19results about How to "Achieve activity" patented technology

The application discloses a method for preparing cell-derived exosomes and products and applications thereof, and the method comprises the following steps: co-culturing immune-activated programmable cells with a stimulating agent with immune-activation effect, and collecting the cell-derived exosomes with immune activity by a natural secretion collection method or an artificial extrusion collection method. The application has safe and controllable local immune activation, and the immune activation strategy based on the exosomes fully overcomes the safety bottleneck of traditional live bacterial preparations in tumor treatment. By replacing live bacteria with engineered exosomes, not only potential risks such as systemic inflammatory response and infection are effectively avoided, but also the local controllability of immune stimulation signals is ensured, the precise activation and directional regulation of tumor region immune cells are realized, the tumor microenvironment is reshaped, the anti-tumor effect is strengthened, and the broad-spectrum cross-tumor potential is verified across models.

The invention relates to the technical field of bacterial cellulose preparation, in particular to a composite flora for producing bacterial cellulose and a preparation method thereof, and the preparation method comprises the following steps: screening gluconobacter oxydans, obtaining culture medium preparation materials and preparation tools, preparing a slant culture medium set, preparing an enriched liquid culture medium set, and preparing composite flora mixed inoculation liquid. The method comprises the following steps: preparing a waste tobacco leaf leaching solution, extracting a proportional leaching solution from the waste tobacco leaf leaching solution according to a preset proportion, obtaining a mixed fermentation culture medium based on a preparation tool and the proportional leaching solution, and carrying out inoculation operation on a composite flora mixed inoculation solution by using the mixed fermentation culture medium to obtain a dry cellulose membrane; calculating the yield of the bacterial cellulose based on the dry cellulose membrane, and completing the preparation of the composite flora for producing the bacterial cellulose based on the yield of the bacterial cellulose. The method can be used for scientifically, efficiently and stably preparing the composite flora.

The application belongs to the technical field of biological medicine, and discloses an in-situ vaccine type mRNA-tLNP preparation for solid tumor treatment and preparation and application thereof. The preparation is designed by double targets, so that CAR-T kills more tumor cells to overcome tumor heterogeneity, changes the tumor immune microenvironment through autocrine fusion proteins (anti-PD-1 scFv and TGFbetaRII, IL-15 and Flt3L, CD40L), promotes T cell proliferation, and recruits and activates dendritic cells, cross-presents new antigens in the tumor local part, and produces in-situ vaccination effects; circular RNA encoding the above proteins is prepared, and the circular RNA is wrapped by lipid nanoparticles modified by CD3 antibodies, so as to be directly delivered to the body, and a significant solid tumor treatment effect is produced.

This application provides a method for regenerating ternary cathode materials based on acid etching and molten salt lithium replenishment, as well as the regenerated electrode material and battery. The method includes the following steps: pretreating the failed ternary battery cathode material with an organic acid solution; mixing the pretreated failed ternary battery cathode material, dopant, and lithium-containing molten salt, and performing a first-stage low-temperature molten salt heat treatment at a first specified temperature; further subjecting the material after the first-stage low-temperature molten salt heat treatment to a second-stage high-temperature heat treatment at a second specified temperature for 2-4 hours; and washing and drying the product after the second-stage high-temperature heat treatment to obtain the regenerated ternary cathode material. The wet acid etching process selectively removes rock salt phase impurities and contaminants from the electrode material surface, achieving interface cleaning and activation; the molten salt method for lithium replenishment using multi-element synergistic doping introduces multiple beneficial elements for bulk phase doping while replenishing lithium, thereby simultaneously strengthening the material's crystal structure and interface stability.

The invention relates to the technical field of preparation of concrete, in particular to LC3-basalt fiber regenerated block concrete and a preparation method thereof.The preparation method comprises the following preparation steps that S1, LC3 cement, regenerated blocks, natural sand and gravel are poured into a stirrer to be subjected to uniform dry mixing, and a dry mixture is obtained; s2, adding water, a modified water reducer and a performance modifier into the dry mixture, performing wet mixing, uniformly scattering modified basalt fibers during wet mixing, and performing uniform stirring to obtain a wet mixture; and S3, pouring the wet mixture into a test mold, standing at room temperature, demolding, and curing for 26-28 days to obtain the LC3-basalt fiber regenerated block concrete. Through the synergistic effect of the modified basalt fiber, the modified water reducer and the performance modifier, the compressive strength, the strength retention rate after high temperature and the tensile strength of the concrete are remarkably improved, and chemical activation and densification after the regenerated block is added are achieved.

