A kind of method that promotes the rapid propagation of succulents in vitro

An in vitro technology of succulent lotus, applied in the field of plant tissue culture, can solve the problems of severe pest infestation, root and stem decline, and yield reduction, and achieve the effects of short cultivation period, vigorous plant growth, and strong division ability

Inactive Publication Date: 2019-02-22
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The subterranean stems are mostly used for reproduction in production, but after 2 to 3 years of continuous propagation, the rhizomes gradually decline, the yield decreases, and the infestation of diseases and insect pests is serious, so it needs to be renewed and propagated.

Method used

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  • A kind of method that promotes the rapid propagation of succulents in vitro
  • A kind of method that promotes the rapid propagation of succulents in vitro
  • A kind of method that promotes the rapid propagation of succulents in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Effects of BA, TDZ and NAA Contents in the Proliferation Medium on the Proliferation of Radix Sinensis

[0067] The test was set up in test groups 1-9. In test groups 1-9, the induction medium was: MS medium was used as the basal medium, 4.0mg / L of BA, 0.1-0.2mg / L of TDZ, 0.9- A medium made of 1.0mg / L NAA, 25-35g / L sucrose and 6.5-7.5g / L agar; the matrix is ​​a mixture of yellow mud, peat and sand with a weight ratio of 3:2:1 In the value-proliferating medium, the content of sucrose is 25-35 g / L, and the content of agar is 6.5-7.5 g / L.

[0068] In the medium kits used in test groups 1-9, the induction medium and matrix were the same, and the contents of sucrose and agar in the value-proliferation medium were also the same. The only difference in the medium kits used in test groups 1 to 9 is the content of BA, TDZ and NAA in the value-added medium, which are as follows:

[0069] group

BA content (mg / L)

TDZ content (mg / L)

NAA content (mg / L)

T...

Embodiment 2

[0083] Effect of TDZ Content in Proliferation Medium on the Proliferation of Radish

[0084] Test set-up: Test groups 1-2. In test groups 1-2, the induction medium is: MS medium as the basal medium, adding 4.0mg / L of BA, 0.1-0.2mg / L of TDZ, 0.9- A medium made of 1.0mg / L NAA, 25-35g / L sucrose and 6.5-7.5g / L agar; the matrix is ​​a mixture of yellow mud, peat and sand with a weight ratio of 3:2:1 In the value-proliferating medium, the content of sucrose is 25-35 g / L, and the content of agar is 6.5-7.5 g / L.

[0085] In the medium kits used in test groups 1-2, the induction medium and matrix were the same, and the contents of BA, NAA, sucrose and agar in the proliferation medium were also the same. The only difference between the medium kits used in test groups 1-9 is the content of TDZ in the value-proliferating medium, which are as follows:

[0086] group

BA content (mg / L)

TDZ content (mg / L)

NAA content (mg / L)

Test group 1

2.0

0.01

0.05

...

Embodiment 3

[0096] A kind of embodiment of the culture medium box that promotes the in vitro rapid propagation of Radix Miscellanea of ​​the present invention, the culture medium box of described embodiment comprises induction medium, value-proliferating medium, matrix;

[0097] The induction medium: based on MS medium, added BA of 4.0mg / L, TDZ of 0.1mg / L, NAA of 0.9mg / L, 25g / L sucrose and 6.5g / L agar Culture medium;

[0098] The value-proliferating medium: based on MS medium, added 2.0mg / L of BA, 0.01mg / L of TDZ, 0.04mg / L of NAA, 35g / L of sucrose and 7.5g / L of agar Culture medium;

[0099] The substrate: a substrate obtained by mixing yellow mud, peat and sand with a weight ratio of 3:2:1.

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Abstract

The invention discloses a culture medium box set for promoting in-vitro rapid propagation of zingiber mioga. The culture medium box set is characterized in that an MS culture medium is used as a basic culture medium, and is added with 1.0 to 3.0mg / L of BA (butyl acrylate), 0.01 to 0.02mg / L of TDZ (thidiazuron), 0.04 to 0.06mg / L of NAA (naphthalene acetic acid), 25 to 35g / L of sugarcane and 6.5 to 7.5g / L of agar, so as to obtain the propagation culture medium. The invention also discloses a method using the culture medium box set to promote the in-vitro rapid propagation of the zingiber mioga. The method has the advantages that under the optimizing conditions, after the zingiber mioga is cultured by the method,; 10 to 15 propagation times can be reached; after 30 days, the rooting rate is 100%; the transplanting survival rate is 100%.

Description

technical field [0001] The invention relates to a method for culturing plant tissue, in particular to a method for promoting rapid propagation of succulents in vitro. Background technique [0002] Zingiber mioga (Thunberg) Roscoe is a perennial herb belonging to Zingiberaceae Zingiberaceae, also known as Zingiber mioga (Thunberg) Roscoe, and has two types of purple flowers and white flowers. The lotus has high edible value and medicinal value. It mainly eats its tender inflorescences, buds, flower rachis and underground stems, among which, every 100g of tender stems and flower rachis contains about 12.4g of protein, 2.2g of fat, and 28.1g of cellulose. g. Vitamin C and vitamin A are about 95.85mg in total, and it also contains iron, zinc, selenium and other trace elements; the rhizome of the lotus root is warm in nature, pungent in taste, warms the middle and regulates qi, dispels wind and relieves pain, can reduce swelling, promote blood circulation, and disperse stasis ; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡秀
Owner ZHONGKAI UNIV OF AGRI & ENG
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