A method for batch capture of promoters

Abstract

The invention provides a method for capturing promoters in batches. The method uses the complex of "DNA-protein factors" formed in the promoter region when plant genes are transcribed, and applies the improved chromosomal immunoprecipitation technique to capture the unknown plant DNA sorting out of gene promoter fragments. The present invention utilizes the property that the protein factors OsTBP2 and OsTFIIB tightly bind to the TATA box short-chain conserved sequence on the promoter to initiate transcription, and generates specific antibodies for these two factors, combined with chromatin immunoprecipitation (ChIP), to specifically target A technique for capturing promoter fragments of unknown plant genes. This technology utilizes the characteristic of a short-chain conserved sequence of the TATA box contained in most promoters, combined with the newly developed chromatin immunoprecipitation technique (ChIP) in recent years to isolate unknown plant gene promoter fragments, it is relatively easy Achieving large-scale capture of plant promoters has high commercial value.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a method capable of capturing promoters from plants in batches. This method utilizes the "DNA-protein factor" complex formed in the promoter region when plant genes are transcribed, through improved chromosomal immunity Co-precipitation technique, using antibodies specific for protein factors in the complex, to isolate unknown gene promoter fragments from plant genomes. Background technique [0002] In the process of plant growth and development, the regulatory sequence of the gene, namely the promoter, plays a decisive role in the formation and growth of the specific tissue structure of the plant in different growth stages, as well as the stress response to external biotic and abiotic factors. The conserved sequence in the promoter region is the target of transcription factors, and the specific combination of th...

AI Technical Summary

Problems solved by technology

However, compared with other organisms, the gene discovery of plants is still quite limited in terms of quantity and quality, which is far from meeting the needs of molecular breeding of crops.

In particular, the research and discovery of promoters are mostly based on the analysis, understanding, isolation and expression of individual genes, making it difficult to achieve large-scale and systematic development

[0005] There are also some methods for finding promoters in plant genes in the prior art. However, the methods in the prior art often estimate the possible existence of promoters through software calculations, and cannot directly obtain a large number of promoters at one time.

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