Fast check reagent of Salmonella causing neutralization food source type food poisoning

A Salmonella and food-borne technology, applied in the field of rapid diagnostic reagents for patients, can solve the problems that affect the identification of pathogenic bacteria, difficulty, and long cycle, and achieve satisfactory clinical application effect, less pollution, and short time

Inactive Publication Date: 2009-10-28
于维森
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It also seriously affects the confirmation of pathogenic bacteria. It takes experience to select suspicious colonies on the medium for selective isolation and culture, and there is a possibility of missed detection. However, the enterotoxin of Salmonella is very stable, although processed food sometimes cannot be detected. , but there may still be enterotoxin, and the conventional detection method of enterotoxin needs to do animal experiments, which is difficult and takes a long time

Method used

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Embodiment Construction

[0011] Below in conjunction with embodiment the present invention will be described in further detail

[0012] Configure according to the data listed in the following table (grams per liter of distilled water) and the steps described:

[0013] Recipe one:

[0014] Peptone 5.0-10.0; lactose 3.5-4.5; disodium hydrogen phosphate 4.0-5.0; sodium dihydrogen phosphate 5.0-6.0; compound growth factor 10.0-20.0; Sodium sulfate 20.0-30.0; lecithin 0.5-1.0; sterile Salmonella polyvalent and monovalent serum 10-20ml; distilled water was added to 1000mL, and the pH was adjusted to 7.1.

[0015]

[0016] Recipe 2:

[0017] Peptone 6.0-8.0; lactose 3.8-4.2; disodium hydrogen phosphate 4.2-4.7; sodium dihydrogen phosphate 5.2-5.6; compound growth factor 12.0-18.0; Sodium sulfate 24.0-28.0; lecithin 0.6-0.8; sterile Salmonella polyvalent and monovalent serum 12-18ml; distilled water was added to 1000mL, and the pH was ...

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Abstract

The invention relates to a fast check reagent of Salmonella causing neutralization food source type food poisoning, in particular to a fast diagnosis reagent for a patient with most common Salmonella causing food source type food poisoning after a plurality of antibiotics are clinically used and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum and a neutralizing agent are added in enriched liquid, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the neutralizing agent can neutralize a plurality of residual antibiotics or disinfectors contained in a specimen and generate a neutralized product having no influence on the growth of Salmonella. The identification accuracy is larger than 99 percent, the fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria; and the check diagnosis process is shortened to 2-6 hours.

Description

technical field [0001] The present invention relates to a rapid detection reagent for neutralizing food-borne food poisoning Salmonella, especially the rapid diagnostic reagent for patients with food-borne food poisoning caused by the most common Salmonella after a large amount of clinical use of antibiotics. The culture test of Salmonella is Important method for diagnosing foodborne food poisoning. Especially for the elderly, children and infirm with acute gastroenteritis, if the first aid treatment is not carried out in time, it can also lead to death. The correct diagnosis can only be made after the specimen is cultured; the cured patients need to be cultured to confirm whether they are cured. The rapid test reagent can detect the positive results of Salmonella after a large number of clinical antibiotics are used, has the function of guiding clinical diagnosis and treatment, and is also of great significance in preventing the abuse of antibiotics. The traditional routine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10
CPCY02A50/30
Inventor 于维森
Owner 于维森
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