39results about How to "Strong ability to split" patented technology

The invention discloses a splitter for rock boring, which comprises a chassis, a large arm, a small arm and a control system, and is characterized by further comprising a propelling beam, a hydraulic rock drill, a propelling oil cylinder, a splitting machine and a switching mechanism, wherein the propelling beam is connected with one end of the small arm through an angle adjusting device; both the hydraulic rock drill and the propelling oil cylinder are arranged on the propelling beam; the propelling oil cylinder drives the hydraulic rock drill to slide on the propelling beam; the switching mechanism is connected to one side edge of the propelling beam; the splitting machine is arranged on the switching mechanism. The splitter is high in efficiency and high in splitting effect.

The invention discloses an inducing culture method of an Aconitum vilmorinianum Kom. embryoid. The inducing culture method includes the steps that bulbils of Aconitum vilmorinianum Kom. are used as explants, after the bulbils are disinfected, callus is first induced, and embryonic callus with the color of light yellow with green and vigorous division is selected from the callus for inducing culture, and obtain the Aconitum vilmorinianum Kom. embryoid is obtained. A new way is provided for the mass cultivation and rapid propagation of high-quality seedlings of the Aconitum vilmorinianum Kom., and the problems of the shortage of plant resources and seedling resources of the Aconitum vilmorinianum Kom. as well as serious diseases and the like can be effectively solved.

The invention provides a human alveolar epithelial cell separation and culture method. Collected tissue is surrounded by an activated substrate to effectively grow adhering to the wall, serum ingredients can inhibit division and differentiation of alveolar epithelial cells, and therefore, the cell viability is greatly improved; tissue fragments are subjected to shake culture in a specific gaseousenvironment, damage, caused by ischemia, of the collected tissue sample can be effectively avoided, and subsequent culture operation is facilitated. Ureidohydantoin, creatine and asiaticoside added into a passage culture medium can effectively improve the cell viability and the division capacity, and therefore, cell growth and proliferation are promoted. 3T3 cells serve as the trophoderm, a good growth substrate can be provided for the primary alveolar epithelial cells, growth and division of the alveolar epithelial cells can be effectively promoted, and therefore, the culture effect is greatly improved. Through the method, the viability and the proliferation speed of the alveolar epithelial cells can be remarkably increased, and therefore, high-quality experimental materials are providedfor related research projects.

The invention provides a preparation method and application method of a naphthidine molecularly-imprinted polymer. The preparation method provided by the invention comprises the following steps: dissolving S-1,1-binaphthyl-2-amine and methacrylic acid into a pore-forming agent, then feeding nitrogen for 5 minutes under an ultrasonic condition, and then carrying out pre-polymerization for 10 hours at the temperature of 18 DEG C and at normal pressure, so that the S-1,1-binaphthyl-2-amines and the methacrylic acid fully act so as to form a compound; adding ethylene glycol dimethacrylate and azo-bis-iso-butyrynitrile, and then feeding nitrogen for 5 minutes under the ultrasonic condition; placing the obtained product in a water bath kettle at the constant temperature of 60 DEG C to polymerize for 12 hours so as to obtain a bulk molecularly-imprinted polymer; crushing and grinding the obtained bulk molecularly-imprinted polymer; sieving with a sieve of 60mu m-20mu m meshes, and then carrying out repeated settlement with acetone to remove ultra-fine particles; and drying so as to obtain the naphthidine molecularly-imprinted polymer. As a HPLC (high performance liquid chromatography) stationary phase, the imprinted polymer can achieve the chiral separation of racemic DABN (1,1-binaphthyl-2-amine), and the separation factor of the imprinted polymer on the racemic DABN can reach 2.4. The preparation method provided by the invention is high in separation performance, low in cost, simple in operation, and good in stability.

The invention discloses a polyelectrolyte grafted polyvinyl alcohol spinning membrane for oil-water emulsion separation and a preparation method and application thereof.The preparation method comprises the steps that a polymer is dissolved in dimethyl sulfoxide, hexamethylene diisocyanate is added, a third solution system is formed, a cross-linked polyvinyl alcohol spinning membrane is immersed in the third solution system, and the polyelectrolyte grafted polyvinyl alcohol spinning membrane is obtained; treating at a first predetermined temperature for a third predetermined time to obtain a grafted polyvinyl alcohol spinning membrane, and extracting, cleaning and drying the grafted polyvinyl alcohol spinning membrane by using hot dimethyl sulfoxide after the treatment is completed to obtain the polyelectrolyte grafted polyvinyl alcohol spinning membrane. According to the method, emulsion demulsification and oil-water separation operation are combined, an oil-water mixture and oil-water emulsion can be efficiently separated at a high speed, pollution is not likely to happen, the continuous operation capacity is achieved, the emulsion does not need to be demulsified in advance, other substances are not introduced in the collaborative demulsification process, and further recycling and application of separated oil and water are facilitated.

