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A kind of tissue culture method of Panax notoginseng

A technology of tissue culture and Panax notoginseng, applied in the field of plant tissue culture, to achieve the effects of reducing pollution rate, good callus growth state, and easy operation

Active Publication Date: 2019-06-25
BAISE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the callus culture of Panax notoginseng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Selection and pretreatment of Panax notoginseng explants: select fresh green and healthy Panax notoginseng plants, cut leaves of Panax notoginseng plants as explants, and place them in a beaker; After lightly brushing the surface of the above-mentioned clipped leaves, soak them with 0.1% carbendazim solution for 10 minutes; pour out the carbendazim solution and wrap the mouth of the beaker with gauze, then rinse with running water for 10 minutes;

[0027] (2) Sterilization: Soak the above-mentioned pretreated explants in alcohol with a volume fraction of 75% for 20 seconds, rinse them with sterile water for 3 times, and then use 0.1% of mass fraction and add 2 drops of Tween Soak in -80 mercuric chloride solution for 7 minutes, and finally rinse with sterile water for 5 times;

[0028] (3) Induction culture of explants: inoculate the above-mentioned sterilized explants in the primary culture medium, and carry out dark culture at 26°C for 11-13 days, and a lot of gre...

Embodiment 2

[0032] (1) Selection and pretreatment of Panax notoginseng explants: select fresh green and healthy Panax notoginseng plants, cut the stems of Panax notoginseng plants as explants, and place them in a beaker; After lightly brushing the surface of the above-mentioned clipped leaves in water, soak them in 0.1% carbendazim solution for 17 minutes; pour out the carbendazim solution and wrap the mouth of the beaker with gauze, then rinse with running water for 19 minutes;

[0033] (2) Sterilization: Soak the above-mentioned pretreated explants in alcohol with a volume fraction of 75% for 32 seconds, rinse them with sterile water for 3 times, and then use 0.1% of mass fraction and add 3 drops of Tween Soak in -80 mercuric chloride solution for 9 minutes, and finally rinse with sterile water for 5 times;

[0034] (3) Induction culture of explants: inoculate the above-mentioned sterilized explants in the primary culture medium, and carry out dark culture at 26°C for 11-13 days, and a ...

Embodiment 3

[0038] (1) Selection and pretreatment of Panax notoginseng explants: Select fresh green and healthy Panax notoginseng plants, cut the petioles of Panax notoginseng plants as explants, and place them in a beaker; After lightly brushing the surface of the above-mentioned clipped leaves, soak them in 0.1% carbendazim solution for 20 minutes; pour out the carbendazim solution and wrap the mouth of the beaker with gauze, then rinse with running water for 30 minutes;

[0039] (2) Sterilization: Soak the above-mentioned pretreated explants in alcohol with a volume fraction of 75% for 40 seconds, rinse them with sterile water for 3 times, and then use 0.1% alcohol with a mass fraction of 2-3 drops Soak in mercuric chloride solution of Tween-80 for 10 minutes, and finally rinse with sterile water for 5 times;

[0040](3) Induction culture of explants: inoculate the above-mentioned sterilized explants in the primary culture medium, and carry out dark culture at 26°C for 11-13 days, and ...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly discloses a tissue culture method of panax notoginseng, which includes the following steps: 1) selection and pretreatment of a panax notoginseng explant: cutting leaves as explants and soaking the explants in a carbendazim solution; 2) sterilization: soaking the explants in alcohol and then in a mercuric chloride solution; 3) inducing culture of the explants: inoculating the sterilized explants on a primary culture medium to perform dark culture, thus forming a callus; 4) proliferation culture: inoculating the callus on a proliferation culture medium to perform culture; 5) differentiation culture: inoculating the callus, obtained through the proliferation culture, onto a differentiation culture medium toculture the callus, thus producing differentiated tissue culture seedlings. The tissue culture method is used for explants of panax notoginseng, wherein the callus is high in induction rate and is good in growth status. Meanwhile, the callus has good differentiation effect and can root.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Panax notoginseng. 【Background technique】 [0002] Panax notoginseng is a traditional and precious Chinese medicinal material in my country, which is a perennial erect herb of Araliaceae. The whole body of Panax notoginseng is a treasure, and its stems, leaves, flowers, and fruits are also used as medicine in the folk, which has high medicinal value, but the roots are mainly used as medicine. The dried roots are warm in nature, dry and bitter, and sweet in taste. 24 kinds of notoginseng saponins, 17 kinds of amino acids, notoginseng polysaccharides, notoginseng flavonoids and other physiologically active substances have various external effects such as dispelling blood stasis, reducing swelling and relieving pain, and have the effect of lowering blood sugar and blood fat. [0003] However, at present, the wild resources of Panax noto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 农艳丰李健陶柳臻谢秀凤
Owner BAISE UNIV
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