43 results about "Stylosanthes guianensis" patented technology

The invention discloses a bacterial strain of a root nodule nitrogen-fixing strain series RY3 and application thereof. The bacterial strain (Bradyrhizobium sp. RY3) is preserved in the China Center for Type Culture Collection on November 13th, 2009, and the collection number of the bacterial strain is CCTCC No: M 209267. The root nodule nitrogen-fixing strain series RY3 of the invention is grafted back to Stylosanthes guianensis TPRC2 and TPRC5 so that the fresh weight and dry weight of the Stylosanthes guianensis are increased by over triple. The RY3 is a highly-efficient bacterial strain which can ensure that the high yield of both the fresh weight and dry weight of the Stylosanthes guianensis can be achieved and the dissemination capacity of the Stylosanthes guianensis is stronger after the Stylosanthes guianensis is grafted with rhizobia; and after being released to the natural environment, the RY3 has no harm to human, animals and plants and does not pollute the environment.

The invention discloses a Bradyrhizobium sp.RY4 strain and application thereof. The Bradyrhizobium sp.RY4 strain is preserved at the China center for type culture collection and the preservation number is CCTCC NO:M209268. When the Bradyrhizobium sp.RY4 is back-jointed to stylosanthes guianensis TPRC2 and TPRC5, the number of root nodules, the dry weight of the root nodule, the height of the strain, the fresh weight of the plant and the nitrogenase activity are greatly increased and obviously higher than those in a control group; and the effect of the RY4 back jointed to the stylosanthes guianensis TPRC2 is better. Thalli are released to the natural environment, the thalli increase the number of the groups of nodule bacteria in soil, contribute to the nodulation and nitrogen fixation of the stylosanthes guianensis, are harmless for human beings, animals and plants, and do not pollute the environment.

The invention discloses a RY2 strain of a root nodule nitrogen-fixing bacterial strain system and application thereof. The strain (Bradyrhizobium yuanmingense RY2) is preserved in the China Center for Type Culture Collection (CCTCC) on 13th November in 2009. The preservation number is CCTCC NO:M 209266. The RY2 strain of the root module nitrogen-fixing bacterial strain system can exclusively greatly improve the nitrogenase activity of TPRC2, so that the nitrogenase activity of the TPRC2 is over three times higher than that of TPRC5 and can achieve higher nitrogenase activity by using a few root nodules. The yield of stylosanthes guianensis can be improved by over 30 to 40 percent by inoculating the root nodule bacteria, and the root nodule bacteria can be suitable for intercropping of stylosanthes guianensis TPRC2 and provides a large number of nitrogen for the stylosanthes guianensis, crops and soil. Meanwhile, the RY2 has strong salt tolerance, can grow in the environment that the NaCl concentration is higher than 0.25mol/L, can be planted in the salinized soil and has better effects of improving the yield of the stylosanthes guianensis and utilizing and improving the salinized soil.

The invention discloses a degraded soil improving method with stylosanthes guianensis as ecological forage. The JinshaRiver dry-hot valley degraded soil is chosen to be treated with the method. According to the method, thoroughly decomposed farmyard manure and calcium superphosphate are applied during soil preparation, stylosanthes guianensis seedlings are transplanted in the rainy season, urea is further applied for one to two times when the stylosanthes guianensis seedlings germinate, timely drainage is guaranteed in the rainy season and the soil water content is limited below 15%, timely watering is guaranteed in the arid season and the soil water content is kept above 3%, 50% carbendazim wettable powder is used every year to prevent the occurrence of anthracnose, and 20% acetamiprid powder 3000 times liquid is used to kill aphids or armyworms in a spraying mode. According to the ecological characteristic of the Jinsha River dry-hot valley degraded soil, the method fully utilizes characteristics, such as drought-enduring, growing fast, cutting-enduring, strong nitrogen fixing capacity, strong adaptability and extensive management, of the stylosanthes guianensis, soil organic matter, the total nitrogen and available phosphorus content, and soil water contents in soil layers of 0-20 cm and 20-40 cm in the arid season are greatly improved.

