Food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array

Abstract

The invention discloses a food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array. The food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array comprises a substrate, and a pathogenic bacterium, a positive control and a negative control are fixed on the substrate. The pathogenic bacterium is one or more of staphylococcus aureus, hemorrhagic escherichia coli O157: H7, Listeria monocytogenes, salmonella cholerae suis, salmonella typhimurium, salmonella enteritidis, Shigella flexneri, Shigella sonnei, shigella dysenteriae, Shigella boydii, vibrio parahaemolyticus, enterobacter sakazakii, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi B. According to the food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array, multi-target screening can be carried out, dozens of hybridoma cells can be screened within 2.5 hours, the preparing period of food-borne pathogenic bacterium monoclonal antibody is greatly shortened, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an antigen macroarray, in particular to an antigen macroarray for rapid screening of food-borne pathogenic bacteria hybridoma cells. Background technique [0002] Food safety is a major public security issue related to the national economy and the people's livelihood, and it is also one of the key points of the supervision work of the national administrative agency. In recent years, domestic food safety accidents have emerged one after another, and food-borne pathogenic bacteria contamination is the most common problem in food safety. According to the report of the World Health Organization (WHO), the number of cases of diarrheal diseases in the world is as high as 150 million every year, and 70% of the cases are related to food contaminated by various pathogenic microorganisms. The research data of my country's CDC also shows that microorganisms are the main pathogens of foodborne ...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects existing in the above-mentioned existing food-borne pathogen hybridoma cell screening technology, the present invention provides a food-borne pathogen hybridoma rapid screening antigen macroarray. Antigen macroarray for rapid screening of pathogen hybridoma cells needs to solve the technical problems of high cost, time-consuming, complicated and cumbersome screening technology of food-borne pathogen hybridoma cells

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