7 results about "TATA box" patented technology

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a design method of a stimuli-responsive high-starting-activity heat shock protein promoter. Comprising four HSP promoter design rules: (1) an HSP promoter sequence comprises four elements of an HSE, a TATA box, a GC box and a CCAAT box; (2) in an HSP promoter sequence, repeating three elements, namely an HSE box, a GC box and a CCAAT box for a plurality of times; (3) in the HSEs of the HSP promoter sequence, at least one HSE is a tetramer or multimer HSE; and (4) taking at least one GC box or at least one CCAAT box or at least one GC box and at least one CCAAT box which are close to each other as a whole, and spacing at least one HSE. The heat shock protein promoter designed by the method has the characteristics of multi-species universality, high starting activity after stimulation and capability of artificially adjusting the activity.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention is in the field of polynucleotides. The invention relates to a method for transcription and / or expression using a nucleic acid construct that is characterized by the presence of a TATA box promoter that has been modified to disable the TATA box. This mutated promoter is followed by a second promoter. The invention further relates to that nucleic acid construct, to an expression vector or a cell comprising that construct, and to its use for improved transcription.

The present disclosure provides high-performance immune-activation reporter cell line set. A set of nucleic acid constructs comprising one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter are provided. A set of reporter cell lines comprising a polynucleotide sequence encoding a reporter protein under the control of one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter is also provided. The provided set of reporter cells enable sensitive and signal-enhanced bioassay performance to reflect and evaluate adaptive immune cell activation / inhibition regulated by the immune receptors CD3, co-stimulatory CD28 and related signaling pathways, and avoid responding bias triggered by particular transcription factor. A controllable engineer method for a functional cell line is further provided.

The invention is in the field of polynucleotides. The invention relates to a method for transcription using a nucleic acid construct that is characterized by the presence of a promoter that has been modified to disable the TATA box. This mutated promoter is followed by a second promoter. The invention further relates to that nucleic acid construct, to an expression vector or a cell comprising that construct, and to its use for improved transcription. Production of long RNA sequences was found to be improved by this construct.

The invention provides, e.g., a recombinant virus comprising (i) a modified TATA box-based promoter, and / or (ii) a modified CAAT box-based promoter operably linked to a gene, wherein the modified TATA box-based promoter and / or modified CAAT box-based promoter lacks a functional TATA box and / or CAAT box and permit selective expression of the gene in a hyperproliferative cell. The recombinant viruses can be used to treat cell proliferative diseases and disorders, including certain forms of cancer.

The present invention relates to small secreted reporter-peptides 15 to 150 amino-acids in length comprising a response element, activated by one or more transcription factors induced by the pharmacology active substance to be analyzed following its interaction with a specific intracellular or cell surface molecule, functionally linked to a response element, a TATA box, a signal peptide, anchor and detection sequences to which antibodies can be raised, and a poy-A tail. The anchor sequence may differ from one peptide to another or may be common to multiple secreted reporter-peptides such that all the peptides can be analyzed simultaneously by ELISA using a detection antibody labelled with for example HRP, or by the use of a commonly available immuno-detection platform such MSD, Gyros, AlphaLisa, or Biacore. The detection sequence is unique to each secreted reporter-peptide and may be labelled with a Sulfo-Tag that permits detection of the peptide on the MSD platform, or Alexa that permits detection on the Gyros platform, or digoxigenin that permits detection of on the PerkinElmer AlphaLISA platform, or left unlabeled for detection by SPR on a Biacore platform. The present invention provides i.a. a substantial improvement of cell-based assays for analysis using automated immune-detection platforms and allows simultaneous analysis of multiple analytes, multiple sampling from a single cell culture, and obviates the necessity to lyse cells and remove cell debris by centrifugation prior to analysis on an automated immuno-detection platform. The present invention also provides a means of increasing the dynamic range, sensitivity and reducing the cost of cell-based assays and can be applied to existing engineered cell lines, such as those containing a reporter-gene such as a luciferase reporter-gene obviating the necessity to extensively re-engineer cell lines containing multiple molecular constructs.