The invention discloses a fertilizer rich in organic potassium and a preparation method thereof. The fertilizer rich in organic potassium consists of the following components in parts by weight: 14-18 parts of potassium sulfate, 0.7-1.8 parts of ferrous sulfate, 6-9 parts of ammonium sulfate, 13-15 parts of diammonium phosphate, 0.7-1.8 parts of borax, 5-8 parts of bentonite, 4-7 parts of diatomite, 5-8 parts of high-calcium clay, 3-6 parts of zeolite, 4-6 parts of fulvic acid, 20-25 parts of plant straw, 4-7 parts of trace element, 1-3 parts of fenaminosulf and 2-4 parts of chlorothalonil. Since plants highly rich in potassium including celosia argentea, pokeberry root, rudbeckia laciniata, stylosanthes guianensis and amaranthus cruentus are adopted in the fertilizer, not only the active ingredients of the fertilizer but also the content of organic potassium in the fertilizer are increased.

The invention relates to the technical field of planting, in particular to a method for interplanting cassia alata Linn and stylosanthes guianensis. According to the method, cassia alata Linn and stylosanthes guianensis are interplanted, and the super-strong cadmium-rich capability of the stylosanthes guianensis is utilized to achieve the purpose of improving the cadmium content in soil. In addition, the super-strong nitrogen fixing capacity of the cassia alata Linn is also utilized to provide enough nitrogen element for the soil, and growth and commensalism of stylosanthes guianensis and cassia alata Linn is ensured. Cassia alata Linn branches and stylosanthes guianensis seeds are preprocessed, the germination rate of the cassia alata Linn and the rooting rate of the stylosanthes guianensis can be greatly improved, growth of the cassia alata Linn and stylosanthes guianensis is effectively promoted, commensalism of stylosanthes guianensis and cassia alata Linn is achieved, accordinglysoil fertility supplementation is accelerated, and thus the purpose of improving the cadmium content in soil is achieved.

The invention discloses a root nodule azotobacter strain RY1 bacterial strain and an application thereof. The bacterial strain (Bradyhizobium sp.RY1) is obtained from gathering fresh root nodules in main Stylosanthes guianensis planting areas in China and separating and purifying in a laboratory, the partial sequence of 16S rDNA of the bacterial strain is measured, and the bacterial strain is determined to be a new bacterial strain of Bradyrhizobium by the phylogenesis analysis and is named as Re Yan 1 (RY1). By the potted planting and the inoculation test in the laboratory, the nodule azotobacter strain RY1 bacterial strain is proven to have the characteristic of enabling the two main Stylosanthes guianensis varieties (TPRC2 and TPRC5) in China to realize the high-efficiency nodulation and achieve the high yield. Compared with the inoculation-free process, the inoculation with the root nodule can improve the yield of the Stylosanthes guianensis by more than 2 times, and can be used as one of conventional Stylosanthes guianensis culturing measures for planting the Stylosanthes guianensis.

The invention discloses a preparation method of a stylosanthes guianensis biological feed. The stylosanthes guianensis biological feed is prepared by the following steps of: chopping stylosanthes guianensis; adding sugar and third-grade seed liquid of lactobacillus plantarum; uniformly mixing; and exhausting, sealing and storing. The preparation method has a simple process, takes the stylosanthes guianensis as a major raw material, has a wide raw material source and low cost. An acid environment is created through the fermentation of lactobacilli, so that harmful microorganisms are suppressed. The stylosanthes guianensis is stored and the nutrition of the stylosanthes guianensis is kept effectively. The biological feed has high cellulose content, fresh scent and high palatability and digestibility and is a high-quality animal feed.

The invention discloses a gene SgME1 which codes malic enzyme as well as an application of the gene SgME1. The nucleotide sequence of the malic enzyme gene SgME1 is shown as SEQIDNO:1, and the amino acid sequence of coded proteins is shown as SEQIDNO:2. The SgME1 gene has the functions of regulating and controlling synthesis of malic acid of stylosanthes guianensis, and over-expressing the SgME1 to accelerate synthesis of malic acid of yeasts, hairy roots of beans and Arabidopsis and increasing the aluminum tolerance of the SgME1.

