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A method for in vitro rapid propagation of rare and endangered plant Ilex mongolica

A technology of holly and plant, which is applied to the field of rapid propagation in vitro of rare and endangered plant holly mongolica, can solve the problems of difficulty in differentiation, induced browning of callus, etc., and achieves the effect of shortening time and alleviating the phenomenon of browning.

Active Publication Date: 2019-01-25
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for in vitro rapid propagation of the rare and endangered plant Ilex mongolica, to solve the problems of severe browning and difficult differentiation in callus induction in existing methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Obtainment of explants of P. mongolica: Inoculate the seeds of P. mongolica that have been sterilized by conventional methods into MS medium containing 2.0 mg / L 6-BA. After culturing in the dark for 7-11 days, the germinated seedlings are taken. , the thickness of the epicotyl is 3-5mm, keep the meristematic part of the cotyledon 2-5mm near the hypocotyl and the epicotyl 2-4mm, cut off the rest, and then cut longitudinally along the hypocotyl in the middle of the two cotyledons, The axillary buds were removed, and 2-4 wounds were cut at the junction of the cotyledons and the hypocotyls with a scalpel to obtain two cotyledonary node explants.

[0037] (2) Induction culture of clump buds: place the adaxial surface of the cotyledon node upward, and insert it into B containing 1.0 mg / L 6-BA at an inclination of 45 °C. 5 In the medium, the cells were induced in the dark for 5-7 days, the culture temperature was 22-26 °C during the day and 17-21 °C at night, and then indu...

Embodiment 2

[0042] The difference from Example 1 is that in the step (2), the induction culture of the clump buds is assisted by light induction for 20 days.

Embodiment 3

[0044] The difference from Example 1 is that in the elongation culture of step (3) clump buds, the auxiliary light is induced for 20d.

[0045] Insert the adaxial side of the cotyledon node upward, inclined at 45°C, and insert the B containing 1.0 mg / L 6-BA. 5 In the medium, the cells were induced in the dark for 5-7 days, the culture temperature was 22-26 °C during the day and 17-21 °C at night, and then the induction rate of artificial cluster buds was 78.3%, and the average number of buds was 2.6.

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Abstract

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for rapid propagation in vitro of a rare and endangered plant mongolica mongolica. Background technique [0002] Mongolian sand holly, also known as Dasha holly, belongs to the genus Sand holly of the leguminous family. Mainly distributed in the desert areas of Inner Mongolia, Gansu, Ningxia and other places. It is extremely adaptable to environmental conditions. It is the only strong xerophytic evergreen broad-leaved shrub in the northern desert region of my country. It is also a unique building plant in the Alxa desert region. Balance plays an important role, with significant economic significance and resource value. However, due to the narrow distribution range and the destruction of human activities in recent years, the number of Mongolian sand holly is decreasing, and it is now on the verge of extinction, and is listed as a national tertiary protected plant. Mon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 郭琪王沛雅杨晖杨涛张军李鑫郑群
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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