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46 results about "Dna protein" patented technology
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DNA is NOT a protein. DNA is made up from a chain of nucleotides. Proteins are built from a long chain of amino acids. Apart from the fact they're chaining together separate smaller molecules, they have very little in common. 3 nucleotides in DNA translate to one specific amino acid and there is some duplication.
Surface plasmon resonance imaging of micro-arrays
InactiveUS20030017579A1Material nanotechnologyBioreactor/fermenter combinationsSpr imagingMicrofluidic channel
Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and / or germanium surfaces; a muL detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.
Owner:WISCONSIN ALUMNI RES FOUND
DNA-protein fusions and uses thereof
InactiveUS7195880B2Easy to handleGood chemical stabilityBioreactor/fermenter combinationsPeptide librariesProtein neddylationDna protein
Owner:BRISTOL MYERS SQUIBB CO
Method of identifying active chromatin domains
The invention provides a method of mapping DNA-protein interactions within a genome by fixing living cells to cross-link DNA and proteins, lysing the cells, and isolating chromatin by immunoprecipitation. DNA is purified and a SAGE protocol is performed on the purified DNA to produce GMAT-tag sequences, which are compared to a genomic sequence of the living cells to map DNA-protein interactions. The invention further provides a method of identifying an active chromatin domain and a method of identifying aberrant chromatin acetylation, wherein chromatin immunoprecipitation is performed using an antibody recognizing acetylated histone protein.
Owner:GOVERNMENT OF THE US REPRESENTED BY THE SEC
Plasmon-enhanced marking of fragile materials and other applications thereof
InactiveUS20050142605A1Resonance-enhanced multiband absorptionPromote absorptionBioreactor/fermenter combinationsMaterial nanotechnologyFluorescencePaper document
The methods and applications of a surface plasmon resonance-enhanced marking technique are disclosed. The technique uses surface plasmon resonance (SPR) excited nanoparticles and a surface plasmon resonance source in nonlinear interactions with nearby chemical substances and medium for marking purposes. The SPR-enhanced absorption and fluorescence rates of chemical substances or medium and nonlinearity of SPR interactions with chemical substances or medium make the proposed method suitable for marking fragile materials including biomaterials, such as writing on thin plastic foils or DNA-protein crosslinking. The marking method can also be applied to a three-dimensional recording and read out information system with subwavelength resolutions, coding information of secrete documents, drug delivery, tissue surgery, tattoo writing or removal, photodynamic therapy, cosmetic and dermatological treatment.
Owner:SPR ADVANCED TECH INC
Surface plasmon resonance compatible carbon thin films
ActiveUS20090141376A1High refractive indexPrismsParticle separator tubesHigh-Density MicroarrayPhotolithography
SPR-compatible substrates for high density microarray fabrication and analyses are provided. Novel carbon-on-metal thin film substrate architecture permits the integration of surface plasmon resonance detection with photolithographically fabricated biomolecule arrays for the analysis of biomolecular interactions. The utility of the technology is shown in the analysis of specific DNA-DNA, DNA-RNA and DNA-protein binding interactions. These new substrates may be used to determine the secondary structure of RNA molecules, to probe the sequence-specific binding kinetics and affinity of proteins and small molecules, and as substrates for small-molecule combinatorial chemistry platforms for drug discovery applications.
Owner:WISCONSIN ALUMNI RES FOUND
Plasmon-enhanced marking of fragile materials and other applications thereof
InactiveUS7462496B2Resonance-enhanced multiband absorptionPromote absorptionMaterial nanotechnologyBioreactor/fermenter combinationsFluorescenceWavelength
The methods and applications of a surface plasmon resonance-enhanced marking technique are disclosed. The technique uses surface plasmon resonance (SPR) excited nanoparticles and a surface plasmon resonance source in nonlinear interactions with nearby chemical substances and medium for marking purposes. The SPR-enhanced absorption and fluorescence rates of chemical substances or medium and nonlinearity of SPR interactions with chemical substances or medium make the proposed method suitable for marking fragile materials including biomaterials, such as writing on thin plastic foils or DNA-protein crosslinking. The marking method can also be applied to a three-dimensional recording and read out information system with subwavelength resolutions, coding information of secrete documents, drug delivery, tissue surgery, tattoo writing or removal, photodynamic therapy, cosmetic and dermatological treatment.