The present invention discloses a cultivation method for interplanting stylosanthes guianensis and annual ryegrass, and belongs to the technical field of herbage production. The method comprises the steps of rational close planting, stylosanthes guianensis field management, stylosanthes guianensis mowing utilization, ryegrass interplanting, ryegrass field management, ryegrass mowing utilization and ryegrass multiple cropping. According to the method, after decomposed farm manure and calcium superphosphate are applied to arable land of Jinsha River dry-hot valley, field planting ponds are dug to plant the stylosanthes guianensis; the stylosanthes guianensis field management comprises the steps of topdressing, draining flooded fields and irrigating, weeding and controlling insect diseases; and topdressing the stylosanthes guianensis after every mowing, and interplanting ryegrass seeds from the end of September to October after mowing the stylosanthes guianensis in the first year. According to the method, the defect of greenfeed shortages in the autumn and winter of the Jinsha River dry-hot valley is overcome, a new raw material production method is provided for development of winter animal husbandry of the Jinsha River dry-hot valley, waste of water resources, production cost and land resources is reduced, and compared with a method of only planting the stylosanthes guianensis, the benefit per mu can be increased by 338-959 yuan.

The invention discloses a potassium-rich microcapsule fertilizer and a preparation method thereof. The organic-potassium-rich fertilizer is composed of the following components in parts by weight: 14-18 parts of potassium sulfate, 0.7-1.8 parts of ferrous sulfate, 6-9 parts of ammonium sulfate, 13-15 parts of diammonium phosphate, 0.7-1.8 parts of borax, 5-8 parts of bentonite, 4-7 parts of kieselguhr, 5-8 parts of high-calcium clay, 3-6 parts of zeolite, 4-6 parts of fulvic acid, 20-25 parts of plant straw, 4-7 parts of microelement and the like. Due to the adoption of potassium-rich plants, including feather cockscomb, Phytolacca acinosa, coneflower, stylosanthes guianensis and Grain Amaranth, the organic components of the fertilizer are increased, and the organic potassium content of the fertilizer is enhanced.

The invention discloses a potassium-rich traditional Chinese medicine fertilizer and a preparation method thereof. The organic-potassium-rich fertilizer is composed of the following components in parts by weight: 14-18 parts of potassium sulfate, 0.7-1.8 parts of ferrous sulfate, 6-9 parts of ammonium sulfate, 13-15 parts of diammonium phosphate, 0.7-1.8 parts of borax, 5-8 parts of bentonite, 4-7 parts of kieselguhr, 5-8 parts of high-calcium clay, 3-6 parts of zeolite, 4-6 parts of fulvic acid, 20-25 parts of plant straw, 4-7 parts of microelement and the like. Due to the adoption of potassium-rich plants, including feather cockscomb, Phytolacca acinosa, coneflower, stylosanthes guianensis and Grain Amaranth, the organic components of the fertilizer are increased, and the organic potassium content of the fertilizer is enhanced.

The invention discloses a method for improving southern red soil. The method comprises: 1, taking a soil sample and determining soil index values T such as soil pH, organic matter content and nutrientcontent, 2, determining a final value Z, a soil index buffer value H, a soil index value T and soil layer soil weight W of the red soil to be improved, wherein W is obtained through a formula of W=improved soil thickness*667*1.1/1000, 3, determining the amount F of elements to be used according to the formula of F=(Z-T)*H*W, 4, determining the material for soil improvement and improving the soil,and 5, planting Stylosanthes guianensis in the improved soil and carrying out green manure dressing.

The invention relates to the technical field of feed processing, in particular to anthocephalus chinensis leaf powder serving as a silage additive and application thereof in stylosanthes guianensis silage. According to the invention, the anthocephalus chinensis leaf powder is used as a silage additive, is added into stylosanthes guianensis silage, reduces the water content of the stylosanthes guianensis silage, remarkably reduces the pH value, propionic acid content, non-protein nitrogen content and ammoniacal nitrogen content of the silage, remarkably increases the number of lactic acid bacteria, lactic acid content, crude protein content and true protein content, and has a remarkable improvement effect on the improvement of the quality of the stylosanthes guianensis silage and on the protection of nutritional ingredients; the prepared stylosanthes guianensis silage can maintain the nutrition of stylosanthes guianensis, is rich in nutrition, mellow in smell, soft, juicy and good in palatability, and the quality of the stylosanthes guianensis silage is improved; the anthocephalus chinensis leaf powder is cheap, easy to obtain and simple and convenient to operate.

