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Rapid propagation method for corylus chinensis by inducing clumped buds

A technology of Chinese hazelnut and induction medium, which can be applied in the biological field and can solve problems such as no Chinese hazelnut

Active Publication Date: 2016-12-07
CHINA THREE GORGES CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are reports on the tissue culture of hazelnuts (combination of flat hazel and hazelnut) in Betaceae Corylus, mainly using the stems of the current year's plants as explants for tissue culture and rapid propagation research. There are no related reports on hazelnut

Method used

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  • Rapid propagation method for corylus chinensis by inducing clumped buds
  • Rapid propagation method for corylus chinensis by inducing clumped buds
  • Rapid propagation method for corylus chinensis by inducing clumped buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Establishment of Stem Rapid Propagation System

[0026] source of explants

[0027] Collect the 7-10d seedlings of the germplasm resource nursery that grow vigorously and are free from diseases and insect pests.

[0028] explant disinfection

[0029] Collect the robust seedlings 7-10 days after sowing and emergence of Huazhen in March, April, and May respectively, remove the roots, keep the cotyledons and remove the upper 2 leaves, wash the dust on the plant surface with tap water, soak in washing powder for 8 minutes, and tap water Rinse for 1 hour, place on an ultra-clean workbench, soak in 75% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride for 15 minutes, wash with sterile water 5-6 times, cut into 2 cm stems with buds, and place them on sterile filter paper to blot dry Surface moisture back up.

[0030] starter medium

[0031] Inoculate the treated material in the starting medium: MS+KT1.0mg·L -1 +IBA0.05mg·L -1 +GA 3 1.0mg·L -1 +Agar 5.8...

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Abstract

The invention relates to a rapid propagation method for corylus chinensis by inducing clumped buds. According to the technical points, the method is mainly characterized in that seedling stem sections and mature embryos of corylus chinensis serve as explants and are planted in initial media, and after the fixed bud induction rate reaches 96.4% or above, the explants are transplanted into a medium for clumped bud induction, wherein one formula of the initial media comprises MS, 1.0 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, the other formula of the initial media comprises MS, 33.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, and the medium for clumped bud induction is prepared from MS, 1.5 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 0.1 g.L<-1> of tryptone, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC; the germination rate is 92%.

Description

technical field [0001] The invention relates to a method for inducing clustered buds to grow rapidly in hazelnut, belonging to the field of biotechnology. Background technique [0002] Huazhen, also known as Shanbaiguo, belongs to the deciduous tree of Betaceae Corylus, which is a rare and precious tree species unique to China. It is a rare large tree in the genus Corylus. Chestnuts have a hard shell, fat white and round kernels, are fragrant, and contain a lot of oil. They are called the "King of Nuts", and they are also called "Four Big Nuts" together with almonds, walnuts, and cashews. [0003] The technology of the present invention adopts the budded stem section of the annual seedlings of the Chinese hazelnut and mature germ embryos as materials, and induces fixed buds and clustered buds by the fixed buds in two ways, so as to establish a rapid propagation regeneration system of the Chinese hazelnut. [0004] At present, there are reports on the tissue culture of hazel...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李翩翩张国禹黄桂云邱利文吴笛吴锦华张海波马晓波胡梅香杨兰芳汪磊
Owner CHINA THREE GORGES CORPORATION
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