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A kind of induction culture method of white and embryoid body

A culture method and technology of embryoid bodies, applied in the field of inductive culture of white and embryoid bodies, to achieve the effect of solving the shortage of plant resources and seedling resources of white and

Active Publication Date: 2019-01-01
CHENGDU JIANJIANG PHARMA FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report that the protocorm embryogenic cells were first cultured from the seeds of Baiji, and then the embryoid bodies of Baiji were further induced from the protocorm embryogenic cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The induction culture method of the white and embryoid bodies provided by the present embodiment comprises the following steps:

[0017] (1) Selection and disinfection of seeds: select mature and freshly cracked seeds of Baiji, put the seeds of Baiji and 0.1% mercuric chloride solution in a volume ratio of 1:10 into a centrifuge tube for disinfection for 11 minutes, and then Wash with sterile water for 12 minutes;

[0018] (2) Induction of protocorms: Put the sterilized Baiji seeds into the semi-solid medium 1 / 2MS+6-BA0.5mg / L+activated carbon 4.0g / L+agar 2.0g / L with the medicine spoon, and use the medicine spoon along the Stir in the same direction to evenly disperse the seeds of Baiji in the middle and lower part of the semi-solid medium. The inoculation density is 2500 grains / L, and the protocorms are grown under the conditions of a culture temperature of 25°C, 16 hours of light per day, and a light intensity of 2000lx. The induction culture of cultured, cultivated 1...

Embodiment 2

[0023] The induction culture method of the white and embryoid bodies provided by the present embodiment comprises the following steps:

[0024] (1) Selection and disinfection of seeds: select ripe and freshly cracked seeds of Baiji, put the seeds of Baiji and 0.1% mercuric chloride solution in a centrifuge tube for disinfection according to the volume ratio of 1:10 for 7 minutes, and then Wash with sterile water for 8 minutes;

[0025] (2) Induction of protocorms: Put the sterilized Baiji seeds into the semi-solid medium 1 / 2MS+6-BA0.1mg / L+activated carbon 3.0g / L+agar 1.5g / L with the medicine spoon, and use the medicine spoon along the Stir in the same direction, so that the seeds of Bletilla chinensis are evenly dispersed in the middle and lower part of the semi-solid medium. The inoculation density is 2000 grains / L, and the protocorm is grown under the conditions of a culture temperature of 20°C, 10 hours of light per day, and a light intensity of 1500lx. The induction cultu...

Embodiment 3

[0030] The induction culture method of the white and embryoid bodies provided by the present embodiment comprises the following steps:

[0031] (1) Selection and disinfection of seeds: select ripe and freshly cracked seeds of Baiji, put the seeds and 0.1% mercuric chloride solution in a centrifuge tube at a volume ratio of 1:10 for disinfection for 15 minutes, and then Wash with sterile water for 15 minutes;

[0032] (2) Induction of protocorms: Put the sterilized Baiji seeds into the semi-solid medium 1 / 2MS+6-BA1.0mg / L+activated carbon 5.0g / L+agar 3.0g / L with the medicine spoon, and use the medicine spoon along the Stir in the same direction to evenly disperse the seeds of Baiji in the middle and lower part of the semi-solid medium. The inoculation density is 3000 grains / L, and the protocorms are grown under the conditions of a culture temperature of 26°C, 24 hours of light per day, and a light intensity of 3000lx. Induction culture, cultured for 15 days statistics, the infe...

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PUM

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Abstract

The invention discloses an induction culture method of a rhizoma bletillae embryoid. The induction culture method comprises the following steps: taking a rhizoma bletillae seed as an explant, culturing a protocorm without differentiating cotyledons firstly, then carrying out culturing on the protocorm without differentiating cotyledons to obtain embryogenic tissue cells with strong division rate and green color, and carrying out induction culture on the embryogenic tissue cells to obtain the rhizoma bletillae embryoid finally. The induction culture method provides a new way for mass cultivation and rapid reproduction of good quality seedlings of rhizoma bletillae, and the problem that the rhizoma bletillae plant resources and seedling resources are short at present can be effectively solved.

Description

technical field [0001] The invention relates to a method for inducing embryoid bodies of Rhizoma saponiosa, in particular to a method for inducing and culturing embryoid bodies of Rhizoma saponiosa. Background technique [0002] The Chinese Pharmacopoeia stipulates that the traditional Chinese medicine Baihe is the dried tuber of Orchidaceae Baiji and Bletilla striata (Thunb.) Reichb.f., which has the functions of nourishing the lungs, reducing swelling, promoting muscle growth, stopping bleeding, and suppressing sores. It is used to treat lung injuries and coughs Blood, gold sore bleeding, ulcer pain, soup fire burns, chapped hands and feet embolism. [0003] In recent years, due to its good effect in treating hemostasis, reducing swelling and promoting muscle growth, and as a raw material for cosmetics, the demand has increased greatly. Since the 1980s, Baiji has become one of the most representative and globally influential medicinal plant resources among medicinal plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 王跃华陈燕黄鹏杨维维唐金容庄钰金刘洪明王宗兵
Owner CHENGDU JIANJIANG PHARMA FACTORY
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