The invention relates to an interplanting method of blueberries and Stylosanthes guianensis and belongs to the technical field of agricultural planting. The method includes the steps of firstly, selecting seeds, to be more specific, screening out good blueberry and Stylosanthes guianensis seeds; secondly, selecting land and preparing soil; thirdly, transplanting and planting, to be more specific,planting the good blueberry seeds screened out in the first step on planting rows prepared in the second step in spring; planting the Stylosanthes guianensis on two sides of the blueberries in May; fourthly, performing field management, to be more specific, performing weeding, irrigation, pruning, fertilizing, disease and pest control, cutting and seedling supplementing on the planted blueberriesand Stylosanthes guianensis. The method has the advantages that interplanting is utilized, weeds can be covered and inhibited, humus is increased, nitrogen fixation is performed to increase soil fertility, soil temperature is regulated, and accordingly the growth of the blueberries in high-temperature regions is improved.

The invention discloses a feeding method capable of converting phytol into phytanic acid in a higher proportion in ruminant rumen. The feeding method comprises the following steps: S1, applying a high nitrogen fertilizer in a cultivation process; S2, silage preparation of forage: preparing silage from the collected forage; S3, preparation of feed: coarse feed and fine feed are matched, the matching ratio of the coarse feed to the fine feed in the feed is 40: 60, the coarse feed is composed of various forage, and comprises king grass, cassava stems and leaves, stylosanthes guianensis, broussonetia papyrifera leaves, pineapple peel and weeds; and S4, forage feeding: feeding the ruminants in a free feeding mode, more phytol molecules in the daily ration are converted into phytanic acid molecules under the action of rumen biological hydrogenation by improving the feeding method, the phytol molecules are finally converted into animal products through metabolism, and finally the technical requirement for producing high-quality animal products is met.

The invention discloses a special chemical fertilizer for raspberry and a preparation method of the special chemical fertilizer. The special chemical fertilizer is prepared from the following formula components in parts by mass: 28-30 parts of corn straw, 23-25 parts of wheat straw, 15-17 parts of sesame straw, 10-12 parts of dried stylosanthes guianensis, 7-9 parts of crop straw decomposer, 8-10 parts of yeast fertilizer, 15-17 parts of ammonium sulfate, 12-14 parts of monopotassium phosphate, 9-11 parts of phosphate rock powder, 6-8 parts of chelate zinc, 3-5 parts of molybdenum disulfide, 2-4 parts of tetramethyl glutaric acid and 60-80 parts of water. The special chemical fertilizer for the raspberry contains rich nutrient substances and is capable of promoting the raspberry fruits to be bright red, smooth and attractive, and palatably sweet and sour; and the special chemical fertilizer contains the tetramethyl glutaric acid which is capable of accelerating raspberry rooting, promoting the fruits to grow and reducing falling flowers and fruits.

The invention relates to antibiotic-free beef cattle stylosanthes guianensis silage as well as a preparation method and application thereof. The stylosanthes guianensis silage comprises the following components in parts by weight: 940-970 parts of stylosanthes guianensis, 9-20 parts of rice hull powder, 10-20 parts of corn bran, 1-2 parts of probiotics, 6-10 parts of Chinese herbal medicines and 5-10 parts of a chenopodium glaucum extract; wherein the Chinese herbal medicines comprise scutellaria baicalensis, red-knees herb and perfoliote knotweed herb, and the mass ratio of the scutellaria baicalensis to the red-knees herb to the perfoliote knotweed herb is (5-10) : (2-8) : (4-10); the stylosanthes guianensis silage further comprises a protein degradation inhibitor, and the mass of the protein degradation inhibitor is 0.3%-1.2% of the mass of stylosanthes guianensis. Compared with the prior art, the beef cattle feed has the advantages that stylosanthes guianensis is used as a main raw material, a small amount of rice hull powder, corn bran, probiotics, Chinese herbal medicines and the chenopodium glaucum extract are used as auxiliary materials, and radix scutellariae, red-knees herb, perfoliote knotweed herb, the chenopodium glaucum extract and other components are added into the feed, so that the production performance, immunity and oxidation resistance of beef cattle can be improved, the nutritional value and palatability of the feed can be improved, and the utilization rate of stylosanthes guianensis resources can be increased.

