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Activated probiotic skin cream and preparation method thereof

A technology of active probiotics and skin cream, which is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of easy decline and inactivation of probiotics, unapplied probiotics, and low stability of live bacteria. Achieving the effect of enriching varieties, efficient biological protection, and balancing the pH of the skin surface

Active Publication Date: 2013-08-21
广州集妍化妆品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the application of active probiotics in skin care products is mainly limited by two factors. On the one hand, probiotics on the market are mainly in the form of powder or granules, and cannot withstand process temperatures higher than 45°C, so they cannot be directly added to skin care products In the emulsified system, it cannot be integrated into a uniform product; on the other hand, probiotics are easily degraded and inactivated in a system without any protective agent and exposed to a large amount of free water, and the stability of live bacteria is low
Therefore, active probiotics have not been used in daily skin care products, and there are no related reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In the first step, the Streptococcus thermophilus (Streptococcsus thermophilus) is cultured at a high density at 40°C in the medium in the microbial batch culture fermenter, and the number of viable bacteria in the culture solution reaches 6.5×10 9 CFU / mL, and then centrifuged at 15000rpm for 5 minutes to obtain the sludge, the number of viable bacteria in the sludge was 1×10 11 CFU / g;

[0028] The second step is to mix glycerin and trehalose according to the mass ratio of 80:20, and after the trehalose is completely dissolved in a 90°C water bath, heat to 95°C and keep it for 15 minutes to sterilize, then seal and cool to 4°C;

[0029] In the third step, mix the bacterium slime obtained in the first step with the mixed solution obtained in the second step at a mass ratio of 1:10, grind and stir evenly, and become a bacterium slurry;

[0030] The fourth step is to filter the bacterial slurry with a 200-mesh sieve, and the filtrate is a probiotic bacterial liquid, and t...

Embodiment 2

[0034] In the first step, Lactobacillus casei subsp casei is cultured at a high density at 37°C in the medium in the fermentation tank for batch culture of microorganisms, and the number of live bacteria in the culture solution reaches 1.2×10 9 CFU / g, and then centrifuged at 10000rpm for 10 minutes to obtain the sludge, the number of viable bacteria in the sludge was 3×10 10 CFU / g;

[0035] The second step is to mix glycerin and trehalose according to the mass ratio of 85:15. After the trehalose is completely dissolved in an 85°C water bath, heat at 90°C and keep it for 20 minutes. After sterilization, seal and cool, and refrigerate at 4°C;

[0036] In the third step, the bacterium slime obtained in the first step is mixed with the mixed solution obtained in the second step at a mass ratio of 1:100, and ground and stirred evenly to become a bacterium slurry;

[0037] The fourth step is to filter the bacterial slurry with a 300-mesh sieve, and the filtrate is a probiotic bacte...

Embodiment 3

[0041] In the first step, the Streptococcus thermophilus (Streptococcsus thermophilus) is cultured at a high density at 40°C in the medium in the microbial batch culture fermenter, and the number of viable bacteria in the culture solution reaches 6.5×10 9 CFU / g, and then centrifuged at 15000rpm for 5 minutes to obtain the sludge, the number of live bacteria in the sludge was 1×10 11 CFU / g;

[0042] In the second step, Lactobacillus casei subsp casei is cultured at a high density at 37°C in the medium in the fermentation tank for batch culture of microorganisms, and the number of live bacteria in the culture solution reaches 1.5×10 10 CFU / g, and then centrifuged at 15000rpm for 10 minutes to obtain the sludge, the number of viable bacteria in the sludge was 6×10 11 CFU / g;

[0043] The third step is to mix glycerin and trehalose according to the mass ratio of glycerin and trehalose at 90:10. After the trehalose is completely dissolved in a 90°C water bath, heat at 95°C and kee...

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Abstract

The invention discloses activated probiotic skin cream and a preparation method thereof. The method comprises the steps of carrying out high-intensity cultivation on probiotic, thereby obtaining bacterial sludge from culture solution by centrifugation; mixing glycerol with mycose; heating in a water bath until the mycose is completely dissolved; tightly cooling to 0-4 DEG C after sterilizing; mixing the obtained bacterial sludge with a mixing solution of glycerol and mycose, grinding and stirring evenly to form bacterial vaccine, filtering to obtain activated probiotic bacteria liquid; preparing a water-in-oil emulsion system, adding the activated probiotic bacteria liquid and a daily essence to the water-in-oil emulsion system, and evenly stirring to obtain the activated probiotic skin cream, wherein the obtained activated probiotic skin cream contains 1*10<6> to 1*10<8> CFU / g of activated probiotic. The skin cream disclosed by the invention can ensure the activity and the quality stability of the probiotic in the production and product storage processes; and effects of cleaning the skin by the probiotic, balancing the skin surface pH, restraining harmful bacteria, preventing damage of heavy metal ions on the cell, holding water and moisturizing are finally achieved.

Description

technical field [0001] The invention belongs to the technical field of probiotic application, and in particular relates to an active probiotic skin cream and a preparation method thereof. Background technique [0002] The development trend of today's cosmetics is the trend of biologicalization, functionalization and application of high technology. Probiotics refer to bacteria that are beneficial to human and animal organisms. After more than a century of experiments, it has been proved that probiotics have important physiological functions, and are currently mostly used in health food. There are 10 probiotic strains allowed to be used in health food in my country, including Lactobacillus acidophilus, Lactobacillus casei subsp casei, and Streptococcus thermophilus. [0003] Experiments have proved that applying probiotics directly or blended into skin care products on the facial skin can play a role in skin care and cleansing. Probiotics can absorb and utilize skin excreti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/06A61Q19/00
Inventor 熊犍鲍志宁林伟锋陈中
Owner 广州集妍化妆品科技有限公司
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