Rapid Breeding Method of Leopard

A leopard and big-flowered technology, applied in the field of rapid reproduction of big-flowered leopards, can solve the problems that have not yet been found in artificial cultivation and breeding research reports of big-flowered leopards, so as to facilitate large-scale industrial production and application, reduce the possibility of variation, and take root The effect of high survival rate of transplanting

Inactive Publication Date: 2018-06-05
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after reviewing the literature, there is no research report on the artificial cultivation and breeding of leopards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A rapid propagation method of Leopard grandiflora, using mature berries of Leopard grandiflora as explants, after disinfection and sterilization, the berries are cut open under aseptic conditions to take out the seeds of Leopard grandiflora, and the germinated seedlings obtained through germination and cultivation are successively carried out. Primary culture, subculture, rooting culture and seedling transplanting.

[0028] Wherein, the Leopard grandiflora seeds are inoculated onto the germination medium and germinated and cultivated for 7-10 days to obtain germinated seedlings, the formula of the germination medium is: MS+1.5mg / L 6-BA+0.5mg / LGA 3 +300mg / L PVP, the germination culture conditions are: temperature 24-28 ℃, light intensity 2000Lx, light time 10h / day.

[0029] Among them, the method of disinfection and sterilization of mature berries of Leopard grandiflora as explants is as follows: take mature berries of Leopard grandiflora, soak them in alcohol with a vol...

Embodiment 2

[0036] A method for rapid propagation of leopards with large flowers. On the basis of embodiment 1, the explants are directly inoculated into the induction medium for primary culture with tender shoots or stem segments with buds. Others are the same as in Example 1.

[0037] The method of the present embodiment 2 cannot induce clustered buds.

Embodiment 3

[0039] A method for rapid propagation of leopards. On the basis of Example 1, the formula of the proliferation medium is replaced by: MS, 6-BA 1.5mg / L, KT 0.75mg / L, NAA 1.0mg / L.

[0040] Except the formula of proliferation medium, others are identical with embodiment 1.

[0041] The method of Example 3 can realize the multiplication of clustered buds, the multiplication factor is 3.46 times, and the transplanting survival rate reaches 90.3%.

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PUM

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Abstract

The invention discloses a rapid propagation method of campanumoea javanica. The method comprises the following steps: ripe berries of campanumoea javanica are used as explants and undergo disinfection and sterilization; under aseptic condition, berries are cut open and seeds of campanumoea javanica are then taken out; through germination and cultivation, germinating seedlings are obtained; and finally, the germinating seedlings successively undergo primary culture, subculture, rooting culture and acclimatization and transplanting. By using ripe berries of campanumoea javanica as explants, germinating and culturing the seeds and inducing cluster buds without the callus phase. Therefore, the possibility of variation is greatly reduced, and the method is beneficial to keep excellent traits of seedlings.

Description

technical field [0001] The invention relates to the technical field of tissue culture of leopards. More specifically, the present invention relates to a method for rapid breeding of the big-flowered leopard. Background technique [0002] The big-flowered leopard is Campanumoea javanica, a plant of the Campanulaceae family, also known as platycodon grandiflorum, grandma root, Chai Codonopsis (Guizhou), Gui Codonopsis (Guangdong), four-edged ginseng, and oxtail ginseng (Yunnan). Herbaceous twining vines, latexy, with carrot-like roots. Stems glabrous, much branched. Leaves opposite, rarely alternate, long stalked, heart-shaped or heart-shaped-ovate, margins shallowly serrated, rarely entire, 3-11 cm long, 2-9 cm wide, glabrous or sometimes abaxially Thin hairs. Flowers single in leaf axils, each part glabrous, calyx separated from ovary, 5-lobed to near base, lobes ovate-lanceolate or lanceolate, 1-1.8 cm long; corolla large, corolla superior, white or yellow-green, inner ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/00A01N65/08A01N59/00A01P21/00
CPCA01G9/0299A01H4/008A01N59/00A01N65/00A01N65/08A01N2300/00
Inventor 姚绍嫦蓝祖栽凌征柱
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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