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Culture method and application of high-yield cold-resistant stylosanthes guianensis

A cultivation method and technology of stylo, which is applied in the cultivation field of high-yield and cold-resistant stylo, can solve problems affecting the popularization and application of stylo, achieve the effects of enhancing growth toughness, promoting absorption capacity, and increasing growth speed

Pending Publication Date: 2022-03-25
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Stylo has become the main grass species in the tropical and subtropical regions of South China. However, it is difficult for the high-yield and high-quality Stylo varieties commonly used in production today to survive the winter in the central and northern parts of South China, which greatly affects the large-scale promotion and application of Stylo. It has become the primary issue in the production and popularization of Stylophyllum in South China

Method used

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  • Culture method and application of high-yield cold-resistant stylosanthes guianensis
  • Culture method and application of high-yield cold-resistant stylosanthes guianensis
  • Culture method and application of high-yield cold-resistant stylosanthes guianensis

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Experimental program
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Effect test

Embodiment 1

[0031] A kind of cultivation method of high-yielding cold-resistant stylo

[0032] 1) Select Stylophyllum seeds as induction breeding material, use 45Hz low-frequency current to pretreat Stylophyllum seeds, treat 3 times, with an interval of 26min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:3 Mixed with water to form a mixed bacterial solution with a concentration of 17%;

[0033] The live bacteria content of the Trichoderma reesei is ≥ 2 × 10 10 cfu / g, preferably 2×10 10 ~3×10 10 cfu / g;

[0034] The live bacterial content of the described streptomyces convolutum is ≥ 4 × 10 10 cfu / g, preferably 4×10 10 ~6×10 10 cfu / g;

[0035] 2) Germination cultivation: sow 30-40 stylosperm seeds in a 50cm×50cm×10cm water content of 60-70% of the seedling substrate, and place them in the greenhouse for seedling cultivation. The temperature under the light is set to be 25°C in the greenhouse. The temperature in the dark is 18°C;

[0036] Dur...

Embodiment 2

[0043] A kind of cultivation method of high-yielding cold-resistant stylo

[0044] 1) Select the Stylophyllum seeds as the induction breeding material, use 40Hz low-frequency current to pretreat the Stylophyllum seeds, treat them 4 times with an interval of 20min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:3 Mixed with water to form a mixed bacterial solution with a concentration of 15%;

[0045]2) Germination culture: sow 30 to 40 stylosa seeds in a 50cm×50cm×10cm water tank with 60 to 70% water.

[0046] In the seedling raising medium, place the seedlings in a greenhouse, set the temperature in the greenhouse to be 32°C under the light, and 20°C under the dark;

[0047] Watering once every 3 days during seedling raising, and watering at 11:00 in the morning, the temperature of the water is 10°C, after watering once, use 7000lux light to irradiate the Stylophyllum seedlings with heat source for 3 days, and irradiate for 4 hours / d; ...

Embodiment 3

[0053] A kind of cultivation method of high-yielding cold-resistant stylo

[0054] 1) Select Stylophyllum seeds as the induction breeding material, use 50Hz low-frequency current to pretreat the Stylophyllum seeds, treat 3 times with an interval of 20min, and then soak them in Trichoderma reesei and Streptomyces clumps at a mass ratio of 1:4 Mixed with water to form a mixed bacterial solution with a concentration of 20%;

[0055] 2) Germination culture: sow 30 to 40 stylosperm seeds in a 50cm x 50cm x 10cm seedling medium with 60 to 70% moisture, set the temperature in the greenhouse to 37°C under light and 20°C in darkness. ℃;

[0056] Watering once every 4 days during the seedling raising period, and watering at 12:00 in the morning, the temperature of the water is 15°C, after the watering once, use the light of 10000lux intensity to irradiate the Stylophyllum seedlings with a heat source for 2 days, and irradiate for 2 hours / d;

[0057] The mass ratio of red sandy soil, h...

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Abstract

The invention belongs to the technical field of plant induced breeding, and particularly relates to a high-yield cold-resistant stylosanthes guianensis culture method and application thereof, and the method comprises the following steps: 1) selecting stylosanthes guianensis seeds as an induced breeding material, and pretreating the seeds; 2) germination culture: sowing stylosanthes guianensis seeds in a seedling culture medium, placing the seedling culture medium in a greenhouse for seedling culture, watering once every 3-4 days during seedling culture, controlling the temperature of the water to be 10-15 DEG C, and performing heat source irradiation after watering; 3) rooting culture: obtaining seedlings after germination, spraying a rooting solution A on the surface of the seedling culture substrate, transferring the seedling culture substrate to a rooting pot for rooting culture, and spraying a rooting solution B after 3-5 days; and 4) growth culture: after the stylosanthes guianensis seedlings grow to 90-110cm and 3-4 days later, spraying a growth solution once, the stylosanthes guianensis with strong growth ability and excellent cold resistance is cultured by the method, the method can be popularized to the southern subtropical region, and the culture method is provided for expanding the utilization range of the stylosanthes guianensis.

Description

technical field [0001] The invention belongs to the technical field of plant induction and breeding, and in particular relates to a method for cultivating high-yield cold-resistant stylo and its application. Background technique [0002] Stylo has become the main grass species in the tropical and subtropical regions of South China. However, it is difficult for the high-yield and high-quality Stylo varieties commonly used in production today to survive the winter in the central and northern parts of South China, which greatly affects the large-scale promotion and application of Stylo. It has become the primary issue in the production and popularization of Stylophyllum in South China. In the central and northern part of South China, most tropical perennial legumes are difficult to survive the winter, and most temperate perennial legumes are difficult to survive summer. Therefore, the purpose of the present invention is to breed high-quality grasses with strong cold resistance ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01G24/10A01G24/20A01C1/00A01G7/04C05G3/00C05G5/20A01N43/38A01N43/90A01N59/24A01N59/00A01N37/10A01N37/40A01N65/44A01P21/00
CPCA01G22/00A01G24/10A01G24/20A01C1/00A01G7/04C05F11/00C05G3/00C05G5/20A01N43/38A01N43/90A01N59/24A01N59/00A01N37/10A01N37/40A01N65/44A01N65/00C05F11/10
Inventor 丁迪云张庆智陈卫东孙浩吴林瑛
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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