The invention belongs to the technical field of semiconductor manufacturing, micro electro mechanical systems and precise optical coating, and particularly discloses a method for activating the surface of a substrate in situ in an atomic layer deposition chamber, which comprises the following steps: selecting a donor substrate and a target substrate to be activated; etching the donor substrate to obtain a donor substrate with high-density hydroxyl groups; placing the donor substrate with the high-density hydroxyl groups and a target substrate to be activated in the same atomic layer deposition process chamber; and inert carrier gas is introduced into the atomic layer deposition process chamber, purging process parameters are controlled, and in-situ activation of the target substrate is completed. According to the method for activating the surface of the substrate in situ in the atomic layer deposition chamber, after the surface of the substrate is activated by the method, protective films such as aluminum oxide and the like are subjected to atomic layer deposition, so that the adhesive force and long-term reliability of the protective films on a whole device can be ensured; and interface engineering technical support is provided for manufacturing of high-performance and high-reliability three-dimensional heterogeneous integrated devices and micro-nano sensors.

This invention focuses on the application of a PtZn / Al2O3 bimetallic catalyst in the propane dehydrogenation reaction. A uniquely structured mixed oxide support was synthesized through a co-precipitation strategy incorporating an appropriate amount of silicon (Si), and metallic Pt was loaded using an equal-volume impregnation method. Benefiting from the uniform dispersion of Zn, Pt is atomically dispersed on the surface of Zn nanoclusters, improving metal utilization and catalytic efficiency. The introduction of Si significantly optimizes the metal-support interaction, greatly enhancing the catalyst's activity and stability. With catalyst particles of 20-40 mesh, it exhibits excellent catalytic performance, along with good high-temperature stability and resistance to carbon deposition. This preparation process is simple, reproducible, and achieves efficient and highly selective propylene production with low amounts of precious metals, demonstrating promising industrial application prospects.

The utility model discloses a column-free magnetic cell sorting device which comprises a magnetic sorting mechanism, bases and moving mechanisms, the bases and the moving mechanisms are positioned on two sides of the magnetic sorting mechanism, each base is provided with one moving mechanism, the magnetic sorting mechanism comprises a supporting component, a magnetic component and a metal sleeve, and the magnetic component is fixedly arranged in the metal sleeve; a magnet structure of the magnetic assembly is a Halbach array, the top surface of the supporting assembly is used for placing a storage container in which a sample solution is stored, the supporting assembly comprises a base plate and a metal plate, a through hole penetrating through the length direction of the base plate is formed in the base plate, and the metal plate is accommodated in the through hole; the moving mechanism is used for pushing the metal plate to move in the length direction of the through hole. The column-free magnetic cell sorting device disclosed by the utility model can be used for effectively and controllably activating and shielding a magnetic field, and ensuring that an invalid magnetic field is weakened and the magnetic field intensity of a sorting area is enhanced in a working state, so that the magnetic cell sorting effect is improved.

This invention discloses a five-layer electrodeposited high-sealing corrugated pipe based on ultrasound assistance and its preparation method, belonging to the technical field of metal corrugated pipe manufacturing. It aims to solve the problem that in multi-layer electrodeposited corrugated pipes, it is difficult to effectively coordinate current density, surface quality, and interfacial bonding strength, leading to insufficient densification of the deposited layer, unstable interlayer bonding, poor wall thickness uniformity, and failure to meet airtightness standards, ultimately resulting in poor overall performance stability. This invention includes a five-layer composite structure in which the pipe wall is composed of alternating nickel-cobalt alloy and copper layers. Each layer of the five-layer composite structure undergoes ultrasonic vibration treatment during deposition. The corrugated pipe prepared by this invention has a uniform and dense deposited layer with good interlayer bonding. The five-layer alternating deposition layer composite structure not only achieves uniform stress transmission and dispersion, effectively delaying crack propagation and interfacial penetration, but also improves the uniformity of wall thickness distribution, enabling the corrugated pipe to exhibit higher structural stability and long-term reliability in a sealed environment.

