Sorbus pohuashanensis stem cells capable of increasing anthocyanin content, culture medium and culture method

Abstract

The invention discloses a sorbus pohuashanensis stem cell capable of increasing anthocyanin content, a culture medium and a culture method, the culture medium can be applied to the culture for synthesizing anthocyanin from the sorbus pohuashanensis stem cell, and the culture method comprises the following specific steps: (1) preparing stem cell tissues; and (2) induction and accumulation of anthocyanin. According to the method, the konjac is used for preparing a natural culture medium, and nutrient substances of the konjac are used for providing macroelements, microelements and a carbon source for stem cells to be cultured; substances contained in the konjak have a promoting effect on stem cell induction, explants expand in 3-5 days, and stem cells are obviously increased in 7-10 days; the konjak culture medium also has the effect of promoting anthocyanin synthesis, and the anthocyanin content is obviously increased; besides, the konjac glucomannan has good coagulability by utilizing the characteristics of the konjac glucomannan, the addition of agar is reduced, the culture cost is saved, and a target product cultured by the natural culture medium is safer and more credible.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture, and in particular relates to a rowan stem cell, a culture medium and a culture method for increasing anthocyanin content. Background technique [0002] Aronia melanocarpa belongs to the family Rosaceae. It is native to eastern North America, and later spread to Europe and the Soviet Union. It was introduced to China for cultivation in the 1990s. The research on the function of polyphenols of Sorbus arvensis began in the 1980s. As its functions in scavenging free radicals, anti-cancer, lowering blood pressure, and softening blood vessels were gradually discovered, relevant The health value of Sorbus polyphenols is also increasingly apparent. Aronia fruit is rich in polyphenols such as anthocyanins, flavonoids, phenolic acids, and proanthocyanidins. Due to the anti-oxidation, free radical scavenging and impact of polyphenols in Aronia fruit Due to the various abilities of human metaboli...

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Problems solved by technology

[0003] At present, the small-scale planting of Sorbus nigra in some places in my country is far from meeting the increasing demand of the market. In addition, in the production process, there are prominent problems such as pests and diseases, production reduction, climate and season restrictions, heavy toxicity and pesticide residues, etc. Moreover, the quality of fruits produced in different regions and in different years is unstable, and the content of active ingredients such as anthocyanins varies greatly, which affects the downstream processing and commercial promotion of Sorbus sagoculus; The high content of chemical components affects the quality and taste of deep-processed products

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