The invention relates to the field of fertilizers, in particular to a fertilizer special for raspberries. The fertilizer is prepared from, by weight, 10-20 parts of sawdust, 10-12 parts of expanded perlite powder, 1-2 parts of nitrification inhibitor DMPP, 0.3-0.5 part of tea saponin, 28-30 parts of corn straw, 23-25 parts of wheat straw, 15-17 parts of sesame straw, 10-12 parts of dry stylosanthes guianensis, 7-9 parts of crop straw decomposition agent, 8-10 parts of yeast fertilizer, 15-17 parts of ammonium sulfate, 12-14 parts of mono-potassium phosphate, 9-11 parts of ground phosphate rock, 6-8 parts of chelate zinc, 3-5 parts of molybdenum disulfide, 2-4 parts of tetramethyl glutaric aciduria and 60-80 parts of water. The fertilizer special for the raspberries is rich in nutrient and can make raspberry fruits bright red and bright, glossy, attractive and proper in acidity and sweetness, and the formula includes the tetramethyl glutaric aciduria, so that the rooting speed of the raspberries is accelerated, the growth of the fruits are promoted, and the flower and fruit abscission is reduced.

The invention provides a fermented dietary supplement for animals. The dietary supplement is prepared from the following components: pennisetum hydridum, elephant grass, ryegrass, alfalfa, Rumex, grain amaranth, endives, Gaodan grass, prairie milk vetch, sweet sorghum, red clover, white clover, silphium perfoliatum, ixeris denticulata, Chinese pennisetum, wheat grass, sweet clover, barley grass, timothy hay, weeping alkaligrass, oat grass, agropyron cristatum, sainfoin, caragana microphylla, stylosanthes guianensis, awnless brome, lyme grass, siberian wildrye, feed corn, purslane herb, epimedium herb, ammophila arenaria, plantain herb, evening primrose, melinis minutiflora, achnatherum splendens, buckwheat, Chinese milkvetch, Japanese lawngrass, millet, green foxtail, peas, barnyard millet, artemisia arenaria seeds, acacia mangium, glutinous rice, sudan grass, vervain, oilseed rape, soybeans and sunflower seeds. The additive provided by the invention ensures dietary balance of animals,is processed and manufactured by natural fermentation, does not have any injury to human bodies, animals, plants and natural environments, and needs not adopting special protective measures.

The invention discloses beef cattle feed containing fermented banana stalks and leaves for preventing and treating nephritic edema. The beef cattle feed is prepared from, by weight, 80-90 parts of banana stalks and leaves, 60-70 parts of peanut powder cakes, 5-6 parts of rice bran, 35-40 parts of Stylosanthes guianensis, 30-35 parts of pineapple peel, 5-10 parts of daylily, 20-25 parts of bean curd, 12-16 parts of jasmine flower, molasses, lactic acid bacteria, cellulase, papain and an appropriate amount of water. The beef cattle feed containing the fermented banana stalks and leaves for preventing and treating the nephritic edema has the advantages that the content of tannin and content of polyphenols in the banana stalks and leaves can be decreased through high temperature; the molasses and the rice bran are added to provide a sufficient carbon source, so that growth and propagation of the lactic acid bacteria are promoted, and the content of each of ammonia nitrogen, soluble carbohydrate, crude fibers and tannin is decreased effectively; the beef cattle feed is capable of improving intestinal functions of beef cattle, preventing and treating gastrointestinal tract diseases such as diarrhea and dyspepsia, enhancing immunity and reducing bacterial infection of kidneys so as to prevent and treat the nephritic edema.

