The invention discloses a method for comprehensively culturing cistoclemmys flavomarginata by incubating from breeder to adult. The method comprises the following steps: 1) breeder, namely a, breeder identification; b, breeder raising; c, breeder mating; and d, spawning and inoculation; 2) infants, namely a, infant early raising; b, infant later raising; and c, infant overwintering; 3) young turtles, namely a, stocking; b, diet feeding; c, daily management; and d, young turtle overwintering; and 4) adults, namely a, stocking; b, diet feeding; c, daily management; and d, adult overwintering. The culturing method can be used for increasing the survival rate of cistoclemmys flavomarginata to 90 percent so as to greatly reduce the death rate in infant period and young turtle period, and carrying out comprehensive culturing from the source, and has the advantages of high breeder mating efficiency, high fertilized ovum quality, scientific management, high adult quality, high ornamental value and high medicinal value.