The invention discloses an improvement method for sugarcane stem tip culture, and belongs to the technical field of agricultural production. The improvement method comprises the following steps: a stem tip of a sugarcane tail tip is selected as an explant, heat shock treatment is carried out in sterile water at 40-45 DEG C, the explant is inoculated into an improved MS culture medium, and culturing is performed until a new lateral bud grows; wherein in the culture period, cold stimulation is carried out 1-3 times every day from the fifth day of inoculation, and the cold stimulation lasts for 10-18 days; and the improved MS culture medium comprises the following components: MS, 0.05-0.15 mg/L of thidiazuron, 0.1-0.25 mg/L of 6-BA and 20-30 g/L of cane sugar. According to the improvement method for sugarcane stem tip culture, the highest survival rate of the stem tips reaches 86.35%, the culture period is shortened, and the requirement of the market for a large number of sugarcane asexual propagation materials is met.

The invention discloses a sorbus pohuashanensis stem cell capable of increasing anthocyanin content, a culture medium and a culture method, the culture medium can be applied to the culture for synthesizing anthocyanin from the sorbus pohuashanensis stem cell, and the culture method comprises the following specific steps: (1) preparing stem cell tissues; and (2) induction and accumulation of anthocyanin. According to the method, the konjac is used for preparing a natural culture medium, and nutrient substances of the konjac are used for providing macroelements, microelements and a carbon source for stem cells to be cultured; substances contained in the konjak have a promoting effect on stem cell induction, explants expand in 3-5 days, and stem cells are obviously increased in 7-10 days; the konjak culture medium also has the effect of promoting anthocyanin synthesis, and the anthocyanin content is obviously increased; besides, the konjac glucomannan has good coagulability by utilizing the characteristics of the konjac glucomannan, the addition of agar is reduced, the culture cost is saved, and a target product cultured by the natural culture medium is safer and more credible.

The invention belongs to the technical field of flower seed treatment, and particularly discloses a method for improving butterfly orchid seed germination rate and shortening germinating time. The method specifically comprises the following steps: (1) carrying out freezing treatment on butterfly orchid seeds, taking out the treated butterfly orchid seeds, carrying out stir-frying at constant temperature, taking out the butterfly orchid seeds after stir-frying, soaking the butterfly orchid seeds into a ferric nitrate solution, and taking out a filter liquor; (2) carrying out magnetizing treatment, soaking the seeds in seed soaking solution, fishing out the seeds from the filter liquor, and carrying out microwave drying, wherein the seed soaking solution is prepared from the following raw materials: erythritol, polygahatous polysaccharides, lycium barbarum polysaccharide, robiniae, Chinese toon sprouts, purslane, copper chloride and water. Rapid germination of butterfly orchid seeds is effectively promoted, so that the germination rate reaches 88% or more, the butterfly orchid emergence rate and seedling survival rate are effectively improved, the abloom rate of butterfly orchid seedpropagation can also be improved effectively, the flowering period is prolonged, the occurrence of diseases in the growth period of butterfly orchid can be reduced effectively, the cold resistance isstrengthened, vigorous growth at low temperature can also be realized, and the method is easy to manage.

The invention belongs to a blood-related product, and particularly relates to a human hematopoietic product for oral administration or injection. The main components of the human hematopoiesis comprise essential amino acids, trace elements, vitamins, nutrient substances and growth factors. The preparation method comprises the following steps: synthesizing and sub-packaging in a sterile reaction kettle, diluting with normal saline to form a diluent, mixing with glycerol, lecithin and soybean oil emulsion, inputting into the liquid through mixed gas of medical sterile pure O2 and O3 to enable the oxygen partial pressure to reach 105Kpa or above, filling into an infusion bag at equal pressure to prepare the hematopoietic finished product, and after a pyrogen test is qualified, clinically infusing or orally taking the medicine by a human body. The hematopoietic carbonized fluorine-free compound is prepared from a safe and nontoxic lecithin emulsion; the emulsion which has oxygen carrying capacity, is not limited by blood types, can be stored for a long time, is free of mixing of bacteria or viruses and promotes growth of the hematopoietic stem cells is obtained by diluting a diluent of amino acid, trace elements, vitamins, nutrient substances and growth factors and injecting oxygen at overhigh pressure. The growth factors further promote synthesis of hematopoietic stem cells and division of hemoglobin, the blood recovery capacity is improved through the synergistic effect of multiple raw materials, and the composition is especially suitable for ischemia symptoms such as iron deficiency type anemia.

