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Cotton tissue specific and pathogenic bacterium inducing promoter and its use

A tissue-specific, promoter technology, applied in the biological field, can solve the problems of cotton disease resistance gene isolation and functional research that have not been reported.

Inactive Publication Date: 2007-01-24
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

So far, the isolation and functional studies of the promoters of cotton disease resistance genes have not been reported

Method used

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  • Cotton tissue specific and pathogenic bacterium inducing promoter and its use
  • Cotton tissue specific and pathogenic bacterium inducing promoter and its use
  • Cotton tissue specific and pathogenic bacterium inducing promoter and its use

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Embodiment Construction

[0025] The methods used in the following embodiments are conventional methods unless otherwise specified. The primer sequences used are all synthesized by Shanghai Boya Biotechnology Co., Ltd., and the percentages are all percentages by mass. The upland cotton (Gossypium.hirsutum) varieties used in this experiment are all Chang Kang cotton (Lu Haosheng, Pu Weixin, Huangfu Guohua, Yin Huiyu, Dai Xin. A new high-resistance to deciduous verticillium wilt-Chang Kang cotton. China Cotton 1997 11:26-27.), Arabidopsis varieties are Colombian (Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, BowmanCL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, AdamsMD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC. Sequence and analysis of chromosome 2 of the plant Arabidopsis ...

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Abstract

The present invention relates to cotton tissue specific and pathogenic bacterium inducing promoter and their application, and belongs to the field of biotechnology. The promoter contains CAAT-box, TATA-box, ethylene, methyl jasmonate, abscisic acid response element, pathogenic bacterium inducer response elements W boxes, GT-1, MYB, MYBST1, MYB1LEPR, etc. There are also specific root expressing elements to enhance the expression of GUS gene, which expresses specifically in the phloem of root, bud and stem, the vein of foliage and the guard cell of air pore. Paraffin section observation shows that GHNBS promoter expresses in phloem companion cell. After treatment with SA, MeJA, Ethylene, pathogenic bacteria of blight and pseudomonas syringae DC3000, GUS will have obviously raised activity, so that it is one organ specific and pathogenic bacterium relevant promoter.

Description

1. Technical Field [0001] The invention relates to a plant gene promoter and its application, and provides a cotton tissue-specific and pathogen-inducible promoter. It belongs to the field of biotechnology. Used to improve plant disease resistance and other beneficial production traits through plant genetic engineering technology. 2. Background technology [0002] Plant diseases caused by fungi, bacteria, viruses, and nematodes have a huge impact on crop production. Plants have two different sets of resistance mechanisms: one is the passive defense mechanism, which refers to some resistance-related morphological structures, such as the epidermis and the hard cell wall (Brady JD, Fry S. Formation of di-isodityrosine and loss of isodityrosine in the cell walls of tomato cell-suspension cultures treated with fungal elicitors or H2O2. PlantPhysiol 1997, 115: 87-92.). The other is active defense, which is caused by the identification and interaction of resistance genes with pathogens....

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82
Inventor 张保龙倪万潮杨郁文沈新莲佘建明何晓兰张香桂徐英俊姚姝
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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