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A kind of tissue culture method of Yulong fern

A technique of tissue culture and Yulong fern, which is applied in the field of plant tissue culture, can solve the problems of difficult reproduction and no report on the reproduction of Yulong fern, and achieve the effects of reducing the process flow, shortening the seedling cultivation time, and rapidly multiplying

Active Publication Date: 2022-07-01
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to better protect Yulong fern and its subsequent development and utilization, the first key task is to solve the problem of its difficult reproduction. There is no report on the reproduction of Yulong fern.

Method used

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  • A kind of tissue culture method of Yulong fern
  • A kind of tissue culture method of Yulong fern
  • A kind of tissue culture method of Yulong fern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for tissue culture of Yulong fern, comprising the following steps:

[0038] (1) Germination of the spores of fern japonica; sterilize the mature spores of fern japonica with a 0.1% mercuric solution for 5 minutes, rinse with sterile water for 3 to 5 times, and inoculate in 1 / 8MS medium for culture Germination was performed to obtain clump-like gametophytes; the temperature for germination was 20°C, the light intensity for germination was 1500-2000 Lx, and the light time for germination was 12 hours / day.

[0039] (2) Induce seedlings; divide the gametophytes (cultivated for 90 days) of the Yulong fern obtained in step (1) into a size of 0.5-1.0 cm in length and width, inoculate them in 1 / 2MS medium for culture, and culture for about 66 days to obtain sporophytes , its induction rate is above 95%.

[0040] (3) Proliferation: Cut the sporozoite clumps that have been cultured for about 45 days into a size of 0.5-1.0 cm in length and width, inoculated in 1 / 2 MS med...

Embodiment 2

[0044] A method for tissue culture of Yulong fern, comprising the following steps:

[0045] (1) Germination of the spores of the fern japonica; sterilize the mature spores of the fern japonica with a mass concentration of 0.1% mercuric solution for 6 minutes, rinse with sterile water for 3 to 5 times, and inoculate in 1 / 8MS medium for cultivation Germination to obtain clump-like gametophytes; the temperature for germination is 15°C, the light intensity for germination is 1500-2000 Lx, and the light time for germination is 12 hours / day.

[0046] (2) Induce seedlings; divide the gametophytes (cultivated for 90 days) of the Yulong fern obtained in step (1) into a size of 0.5-1.0 cm in length and width, inoculate them in 1 / 2MS medium for culture, and culture for about 66 days to obtain sporophytes , its induction rate is above 95%.

[0047](3) Proliferation; the sporozoite clumps cultured for about 45 days are cut into a size of 0.5-1.0 cm in length and width, and inoculated into...

Embodiment 3

[0051] A method for tissue culture of Yulong fern, comprising the following steps:

[0052] (1) Germination of the spores of fern japonica; sterilize the mature spores of fern japonica with a 0.1% mercuric solution for 5 minutes, rinse with sterile water for 3 to 5 times, and inoculate in 1 / 8MS medium for culture Germination was carried out to obtain clump-like gametophytes; the temperature for germination was 18°C, the light intensity for germination was 1500-2000 Lx, and the light time for germination was 12 hours / day.

[0053] (2) Induce seedlings; divide the gametophytes (cultivated for 90 days) of the Yulong fern obtained in step (1) into a size of 0.5-1.0 cm in length and width, inoculate them in 1 / 2MS medium for culture, and culture for about 66 days to obtain sporophytes , its induction rate is above 95%.

[0054] (3) Proliferation; the sporozoite clumps cultured for about 45 days are cut into a size of 0.5-1.0 cm in length and width, and inoculated into 1 / 2 MS medium...

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Abstract

The invention discloses a method for tissue culture of Pteridophyta, comprising the following steps: (1) germination of spores of Pteridium japonica; inoculating the spores of Pteridophyta japonica in a germination medium for culturing and germination to obtain clump-shaped gametophytes; (2) inducing into seedling; inoculate the gametophyte in an induction medium and cultivate to obtain a sporophyte; (3) multiply; inoculate the sporophyte in a subculture medium and cultivate to obtain a clump seedling; (4) strong seedling; The clump seedlings are inoculated in 1 / 2 MS medium for cultivation to obtain rootless seedlings; (5) transplanting and refining the seedlings; The advantages of the present invention are that: the efficient propagation system of the seedlings of the jade dragon fern is successfully constructed, the artificial rapid propagation of the jade dragon fern is realized, and the technical support is provided for its protection, development and utilization.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method of Yulong fern. Background technique [0002] Jade dragon fern (Polystichum glaciale Christ) is a small perennial herbaceous fern of the genus Auricularia genus of the family Pteridaceae, which is a unique alpine species in my country. The number of Yulong fern is rare and on the verge of extinction. It is listed as a national level I key protected plant, a plant with a very small population, and is listed as a near-threatened (NT) plant in the "World Conservation Union Red List of Threatened Species" (IUCN). It is only distributed in Yunnan, Sichuan, Tibet and other places, and is born in glacial caves, cliffs and rock crevices at an altitude of 3200-4700 meters. The scales of the jade dragon fern are reddish-brown at first, and turn pale white in old age. Yulong fern has a beautiful plant shape and can be developed as a small b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/28
CPCA01H4/008A01G24/28
Inventor 向建英和秋兰孙大宽段杰秋唐军荣刘琳
Owner SOUTHWEST FORESTRY UNIVERSITY
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