The invention relates to the technical field of traffic, and discloses an automatic conveying and shearing device for traffic sign manufacturing. The device comprises an operation table, wherein a side plate is arranged on the side face of the operation table, a crankshaft is arranged on the surface of the side plate, a rocker is movably connected to the surface of the crankshaft, a fluted disc is movably connected to the surface of the side plate, a gear is arranged on the lower surface of the fluted disc, a rolling wheel is arranged on the side face of the gear, a ratchet wheel is arranged in the rolling wheel, an auxiliary wheel is arranged in the operation table, and a cam is arranged on the surface of the crankshaft. According to the automatic conveying and shearing device for traffic sign manufacturing, the crankshaft and the rocker are used in a matched mode, the rocker and the fluted disc are used in a matched mode, the gear and the ratchet wheel are used in a matched mode, and the rolling wheel and the auxiliary wheel are used in a matched mode, traffic signs which are not sheared can be automatically and intermittently conveyed at equal intervals, the conveying effect of the traffic signs is good, and the shearing effect of the traffic signs can be improved.

The invention relates to a rapid propagation method for corylus chinensis by inducing clumped buds. According to the technical points, the method is mainly characterized in that seedling stem sections and mature embryos of corylus chinensis serve as explants and are planted in initial media, and after the fixed bud induction rate reaches 96.4% or above, the explants are transplanted into a medium for clumped bud induction, wherein one formula of the initial media comprises MS, 1.0 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, the other formula of the initial media comprises MS, 33.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, and the medium for clumped bud induction is prepared from MS, 1.5 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 0.1 g.L<-1> of tryptone, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC; the germination rate is 92%.

The invention discloses an application of nuclear membrane protein knock-down in stem cell transplantation, which specifically comprises the following steps: 1) designing two pairs of siRNA primers aiming at nuclear membrane protein on line, and constructing a lentiviral vector; (2) intervening proliferation and differentiation of the neural stem cells by in-vitro lentivirus-mediated nuclear membrane protein knock-down; and 3), intervening proliferation and differentiation of the neural stem cells by in-vivo lentivirus-mediated nuclear membrane protein knock-down. According to the invention, LBR (nuclear membrane protein) is used for inhibiting division of stem cells and promoting differentiation of the stem cells; neural stem cells may have strong division ability after transplantation, and have potential carcinogenicity. After the nuclear membrane protein is knocked down, the cell division capability is obviously reduced so that the method can be widely used for solving the carcinogenicity problem caused by stem cell transplantation.

The invention belongs to a blood-related product, and particularly relates to artificial blood for oral administration or injection. The main components of artificial blood comprise essential amino acids, trace elements, vitamins and growth factors. The preparation process comprises the following steps: synthesizing and sub-packaging in a sterile reaction kettle, diluting with normal saline to form a diluent, mixing with soybean oil emulsion, inputting into the liquid through mixed gas of medical sterile pure O2 and O3 to enable the oxygen partial pressure to reach more than 100Kpa, and isobarically filling into an infusion bag to obtain an artificial blood finished product, and after the artificial blood finished product is qualified through pyrogen inspection, the artificial blood can be used for clinical human body infusion or oral administration. The artificial blood is free of carbonated fluorine compounds, through the safe and non-toxic soybean oil emulsion, diluted by the diluent of the amino acids, the trace elements, the vitamins and the growth factors, and injected with oxygen under high pressure, the emulsion which has oxygen carrying capacity, is not limited by blood types, can be stored for a long time, is free of mixing of bacteria or viruses and can promote growth of hematopoietic stem cells is obtained, the growth factors further promotes synthesis of the hematopoietic stem cells and splitting of hemoglobin, the various raw materials have a synergistic effect to improve the blood recovery capability, and the artificial blood is especially suitable for ischemia symptoms such as iron deficiency anemia.