Method for studying striga asiatica lactone in regulating rape root system growth

A technology for strigolactone and root growth, which is applied in the directions of seed and rhizome treatment, botanical equipment and methods, soilless cultivation, etc., and can solve the problem of lack of technical data for strigolactone growth of rapeseed, difficulties in mutants, There are no problems such as the technology or literature for creating mutants, which can reduce the experimental error and workload, promote the growth of rapeseed, and increase the number of root tips.

Inactive Publication Date: 2017-09-29
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are two problems in the previous research: first, compared with model plants such as rice and Arabidopsis, rape is allotetraploid, and it is difficult to obtain muta

Method used

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  • Method for studying striga asiatica lactone in regulating rape root system growth
  • Method for studying striga asiatica lactone in regulating rape root system growth
  • Method for studying striga asiatica lactone in regulating rape root system growth

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Example Embodiment

[0036] Example 1:

[0037] A method for studying strigolactone regulating rape root growth, the steps are:

[0038] (1) Optimization of hydroponic conditions and strigolactone concentration:

[0039] A: Select Zhongshuang 11 and Zhongyouza 12 rape seeds, soak them in 70% (volume ratio) ethanol for 10 minutes, and rinse them with distilled water for 6 times;

[0040] B: The seeds are evenly placed on a petri dish covered with 3 layers of wet filter paper, and germinated in a constant temperature growth box at 22°C for 2 or 3 days, with 16 hours of light and 8 hours of darkness to keep the filter paper moist;

[0041] C: Take several plastic cups with a diameter of about 10 cm and fill them with distilled water, cover the mouth of the cup, fix the gauze and soak it in water, place the germinated rapeseed evenly on the gauze, and after 3 days, when the hypocotyl is about 2 cm long , Take the neatly grown vegetable seedlings and fix them on the foam board, 24 seedlings per board...

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Abstract

The invention discloses a method for studying striga asiatica lactone in regulating rape root system growth. The method comprises the steps that 1, the rape seeds are soaked into ethyl alcohol and rinsed with distilled water; 2, the seeds are evenly put on a culture dish of wet filter paper for germination accelerating under illumination in a constant-temperature growth box; 3, a plastic cup of which the diameter is filled with the distilled water is taken, a cup opening is covered with gauze, the gauze is soaked into water, rapeseeds after germination accelerating are put on the gauze, and rape seedlings with neat growth are fixed to a cystosepiment, and are cultured in a 1/2 Hoagland's nutrient solution; 4, the rapes are moved to striga asiatica lactone solutions with different concentrations for growth, and are sampled every day using a root system scanner to be scanned; 5, rape groups are treated, and materials which are extremely sensitive and insensitive to striga asiatica lactone are screened out. The method for studying striga asiatica lactone in regulating the rape root system growth is feasible and convenient to operate, better facilitates research of shapes of plants, is harmless to root systems, screens the materials of which the root system growth has different sensitivity to striga asiatica lactone, and lays a foundation for later physiology and biochemistry, molecular biology and thremmatology research.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and more specifically relates to a method for studying the growth of rapeseed roots regulated by strigolactone, optimizing the germination conditions of rapeseed seeds, and screening out The most suitable treatment concentration was used to treat natural population seeds from different sources, and to screen mutants whose root growth was sensitive and insensitive to strigolactone. This invention is the first to study the effect of strigolactone on the growth of rapeseed, which provides a technical and method reference for the screening of plant hormone-sensitive differential materials. Background technique [0002] Crop yield physiology is formed through two physiological processes, namely absorption and photosynthesis (Liu Xiaobing et al., 2010). The effective branches and leaves of the aboveground part of the crop form a canopy, which affects the absorption of light energy and the dis...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00
CPCA01C1/00A01G31/00
Inventor 马霓张丰琪张春雷万林
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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