Owner:SPR ADVANCED TECH INC
Therapeutic nucleic acid-3' -conjugates
InactiveUS20050191680A1Efficient killingPrevent breakdownMaterial nanotechnologyActivity regulationHalf-lifeWhite blood cell
Methods are described for improvement of the serum half life of therapeutic nucleic acids by 3′ conjugation to useful target proteins, or other large molecules with useful function. In one embodiment, a 3′ A, C or G overhang is added to ds-DNA and the primary amines conjugated using biocompatible bifunctional linkers to proteins. The resulting nucleic acid-3′-conjugates are serum nuclease-resistant and retained in vivo for long periods without rapid kidney clearance. Further, the choice of conjugate imparts additional functionality to the nucleic acid-3-conjugate. For example, if the protein in the DNA-protein conjugate is the first component of the complement cascade (Clq or Clqrs) and the DNA aptamer has been developed against surface components of a target cell, it can be used to treat bacterial or parasitic infections and cancers. If the protein is serum albumin or another common (nonimmunogenic) blood protein and the aptamer is directed against a toxin or venom, the aptamer-protein conjugate can be used as an antidote that binds and neutralizes the toxin or venom. Similar DNA (aptamer)-nanotube, -enzyme, and -toxin conjugates could also be used to target and selectively kill bacteria, parasites, and cancer cells in vivo. If the protein is an Fc antibody fragment or C3b protein from the complement system and the aptamer is developed against a bacterial cell capsular material, other cell surface component or viral cell surface component, then the aptamer-3′-protein conjugate can aid in opsonization of the target cells or viruses by phagocytic leukocytes.
Owner:OTC BIOTECH
Ensemble learning method for predicting DNA protein binding site
PendingCN108763865AGood effectReduce imbalanceNeural architecturesSpecial data processing applicationsBinding siteA-DNA
The invention relates to an ensemble learning method for predicting a DNA protein binding site. The method comprises the following steps of obtaining protein sequence data of the DNA protein binding site; preprocessing the protein sequence data of the DNA protein binding site; establishing input data in a one-hot coding mode; combining extracted features, establishing features of amino acid on each protein sequence, and taking the features as the input data; carrying out oversampling on positive sample data through utilization of an SMOTE algorithm; dividing negative sample data into a plurality of parts according to the positive sample quantity, and combining each part of negative samples with the positive samples to form a new data subset, thereby obtaining N data subsets; training eachdata subset through utilization of convolutional neural networks; and integrating results of N convolutional neural networks through utilization of a majority voting method, thereby obtaining a predicting result. According to the method, the DNA protein binding site predicting problem under the imbalance data condition is solved, and the predicting accuracy is improved.
Owner:CHENGDU UNIV OF INFORMATION TECH
Nickel-based reagents for detecting DNA and DNA-protein contacts
The invention relates to a compound comprising a labeled nickel complex (FIG. 1) that is used to detect and purify nucleic acid sequences by forming an adduct between a specific nucleic acid base and the labeled nickel complex. The adduct can also be purified through a separation process, such as affinity chromatography
Owner:THE UNIV OF UTAH +1
Bidirectional LSTM and CNN model for predicting DNA-protein binding
InactiveCN109559781AEasy to learnImprove predictive performanceSequence analysisNeural architecturesAlgorithmComplete sequence
The invention provides a bidirectional LSTM and CNN model for predicting DNA-protein binding. The model includes an input layer, a BLSTM layer, a convolutional layer, a maximum pooling layer, a full connection layer, and an output layer. Each input sequence is expressed as a four-line binary matrix by the input layer through single thermal coding. In the BLSTM layer, each LSTM model in a previouslayer will receive information of interest on DNA from an input sequence, and encode and interpret contributions from past historical information to a hidden state; then, the BLSTM module is propagated to the next BLSTM module, wherein a matrix scanned and input by each convolution kernel in the convolution layer is used for motif discovery, and information with different intensities is associatedwith potential sequence patterns; the maximum pooling layer is used for maximizing an output signal of each convolution kernel to form a complete sequence; the output layer performs non-linear conversion to determine DNA-protein binding feature information.
Owner:CHENGDU UNIV OF INFORMATION TECH
Double stranded nucleic acid biochips
InactiveUS7049073B2Convenience to mergeSugar derivativesNucleotide librariesDouble strandNon enzymatic
This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA / dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.
Owner:CHICAGO UNIV OF +1
Surface plasmon resonance imaging of micro-arrays
InactiveUS7670556B2Small amountSave resourcesBioreactor/fermenter combinationsMaterial nanotechnologySpr imagingMicrofluidic channel
Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and / or germanium surfaces; a μL detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.
Owner:WISCONSIN ALUMNI RES FOUND
Method of identifying active chromatin domains
The invention provides a method of mapping DNA-protein interactions within a genome by fixing living cells to cross-link DNA and proteins, lysing the cells, and isolating chromatin by immunoprecipitation. DNA is purified and a SAGE protocol is performed on the purified DNA to produce GMAT-tag sequences, which are compared to a genomic sequence of the living cells to map DNA-protein interactions. The invention further provides a method of identifying an active chromatin domain and a method of identifying aberrant chromatin acetylation, wherein chromatin immunoprecipitation is performed using an antibody recognizing acetylated histone protein.
Owner:GOVERNMENT OF THE US REPRESENTED BY THE SEC
Method for extracting DNA from old formalin-fixed tissues
InactiveCN101787364AQuality improvementHigh puritySugar derivativesSugar derivatives preparationLarge fragmentA-DNA
The invention discloses a method for extracting DNA from old formalin-fixed tissues. The basic principle of the method is to utilize the microwave thermal effect for fast removing formalin in the fixed tissues via water molecules, and eliminate the DNA-protein crosslinking caused by aldehyde groups of the formalin, thereby completely or partially recovering a DNA structure and further obtaining the DNA with better quality. The invention relates to the simple and high-efficient method for extracting the DNA from the old formalin-fixed tissues, which is based on microwave thermal remediation and can obtain the DNA with higher quality (large fragments and high purity), so that the method can be used for multiplex fluorescent PCR-capillary electrophoresis (FM-CE), denaturing high performance liquid chromatography (DHPLC) analysis, direct DNA sequencing and other molecular biological method analyses.
