Inducible shRNA (short hairpin ribonucleic acid) lentiviral expression vector and construction method and application thereof

A technology of expression vector and lentivirus, which is applied in the field of functional genomics research and can solve problems such as difficult operation
CN103255172AInactive Publication Date: 2013-08-21傅开屏 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
傅开屏
Publication Date
2013-08-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an inducible shRNA (short hairpin ribonucleic acid) lentiviral expression vector and a construction method and application thereof. The inducible shRNA lentiviral expression vector comprises an shRNA expression frame and a tetracycline repressor protein expression frame, wherein the shRNA expression frame comprises a first promoter, a tetracycline response element being combined with tetracycline repressor protein, a TATA box and an shRNA coding sequence, and the tetracycline repressor protein expression frame comprises a second promoter, a tetracycline repressor protein expression gene, an internal ribosome entry site (IRES) DNA (deoxyribonucleic acid) sequence and a marker gene. Compared with an existing inducible shRNA expression vector, the inducible shRNA lentiviral expression vector has the advantages of being capable of rapidly establishing inducible shRNA expressing a silent gene in various cell strains by using a single vector, having no need of clone selection of cells, and being applicable to in vitro culture and in vivo study of cells.
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Description

technical field

[0001] The invention belongs to the field of functional genomics research, and relates to a lentiviral expression vector, in particular to an inducible shRNA lentiviral expression vector and its construction method and application. Background technique

[0002] RNA interference (RNA interference, RNAi) refers to the phenomenon of efficient and specific degradation of homologous mRNA induced by double-stranded RNA (double-stranded RNA, dsRNA), which is highly conserved during evolution.

[0003] In recent years, RNAi research has made breakthroughs. Because RNAi technology can specifically knock out or shut down the expression of target genes, this technology has been widely used in the field of gene therapy for exploring gene functions and infectious diseases and malignant tumors.

[0004] In order to successfully implement RNAi, effective siRNA must first be obtained. The core of RNAi requires siRNA to effectively bind and act on the corresponding mRNA. T...

Claims

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