The invention discloses a planting method for interplanting stylosanthes guianensis in an orange orchard. The planting method comprises the following steps of selecting a planting field, digging planting holes, planting orange trees, interplanting the stylosanthes guianensis, managing young trees, managing the stylosanthes guianensis, harvesting the stylosanthes guianensis, managing fruiting trees in the next year, and harvesting oranges. The heat-resistant, drought-enduring and low forage grass stylosanthes guianensis is interplanted between rows of the orange orchard, green ecological cultivation of the orange orchard and the stylosanthes guianensis is achieved, the stylosanthes guianensis can inhibit weed growth, the weeding cost of the orange orchard is reduced, investment of chemical agents, agricultural films and the like is reduced, stylosanthes guianensis plants are low, field management is not hindered, time and labor are saved, the orange trees and the stylosanthes guianensis grow without interfering with each other and promote each other, land resources are fully utilized through interplanting, production benefits are increased, the stylosanthes guianensis can be used for raising poultry and livestock after being mowed or are dried into hay and silage in the sun, and can also be returned to the field on site for fertilization and soil improvement, and multiple purposes are achieved.

The invention discloses a method for inducing hairy roots of stylosanthes guianensis. The method comprises the steps of selection and culture of agrobacterium rhizogenes, acquisition of stylosanthes guianensis explants, infection of the stylosanthes guianensis explants, induction of the hairy roots of the stylosanthes guianensis and hairy root degerming culture and propagation, and the hairy root induction rate reaches 58.3%. The method for inducing the hairy roots of the stylosanthes guianensis provides important technical support for molecular biology research of the stylosanthes guianensis, and has a great application prospect.

The invention discloses a cultivation method for improving the yield and quality of heteropogon contortus. The method includes the steps of heteropogon contortus and stylosanthes guianensis interplanting, scientific fertilization, and field management such as water management and disease and pest control. By adopting the method, the technical problems that heteropogon contortus growing in a dry-hot valley is low in yield and poor in quality and cannot develop into dominant pasture in the region are solved, and meanwhile the problems that when the yield is increased simply by applying nitrogenand phosphorus fertilizer, the effect is unsatisfactory, the fertilizer utilization rate is low, the cost is increased, land is hardened, and groundwater is seriously polluted are solved.

The invention discloses a special feed for canaries and a preparation method of the special feed. The special feed is composed of the following components in parts by mass according to a formula: 12-14 parts of panicled fameflowers, 26-28 parts of schefflera arboricola, 41-43 parts of sorghum, 11-13 parts of ginkgo leaves, 38-40 parts of pear tree leaves, 6-8 parts of kumquats, 30-32 parts of scindapsus aureus leaves, 17-19 parts of crickets, 40-42 parts of stylosanthes guianensis, 25-27 parts of coarse shell powder, 12-14 parts of olive oil and 305-315 parts of water. The special feed is rich in nutrient substances and capable of promoting the feather of the canaries to be smooth and bright in color; and due to the addition of the kumquat component in the formula, the special feed has digestion-helping and anti-inflammatory effects and is beneficial to the health growth of the canaries and capable of reducing the dead rate.

The invention discloses a method for reducing soil nutrient loss by interplanting stylosanthes guianensis in a tableland tea garden. Stylosanthes guianensis is interplanted in the tableland tea garden, has the characteristics of cutting resistance, nitrogen fixation, drought resistance, good adaptability, shrub structure and the like, can biologically intercept soil carried in runoffs formed afterrainfall and has the intercepting, seeping, retaining and utilizing effect on rainfall, the water and soil loss amount and nitrogen nutrient loss amount of the tableland tea garden can be decreased,the organic matter content of soil is increased, the organic matter of the planting soil is increased, the nitrogen content of the soil is increased, accordingly the applying amount of chemical nitrogen fertilizers is decreased, and the purposes of improving the physical and chemical properties of the soil and increasing economic benefits of the tableland tea garden are achieved.