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A tissue culture method for rapid propagation of alum root "Tiramisu"

A tissue culture and medium formula technology, applied in the field of plant tissue culture, can solve the problems of low reproductive efficiency of alum root plants, unfavorable for mass propagation of seedlings, low multiplication rate of adventitious buds, etc., and achieves fast reproduction speed and vigorous metabolic activity. , the effect of good leaf development

Active Publication Date: 2021-05-18
北京花乡花卉科技研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The propagation efficiency of the alum root plants obtained by the conventional alum root propagation method is low and the cycle is long, which is not conducive to mass propagation of seedlings
Propagate plants with budded stems as explants, the contamination rate of explants is high, and continuous taking of budded stems is likely to cause greater damage to the mother plant, and the multiplication rate of adventitious buds obtained by this propagation method is biased. Low
Therefore, there are the problems of slow propagation speed, low multiplication rate and high pollution rate in the propagation mode of above-mentioned alum root

Method used

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  • A tissue culture method for rapid propagation of alum root "Tiramisu"
  • A tissue culture method for rapid propagation of alum root "Tiramisu"
  • A tissue culture method for rapid propagation of alum root "Tiramisu"

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Experimental program
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Effect test

Embodiment 1

[0064] Utilize the cultivation method provided by the invention to carry out rapid propagation to it, and concrete steps are as follows:

[0065] (1) Establishment of the sterile system: Select a mother plant that grows robustly and is free of diseases and insect pests, and place it in the greenhouse for cultivation. Spray 1000 times of carbendazim 7 days before collecting materials; when collecting materials, choose to cut the petioles of new leaves that have grown for 30 days, and wash them with running water Dry for 10 minutes; wipe the petiole with cotton containing 75% alcohol, wipe off the epidermis on the petiole, be careful not to break the petiole when wiping, and wash it twice with sterile water; put the material into 0.1% mercuric chloride solution for disinfection 6 minutes, washed 5 times with sterile water, oscillating for 3 minutes each time, cut the petiole into small sections of 1 cm to obtain sterilized explants.

[0066] (2) Callus induction: inoculate the e...

Embodiment 2

[0072] There are many differences between the present embodiment and embodiment 1, and the specific steps of the culture method of alum root " tiramisu " in the present embodiment are as follows:

[0073] (1) Aseptic system establishment: Select a mother plant that grows robustly and is free of diseases and insect pests, and place it in a greenhouse for cultivation. Spray 800 times of carbendazim 7 days before collecting materials; when collecting materials, choose to cut newborn petioles that are 35 days old, rinse them with running water for 10 minutes, and let them dry Dry; wipe the petiole with cotton containing 75% alcohol, wipe off the epidermis on the petiole, be careful not to break the petiole when wiping, and wash it twice with sterile water; put the material into 0.1% mercuric chloride solution for 7 minutes, no Bacteria water was washed 5 times, and the petioles were cut into 1.5 cm sections to obtain sterilized explants by shaking for 3 minutes each time.

[0074]...

Embodiment 3

[0080] The present embodiment and embodiment 1, embodiment 2 all have more difference, in the present embodiment to the specific steps of alum root " tiramisu " cultivation method are as follows:

[0081] (1) Establishment of sterile system: Select a mother plant that grows robustly and is free of diseases and insect pests, and place it in the greenhouse for cultivation. Spray 1000 times of carbendazim 10 days before collecting materials; when collecting materials, choose to cut newborn petioles that are 40 days old, rinse them with running water for 10 minutes and let them dry Dry; wipe the petiole with cotton containing 75% alcohol, wipe off the epidermis on the petiole, be careful not to break the petiole when wiping, and wash it twice with sterile water; put the material into 0.1% mercuric chloride solution for disinfection for 8 minutes, without Bacteria water was washed 6 times, each time shaking for 4 minutes, and the petioles were cut into 1 cm segments to obtain steril...

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Abstract

The invention relates to a tissue culture method for rapid propagation of alum root "tiramisu", and relates to the technical field of plant tissue culture. The present invention takes alum root petiole as explant, involves steps such as aseptic system establishment, callus induction, callus induction to form adventitious buds, adventitious bud proliferation, rooting induction, seedling hardening and transplanting, etc., and establishes a complete set of Tissue culture rapid propagation method of alum root "Tiramisu". This culture method has the advantages of fast propagation speed, high multiplication rate and low pollution rate, and can quickly produce high-quality seedlings in the production process. Commercial development has important practical application value.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method for rapid propagation of alum root "tiramisu". Background technique [0002] Alum root "Tiramisu" (Heuchera micrantha), a plant of the genus Heuchera micrantha, a perennial perennial herb of the genus Heuchera micrantha, is cold-resistant and can tolerate low temperatures around minus 20 degrees. It prefers semi-shade environments and is not resistant to direct sunlight. , It has strong adaptability to various soils, and fertile and loose weakly acidic soil is suitable. The leaves of this variety are broad-hearted. In spring and summer, the leaf color is light green with inconspicuous white markings. In autumn and winter, the leaf color turns red along the veins from the inside to the outside until the whole leaf turns wine red. The appearance of the plant varies Seasonal rich hue changes, high ornamental value, deeply loved by gardening enth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林巧玲高丽张学超于运乐高睿琦辛培培
Owner 北京花乡花卉科技研究所有限公司
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