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Method for promoting in-vitro rapid propagation of zingiber mioga

An in vitro technology of succulent lotus, applied in the field of plant tissue culture, can solve the problems of reduced yield, root and stem decline, serious pest infestation, etc., and achieve the effects of strong division ability, vigorous plant growth and short cultivation period

Inactive Publication Date: 2017-07-07
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The subterranean stems are mostly used for reproduction in production, but after 2 to 3 years of continuous propagation, the rhizomes gradually decline, the yield decreases, and the infestation of diseases and insect pests is serious, so it needs to be renewed and propagated.

Method used

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  • Method for promoting in-vitro rapid propagation of zingiber mioga
  • Method for promoting in-vitro rapid propagation of zingiber mioga
  • Method for promoting in-vitro rapid propagation of zingiber mioga

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Effects of BA, TDZ and NAA contents in the proliferation medium on the proliferation of glutinous rice

[0067] The experiment is set up in experimental groups 1-9, and in experimental groups 1-9, the induction medium is: MS medium is used as the basic medium, with 4.0 mg / L BA, 0.1-0.2 mg / L TDZ, 0.9- 1.0mg / L NAA, 25~35g / L sucrose and 6.5~7.5g / L agar medium; the matrix is ​​a mixture of yellow mud, peat and sand with a weight ratio of 3:2:1 In the value-added medium, the content of sucrose is 25-35g / L, and the content of agar is 6.5-7.5g / L.

[0068] In the medium kits used in test groups 1-9, the induction medium and the substrate were the same, and the contents of sucrose and agar in the value-added medium were also the same. The only difference between the medium kits used in experimental groups 1-9 is the different contents of BA, TDZ and NAA in the value-added medium, as follows:

[0069] Group BA content (mg / L) TDZ content (mg / L) NAA content (mg / L) Test group 10.80...

Embodiment 2

[0083] The effect of TDZ content in the proliferation medium on the proliferation of bursa

[0084] The experiment is set up in experimental groups 1 to 2, and in experimental groups 1 to 2, the induction medium is: MS medium is used as the basic medium, plus 4.0 mg / L of BA, 0.1 to 0.2 mg / L of TDZ, 0.9 to 1.0mg / L NAA, 25~35g / L sucrose and 6.5~7.5g / L agar medium; the matrix is ​​a mixture of yellow mud, peat and sand with a weight ratio of 3:2:1 In the value-added medium, the content of sucrose is 25-35g / L, and the content of agar is 6.5-7.5g / L.

[0085] In the medium kits used in test groups 1 to 2, the induction medium and the substrate were the same, and the contents of BA, NAA, sucrose and agar in the value-added medium were also the same. The only difference between the medium kits used in the experimental groups 1-9 is the TDZ content in the value-added medium, which are as follows:

[0086] Group BA content (mg / L) TDZ content (mg / L) NAA content (mg / L) Test group 12.00.0...

Embodiment 3

[0096] An embodiment of the culture medium kit that promotes the rapid propagation of bursa in vitro according to the present invention, the culture medium kit of the embodiment includes an induction medium, a value-added medium, and a substrate;

[0097] The induction medium: MS medium is used as the basic medium, with 4.0 mg / L BA, 0.1 mg / L TDZ, 0.9 mg / L NAA, 25 g / L sucrose, and 6.5 g / L agar Culture medium

[0098] The value-added medium: MS medium is used as the basic medium, with 2.0 mg / L BA, 0.01 mg / L TDZ, 0.04 mg / L NAA, 35 g / L sucrose and 7.5 g / L agar Culture medium

[0099] The matrix: a matrix formed by mixing yellow mud, peat and sand with a weight ratio of 3:2:1.

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Abstract

The invention discloses a culture medium box set for promoting in-vitro rapid propagation of zingiber mioga. The culture medium box set is characterized in that an MS culture medium is used as a basic culture medium, and is added with 1.0 to 3.0mg / L of BA (butyl acrylate), 0.01 to 0.02mg / L of TDZ (thidiazuron), 0.04 to 0.06mg / L of NAA (naphthalene acetic acid), 25 to 35g / L of sugarcane and 6.5 to 7.5g / L of agar, so as to obtain the propagation culture medium. The invention also discloses a method using the culture medium box set to promote the in-vitro rapid propagation of the zingiber mioga. The method has the advantages that under the optimizing conditions, after the zingiber mioga is cultured by the method,; 10 to 15 propagation times can be reached; after 30 days, the rooting rate is 100%; the transplanting survival rate is 100%.

Description

Technical field [0001] The invention relates to a method for culturing plant tissues, in particular to a method for promoting the rapid propagation of Zanthoxylum indicum in vitro. Background technique [0002] Zingiber mioga (Thunberg) Roscoe is a perennial herbaceous plant belonging to the ginger genus of the ginger family. It is also known as Ulva Ulva, Impatiens, Thunberg, and Wild Ginger. There are two types of purple flowers and white flowers. Zhanhe has high edible value and medicinal value. It is mainly eaten from its tender inflorescences, shoots, flower shafts and underground stems. Each 100g of tender stems and flower shafts contains about 12.4g protein, 2.2g fat and 28.1 cellulose. g. Vitamin C and Vitamin A total about 95.85mg, and also contain iron, zinc, selenium and many other trace elements; the rhizomes of Zanhe are warm in nature, pungent in taste, warm the middle and regulate qi, expel wind and relieve pain, reduce swelling, invigorate blood, and dispel silt ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡秀
Owner ZHONGKAI UNIV OF AGRI & ENG
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