculation needs to be performed on a clean bench. Before inoculation, the inoculation room should be sterilized by ultraviolet light. First, use 75% alcohol to sterilize the hands and inoculation needles. The inoculation needles, test tube ports and tampons must be sterilized with the flame of an alcohol lamp. Select strains that grow vigorously, have a relatively short age, and have no pigment secretion in the mycelium layer for inoculation, and use an inoculation needle to pick 1 cm from the mother seed test t...

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Abstract

The present invention discloses an artificial cultivation method for stone-like omphalia. The artificial cultivation method specifically comprises: breeder seed preparation, cultivated seed preparation and indoor or outdoor soil covered cultivation. The method has the advantages that: corn is used as a main raw material for preparing breeder seeds of stone-like omphalia, and the problem that hyphae grow slowly in the breeder seed cultivation process is solved; Oak sawdust is used as a main material, soybean meal, wheat bran and corn flour are used as auxiliary materials, and KH2PO4, gypsum and the like are used as mineral elements in the the cultivation, so that the growth speed of stone-like omphalia hyphae is accelerated, and a production cycle is greatly shortened; corn straws and bamao straws are used in indoor and outdoor cultivation, and are cheap and easy to obtained, so that the cost is low, wooden material needs are reduced, and protection of the ecological environment is provided; and stone-like omphalia sclerotium is obtained by a soil covered cultivation technology, the operation is simple and easy, the yield is high, the labor cost is reduced, and the economic benefits of the stone-like omphalia production are increased.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an artificial cultivation method of thunder pills. Background technique [0002] Lei Wan is the dried sclerotia of Omphaliarapidescens Schroet., a fungus belonging to the family Omphaliapidescens Schroet. Leiwan is also known as Zhuling, Leishi, Leishi, Zhulinzi, Zhu Lingzhi, Mulianzi, etc. It can be harvested in spring, autumn, and winter, but it is mostly in autumn. Select the diseased bamboo with withered branches and leaves, and dig out the sclerotia of the roots . The life-span of its fruiting bodies is very short, and the common one is sclerotium. Lei Wan has always been wild, and systematic artificial cultivation research is rarely reported, and based on the following reasons, the existing technology of artificial cultivation of Lei Wan is not enough to guide production. , and it is not easy to observe mycelial growth and pollution; ②The ratio of bass...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
CPCC05B7/00C05D3/02C05D5/00C05F5/002C05F11/00
Inventor 李娜赵银瓶肖波韦中强秦静韩量韩如刚杨毅张万超
Owner 重庆市药物种植研究所
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