4 results about "TATA box" patented technology

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention is in the field of polynucleotides. The invention relates to a method for transcription and / or expression using a nucleic acid construct that is characterized by the presence of a TATA box promoter that has been modified to disable the TATA box. This mutated promoter is followed by a second promoter. The invention further relates to that nucleic acid construct, to an expression vector or a cell comprising that construct, and to its use for improved transcription.

The present disclosure provides high-performance immune-activation reporter cell line set. A set of nucleic acid constructs comprising one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter are provided. A set of reporter cell lines comprising a polynucleotide sequence encoding a reporter protein under the control of one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter is also provided. The provided set of reporter cells enable sensitive and signal-enhanced bioassay performance to reflect and evaluate adaptive immune cell activation / inhibition regulated by the immune receptors CD3, co-stimulatory CD28 and related signaling pathways, and avoid responding bias triggered by particular transcription factor. A controllable engineer method for a functional cell line is further provided.

The invention is in the field of polynucleotides. The invention relates to a method for transcription using a nucleic acid construct that is characterized by the presence of a promoter that has been modified to disable the TATA box. This mutated promoter is followed by a second promoter. The invention further relates to that nucleic acid construct, to an expression vector or a cell comprising that construct, and to its use for improved transcription. Production of long RNA sequences was found to be improved by this construct.