A kind of date palm field sampling treatment agent and preparation method and use method thereof

Abstract

The invention discloses a field sampling treatment agent for date palm, comprising reagent A and reagent B, wherein the reagent A includes 1000-1500 times dilution of azoxystrobin, 1000-1500 times dilution of difenoconazole and carbendazim 800-1000-fold dilution, reagent B includes 6-benzylaminoadenine solution with mass concentration of 5-15mg / L and isopentenyl adenine solution with mass concentration of 1-5mg / L; preparation method: (1) Weigh azoxystrobin, difenoconazole and carbendazim, then dilute with water respectively, and mix to obtain reagent A; (2) weigh 6-benzylaminoadenine and isopentenyladenine, then use respectively Dissolve the sodium hydroxide solution, dilute to volume with sterile water, and mix to obtain reagent B. The field sampling treatment agent for the date palm of the invention can significantly reduce the fungal contamination rate and the endophyte contamination rate, so that the explants can maintain high differentiation ability, and it is easier to carry out detoxification treatment in the process of tissue culture, improve cell differentiation ability, and promote explants. Implants induce callus and shorten the induction period.

Description

technical field [0001] The invention relates to the field of plant biotechnology, and more particularly to a field sampling treatment agent for date palm and a preparation method and use method thereof. Background technique [0002] Date palm is a perennial tropical palm plant, dioecious, and is an important woody food crop in Arab countries. Date palm can use seeds to reproduce offspring, but because it is a dioecious plant and is in a highly heterozygous state, this method of reproduction will lose the target traits of the parent in production, and cannot guarantee the excellent characteristics and fruit of the mother plant. feature. [0003] Although date palm tissue culture technology can not only maintain the excellent characteristics of the mother plant, but also improve the multiplication coefficient, realize the supply of seedlings, and can also be used for breeding and in vitro germplasm preservation. However, as a palm species, date palm has a slow growth cycle an...

AI Technical Summary

Problems solved by technology

Date palms can use seeds to reproduce offspring, but because it is a dioecious plant and is in a highly heterozygous state, this method of reproduction will lose the target traits of the parents in production, and cannot guarantee the excellent characteristics and fruit of the mother plant. feature

[0003] Although the date palm tissue culture technology can not only maintain the excellent traits of the mother plant, but also increase the multiplication coefficient, realize the supply of seedlings, and can also be used for breeding and in vitro germplasm preservation, but the date palm is a palm tree species, and the growth cycle is slow. The ability of cell division is relatively slow, the hardness of the cutin of the leaves is high, and the leaves are seriously laminated. Bacteria and fungi are easy to breed inside the tree. If no field treatment is carried out before sampling, the fungi inside the sample will grow rapidly after the sample is separated from the parent body. Even after the disinfectant layer After being transferred into the induction medium, endophyte contamination and fungal infection are also very easy to occur, and excessive use of disinfectants will also cause cell damage, reduced viability, and weakened differentiation ability of the explants after they are separated from the mother.

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