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Improvement method for sugarcane stem tip culture

The technology of sugarcane stem and stem tip is applied in the improvement field of sugarcane stem tip culture, which can solve the problems of low survival rate, phenol pollution, affecting the market supply and supply speed of sugarcane virus-free seedlings, etc. Cell division ability, beneficial effect on survival rate

Inactive Publication Date: 2021-11-05
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although this technology has been widely used, there are still problems such as serious phenol pollution and low survival rate, which have seriously affected the market supply and supply speed of virus-free sugarcane seedlings.

Method used

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  • Improvement method for sugarcane stem tip culture
  • Improvement method for sugarcane stem tip culture
  • Improvement method for sugarcane stem tip culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1 A kind of improved method of sugarcane shoot tip culture

[0065] (1) The growth period is 4 months, and the stem tip of the sugarcane tail tip (specifically select the leaf primordium 2mm below the growth point) that grows healthy and strong without damage by diseases and insect pests is used as an explant, soaked in 75% alcohol for 25s, and then rinsed with water 3 times. The treated shoot tips were heat-shocked in warm water at 40°C for 2 minutes.

[0066] (2) Put the improved MS medium into the culture bottle, and put the massive citrus pulp in the second carrying part, and lay filter paper on the bottom and top of the citrus pulp respectively, and then pass the first carrying part and the second carrying part through Screw the thread to fix it, then place the heat-shocked shoot tip on the outer wall of the top surface of the first bearing part, and hang the shoot tip bearing device in the culture bottle through the hook body, seal it, and cultivate it...

Embodiment 2

[0070] Embodiment 2 A kind of improved method of sugarcane shoot tip culture

[0071] (1) The growth period is 5 months, and the stem tip of the sugarcane tail tip (specifically selected to cut the leaf primordium 2.5 mm below the growth point) that grows healthy and strong without damage by diseases and insect pests is used as an explant, soaked in 75% alcohol for 28 seconds, and then rinsed with clear water Rinse 4 times. The treated shoot tips were heat-shocked at 42°C for 2.5 minutes.

[0072] (2) Put the improved MS medium into the culture bottle, and put the massive citrus pulp in the second carrying part, and lay filter paper on the bottom and top of the citrus pulp respectively, and then pass the first carrying part and the second carrying part through Screw the thread to fix it, then place the heat-shocked shoot tip on the outer wall of the top surface of the first bearing part, and hang the shoot tip bearing device in the culture bottle through the hook body, seal i...

Embodiment 3

[0076] Embodiment 3 A kind of improved method of sugarcane shoot tip culture

[0077] (1) The growth period is 6 months, and the stem tip of the sugarcane tail tip (specifically selected to cut the leaf primordium 3mm below the growth point) that grows healthy and strong without damage by diseases and insect pests is used as an explant, soaked in 75% alcohol for 30s, and then rinsed with water 5 times. The treated shoot tips were heat-shocked at 45°C for 3 minutes.

[0078] (2) Put the improved MS medium into the culture bottle, and put the massive citrus pulp in the second carrying part, and lay filter paper on the bottom and top of the citrus pulp respectively, and then pass the first carrying part and the second carrying part through Screw the thread to fix it, then place the heat-shocked shoot tip on the outer wall of the top surface of the first bearing part, and hang the shoot tip bearing device in the culture bottle through the hook body, seal it, and cultivate it unde...

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Abstract

The invention discloses an improvement method for sugarcane stem tip culture, and belongs to the technical field of agricultural production. The improvement method comprises the following steps: a stem tip of a sugarcane tail tip is selected as an explant, heat shock treatment is carried out in sterile water at 40-45 DEG C, the explant is inoculated into an improved MS culture medium, and culturing is performed until a new lateral bud grows; wherein in the culture period, cold stimulation is carried out 1-3 times every day from the fifth day of inoculation, and the cold stimulation lasts for 10-18 days; and the improved MS culture medium comprises the following components: MS, 0.05-0.15 mg / L of thidiazuron, 0.1-0.25 mg / L of 6-BA and 20-30 g / L of cane sugar. According to the improvement method for sugarcane stem tip culture, the highest survival rate of the stem tips reaches 86.35%, the culture period is shortened, and the requirement of the market for a large number of sugarcane asexual propagation materials is met.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to an improved method for cultivating sugarcane shoot tips. Background technique [0002] Sugarcane is a plant of the grass family Saccharum, native to tropical and subtropical regions. Sugarcane is rich in sugar and water, and also contains various vitamins, fats, proteins, organic acids, calcium, iron and other substances that are very beneficial to human metabolism. It is mainly used for sugar production, and the annual market demand is relatively large. However, sugarcane is a clonal propagation crop, which uses its own cane stems as a provenance for reproduction, and is prone to infection or accumulation of pathogens after many years of continuous planting, which also seriously restricts the annual scale production of sugarcane tissue culture seedlings. In addition, heat treatment combined with shoot apex meristem culture is currently a major technology for cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008A01H4/001
Inventor 刘丽敏何为中刘俊仙刘红坚卢曼曼张荣华李松黄杏
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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