This invention provides a collaborative control method based on consciousness impairment assessment and monitoring and synchronous neural stimulation. The method includes: an eye-tracking monitoring and analysis system extracting eye-tracking feature data from eye-tracking signals using image preprocessing and eye-tracking feature extraction algorithms; a server-side management system combining scale assessments and eye-tracking feature data, utilizing a multi-source data fusion assessment algorithm to output the patient's consciousness state assessment results and stimulation parameter information; a neural correlation electrical stimulator receiving the stimulation parameter information and using a timing synchronization control algorithm to synchronously initiate neural electrical stimulation with the eye-tracking monitoring and analysis system; and the server-side management system dynamically adjusting the consciousness state assessment results and stimulation parameter information based on post-stimulation eye-tracking response characteristics and stimulation parameter feedback, reusing the multi-source data fusion assessment algorithm to form a closed-loop control. This invention achieves timing synchronization and command closed-loop through a collaborative linkage algorithm, ensuring efficient collaborative operation of multiple modules and adapting to complex clinical scenarios such as ICUs and rehabilitation departments.

This invention provides a reciprocating piston compressor and a refrigeration device. The reciprocating piston compressor includes: a cylinder seat with a cylinder bore; a piston located within the cylinder bore and sliding reciprocally within the cylinder bore under the drive of a crankshaft; an annular groove is provided around the outer circumference of the cylinder bore within the cylinder seat; and an oil-pressing structure is constructed on the region of the cylinder seat adjacent to the cylinder bore. The oil-pressing structure can deliver a portion of the lubricating oil splashed during crankshaft rotation into the annular groove. This invention, through the oil-pressing structure, delivers a portion of the lubricating oil splashed during crankshaft rotation into the annular groove surrounding the outer circumferential wall of the cylinder bore. The lubricating oil entering the annular groove can reliably dissipate heat and cool the bore wall, thereby effectively reducing the temperature of the piston's outer wall.

This invention discloses a cleaning device for tile bonding surfaces, relating to the field of tile cleaning technology. This invention addresses the shortcomings of existing technologies, which cannot comprehensively clean the gaps and sidewalls of tile bonding surfaces on walls, and suffer from problems such as high probability of glaze damage and incomplete cleaning. On one hand, through the coordinated operation of the walking positioning module and the grout cleaning execution component, it achieves refined cleaning and interface activation of the entire depth and surface of the tile bonding surface, thoroughly removing various impurities that affect the adhesion performance of the grout. On the other hand, through a directional closed dust suction module, it achieves source control and real-time collection of grout cleaning dust, preventing the spread of dust during operation and optimizing the construction environment. An angle-adjustable adsorption and fixing module enables stable self-fixation of the device in various scenarios such as walls and corners, eliminating the need for continuous manual support, significantly reducing work intensity, and broadening the applicable scenarios of the device.

This invention provides a soil conditioner for high geological background and its preparation method, comprising the following steps: (1) mixing and ball milling biochar and calcium oxide to obtain mixed powder one; (2) soaking attapulgite or reduced iron powder in plant extract, adding citric acid to control the pH of the reaction system between 2 and 4 and maintaining it, rinsing with a large amount of water until the pH of the rinsing solution is constant and drying to obtain mixed powder two; (3) mixing mixed powder one and mixed powder two evenly to obtain the soil conditioner, with a mixing time of less than 5 min. The high geological background soil conditioner prepared by this invention is applied to soil remediation. Without adding exogenous Se agents, it achieves the synergistic effect of stabilizing endogenous Cd and activating endogenous Se in the soil by using a low-cost, Se-free green conditioner.

