Culture method and application of high-yield cold-resistant stylosanthes guianensis
A cultivation method and technology of stylo, which is applied in the cultivation field of high-yield and cold-resistant stylo, can solve problems affecting the popularization and application of stylo, achieve the effects of enhancing growth toughness, promoting absorption capacity, and increasing growth speed
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Embodiment 1
[0031] A kind of cultivation method of high-yielding cold-resistant stylo
[0032] 1) Select Stylophyllum seeds as induction breeding material, use 45Hz low-frequency current to pretreat Stylophyllum seeds, treat 3 times, with an interval of 26min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:3 Mixed with water to form a mixed bacterial solution with a concentration of 17%;
[0033] The live bacteria content of the Trichoderma reesei is ≥ 2 × 10 10 cfu / g, preferably 2×10 10 ~3×10 10 cfu / g;
[0034] The live bacterial content of the described streptomyces convolutum is ≥ 4 × 10 10 cfu / g, preferably 4×10 10 ~6×10 10 cfu / g;
[0035] 2) Germination cultivation: sow 30-40 stylosperm seeds in a 50cm×50cm×10cm water content of 60-70% of the seedling substrate, and place them in the greenhouse for seedling cultivation. The temperature under the light is set to be 25°C in the greenhouse. The temperature in the dark is 18°C;
[0036] Dur...
Embodiment 2
[0043] A kind of cultivation method of high-yielding cold-resistant stylo
[0044] 1) Select the Stylophyllum seeds as the induction breeding material, use 40Hz low-frequency current to pretreat the Stylophyllum seeds, treat them 4 times with an interval of 20min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:3 Mixed with water to form a mixed bacterial solution with a concentration of 15%;
[0045]2) Germination culture: sow 30 to 40 stylosa seeds in a 50cm×50cm×10cm water tank with 60 to 70% water.
[0046] In the seedling raising medium, place the seedlings in a greenhouse, set the temperature in the greenhouse to be 32°C under the light, and 20°C under the dark;
[0047] Watering once every 3 days during seedling raising, and watering at 11:00 in the morning, the temperature of the water is 10°C, after watering once, use 7000lux light to irradiate the Stylophyllum seedlings with heat source for 3 days, and irradiate for 4 hours / d; ...
Embodiment 3
[0053] A kind of cultivation method of high-yielding cold-resistant stylo
[0054] 1) Select Stylophyllum seeds as the induction breeding material, use 50Hz low-frequency current to pretreat the Stylophyllum seeds, treat 3 times with an interval of 20min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:4 Mixed with water to form a mixed bacterial solution with a concentration of 20%;
[0055] 2) Germination culture: sow 30 to 40 stylosperm seeds in a 50cm x 50cm x 10cm seedling medium with 60 to 70% moisture, set the temperature in the greenhouse to 37°C under light and 20°C in darkness. ℃;
[0056] Watering once every 4 days during the seedling raising period, and watering at 12:00 in the morning, the temperature of the water is 15°C, after the watering once, use the light of 10000lux intensity to irradiate the Stylophyllum seedlings with a heat source for 2 days, and irradiate for 2 hours / d;
[0057] The mass ratio of red sandy soil, h...
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