A kind of induction culture method of white and embryoid body

Abstract

The invention discloses an induction culture method of a rhizoma bletillae embryoid. The induction culture method comprises the following steps: taking a rhizoma bletillae seed as an explant, culturing a protocorm without differentiating cotyledons firstly, then carrying out culturing on the protocorm without differentiating cotyledons to obtain embryogenic tissue cells with strong division rate and green color, and carrying out induction culture on the embryogenic tissue cells to obtain the rhizoma bletillae embryoid finally. The induction culture method provides a new way for mass cultivation and rapid reproduction of good quality seedlings of rhizoma bletillae, and the problem that the rhizoma bletillae plant resources and seedling resources are short at present can be effectively solved.

Description

technical field

[0001] The invention relates to a method for inducing embryoid bodies of Rhizoma saponiosa, in particular to a method for inducing and culturing embryoid bodies of Rhizoma saponiosa. Background technique

[0002] The Chinese Pharmacopoeia stipulates that the traditional Chinese medicine Baihe is the dried tuber of Orchidaceae Baiji and Bletilla striata (Thunb.) Reichb.f., which has the functions of nourishing the lungs, reducing swelling, promoting muscle growth, stopping bleeding, and suppressing sores. It is used to treat lung injuries and coughs Blood, gold sore bleeding, ulcer pain, soup fire burns, chapped hands and feet embolism.

[0003] In recent years, due to its good effect in treating hemostasis, reducing swelling and promoting muscle growth, and as a raw material for cosmetics, the demand has increased greatly. Since the 1980s, Baiji has become one of the most representative and globally influential medicinal plant resources among medicinal plant...