The invention provides an electronic-grade vitamin C trace iron ion directional capture and multistage membrane composite purification system and method, and relates to the technical field of semiconductor high-purity reagent preparation.The method comprises the steps that firstly, a heterogeneous composite membrane system based on a conductive polymer is constructed, and a positive polarization gradient is formed on a membrane interface through a potential trap model; the reduction effect of vitamin C on iron ions is inhibited by utilizing an electronic shielding effect, and the problem of capture failure caused by unstable valence state of impurities under a strong reduction matrix is solved; then, an acoustic flow field enhanced mass transfer mechanism is introduced, mass transfer boundary layer resistance under ppb-level concentration is broken through micro vortexes generated by high-frequency acoustic microflow, and active directional impacting and capturing of trace impurities are achieved; and finally, irreversible locking is carried out based on orbital hybridization in a nanometer confinement range, system self-adaptive adjustment is achieved in cooperation with a PID acoustoelectric coupling compensation algorithm, the limitation of traditional adsorption equilibrium is effectively broken through, and deep removal and semiconductor-grade purification of trace iron ions are achieved.

This invention belongs to the field of physical therapy technology for Parkinson's disease, and provides a non-invasive closed-loop feedback vagus nerve electrical stimulation system for the prevention and treatment of Parkinson's disease. The system includes: a high-frequency electrical stimulator hardware subsystem, transcutaneous nerve stimulation electrodes, a respiratory and heart rate signal acquisition and closed-loop feedback subsystem, and a high-frequency electrical stimulator software subsystem. This invention uses kilohertz stimulation pulses to bidirectionally modulate the vagus nerve, activating afferent C fibers and initiating the anti-inflammatory neural pathway of locus coeruleus noradrenergic neurons, thus alleviating central inflammation. Simultaneously, it blocks A and B efferent fibers, inhibiting mediated α-synuclein retrograde axonal transport. Through multimodal physiological signal monitoring and embedded intelligent algorithms, it achieves real-time, closed-loop, and personalized control of stimulation parameters and automatic switching of stimulation modes, optimizing efficacy and improving safety. Through adhesive electrodes and acquisition devices, it achieves completely non-invasive, wearable, and home-based treatment.

The application discloses a kind of drug composition for pancreatic cancer liver metastasis under the background of metabolism-related fatty liver disease and preparation method and application thereof, the drug composition is with double-layer fat molecule spherical liposome as carrier, photosensitizer is loaded in the bilayer of liposome, chemotherapeutic drug gemcitabine is loaded in the cavity of liposome, gene therapy drug SiRNA CD44 is adsorbed on the surface of liposome.The GIR-Lip of the application can realize controllable killing and immune activation of tumor under laser irradiation, efficiently silence the high invasion and migration ability of CD44 tumor cells, combined with the first-line chemotherapeutic drug gemcitabine for pancreatic cancer, can ensure the combined effect, make up the drug resistance and curative effect loss caused by long-term use of gemcitabine.

The present application belongs to the field of mineral resource processing, and particularly relates to a pretreatment and valuable element extraction method for seafloor polymetallic nodules, which comprises the following steps: rapidly heating and heat-insulating pretreatment of a mixture containing seafloor polymetallic nodules, carbon material and additives by means of electric resistance heating, to obtain pretreated material; wherein the heating rate of rapid heating is 100-500 DEG C / s; the pretreatment temperature is 800-1000 DEG C; and the additives contain at least one of alkali metal salt, alkaline earth metal salt or ammonium salt. The seafloor polymetallic nodules, carbon material and additives are innovatively instantaneously heated and pretreated, which can optimize the physicochemical structure of seafloor polymetallic nodules, and further optimize the subsequent leaching behavior, and improve the extraction rate of valuable elements.

The application discloses a traffic emergency command retrieval enhancement generation method based on static and dynamic double-layer knowledge base enhancement. In view of the problems of existing RAG technology, such as static and dynamic knowledge splitting, lack of regulation constraints and strategy verification, a static knowledge base including a basic regulation sub-library and a historical case sub-library, and a dynamic knowledge base including a real-time situation sub-library and a prediction and deduction sub-library are constructed; a four-level knowledge graph of events, scenes, strategies and effects is established; after receiving an emergency event, multi-dimensional timeliness activation weights including emergency degree, freshness, spatial correlation and historical similarity are calculated to activate dynamic knowledge, and cross-layer attention mechanism is used to associate static knowledge for multi-target fusion sorting; the fused knowledge is input into a large language model, candidate strategies are generated by combining regulation Trie tree constraint decoding; and the optimal strategy is output after sand table deduction and evaluation by a traffic digital twin model, and feedback closed-loop optimization is carried out based on actual disposal effect.