Rapid Breeding Method of Leopard
A leopard and big-flowered technology, applied in the field of rapid reproduction of big-flowered leopards, can solve the problems that have not yet been found in artificial cultivation and breeding research reports of big-flowered leopards, so as to facilitate large-scale industrial production and application, reduce the possibility of variation, and take root The effect of high survival rate of transplanting
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Embodiment 1
[0027] A rapid propagation method of Leopard grandiflora, using mature berries of Leopard grandiflora as explants, after disinfection and sterilization, the berries are cut open under aseptic conditions to take out the seeds of Leopard grandiflora, and the germinated seedlings obtained through germination and cultivation are successively carried out. Primary culture, subculture, rooting culture and seedling transplanting.
[0028] Wherein, the Leopard grandiflora seeds are inoculated onto the germination medium and germinated and cultivated for 7-10 days to obtain germinated seedlings, the formula of the germination medium is: MS+1.5mg / L 6-BA+0.5mg / LGA 3 +300mg / L PVP, the germination culture conditions are: temperature 24-28 ℃, light intensity 2000Lx, light time 10h / day.
[0029] Among them, the method of disinfection and sterilization of mature berries of Leopard grandiflora as explants is as follows: take mature berries of Leopard grandiflora, soak them in alcohol with a vol...
Embodiment 2
[0036] A method for rapid propagation of leopards with large flowers. On the basis of embodiment 1, the explants are directly inoculated into the induction medium for primary culture with tender shoots or stem segments with buds. Others are the same as in Example 1.
[0037] The method of the present embodiment 2 cannot induce clustered buds.
Embodiment 3
[0039] A method for rapid propagation of leopards. On the basis of Example 1, the formula of the proliferation medium is replaced by: MS, 6-BA 1.5mg / L, KT 0.75mg / L, NAA 1.0mg / L.
[0040] Except the formula of proliferation medium, others are identical with embodiment 1.
[0041] The method of Example 3 can realize the multiplication of clustered buds, the multiplication factor is 3.46 times, and the transplanting survival rate reaches 90.3%.
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