Owner:ZHEJIANG UNIV
DNA-protein conjugate and application thereof in detecting mercury ion concentration
The invention belongs to the technical field of detection and analysis, and particularly discloses a DNA-protein conjugate and application thereof in detecting mercury ion concentration. By sufficiently utilizing the FRET effect between a DNA chain and a nucleic acid chain complementary with the DNA chain and the affinity reaction between the DNA-protein conjugate and protein ligand fixed to an evanescent wave sensing chip, a method capable of effectively detecting the mercury ion concentration is obtained. Compared with an existing method, the method has the advantages of being economical, and easy, convenient and rapid to implement and has the important social and economic significance.
Owner:TSINGHUA UNIV
Methods, compositions and kits for increasing genome editing efficiency
Methods of editing a target genomic region(s), methods of repairing of a DNA break via a HDR pathway, methods of inhibiting or suppressing repair of a DNA break via a NHEJ pathway, and methods of modifying expression of a gene(s) or protein(s) comprise administering to one or more cells that include one or more target genomic regions, a genome editing system and a DNA protein-kinase (DNAPK) inhibitor disclosed herein. Kits and compositions for editing a target gene comprise a genome editing system and a DNAPK inhibitor disclosed herein.
Owner:VERTEX PHARMA INC
Method of detecting protein charge variant and method for determining production process of bioproduct
A method of detecting a protein charge variant includes the steps of: analyzing to-be-detected protein via pH-IEC ion exchange chromatography to obtain a chromatogram map; on the basis of the chromatogram map, determining content of the to-be-detected protein charge variant. According to the embodiment, the method can detect the protein charge variant quickly, accurately and sensitively. The method especially is suitable for detection of the protein charge variant of recombinant DNA protein products, of which the pI value (isoelectric point) is 7.0-9.0, or a recombinant monoclonal antibody product.
Owner:HUBEI BIO PHARMA IND TECHCAL INST +1
Chromosome configuration acquisition library and construction method thereof
ActiveCN108265104AAvoid capturing inaccurate questionsImprove stabilityMicrobiological testing/measurementLibrary creationCross-linkSmall fragment
The invention discloses a chromosome configuration acquisition library and a construction method thereof. The construction method comprises preparing a single cell suspension from a tissue sample, carrying out cross-linking fixation on the single cell suspension to obtain a DNA-protein cross-linking complex, acquiring a DNA fragment from the DNA-protein cross-linking complex and constructing the chromosome configuration acquisition library. The tissue sample is processed into the single cell suspension and the single cell suspension is fixed through cross-linking so that the tissue cross-linking fixation effects are seriously affected by the tissue size, the excessive fixation of partial tissue cross-linking is avoided, a small fragment ratio is increased in the library and the interactioninformation between DNA and protein in the chromosome configuration is reduced. The method solves the problem that the cell morphology change causes low chromosome configuration acquisition accuracy,the stability and universality of the multi-tissue library are improved and the labor and reagent costs are greatly saved.
Owner:BEIJING NOVOGENE TECH CO LTD
Bacteriocin-producing paenibacillus and application thereof
ActiveCN110257269AIncrease catchIncrease conversion rateAntibacterial agentsCosmetic preparationsA-DNAAntibacterial activity
The invention provides a bacteriocin-producing paenibacillus ehimensis strain NPUST-1 which can promote the growth and immunoreaction of aquaculture organisms. At the same time, the strain can also improve the survival rate of aquaculture organisms after being infected with pathogenic bacteria. In addition, the bacteriocin Peocin produced by the paenibacillus ehimensis strain NPUST-1 is a DNA protective protein in the starvation / stagnation period, and has antibacterial activity against a variety of pathogens including culture pathogens, foodborne pathogens and clinical pathogens. The bacteriocin Peocin is found out to be the DNA protein in starvation / stagnation period and with antibacterial activity for the first time.
Owner:NAT PINGTUNG UNIV OF SCI & TECH
Promoter batch capture method
ActiveCN107091929AEfficient separationEfficient acquisitionMicrobiological testing/measurementBiological testingConserved sequenceTATA box
The invention provides a promoter batch capture method. According to the promoter batch capture method, when a plant gene is transcribed, a complex of a 'DNA-protein factor' formed in a promoter area is utilized, an improved chromatin immunoprecipitation technology is applied, and DNA containing an unknown plant gene promoter fragment is sorted out. The promoter batch capture method is a technology for specifically capturing an unknown plant gene promoter fragment by utilizing the property that protein factors of OsTBP2 and OsTFIIB are tightly combined with promotion and transcribe of TATA box short-chain conserved sequence which on the promoter and through the generation of a specific antibody of the two factors and through the combination of the chromatin immunoprecipitation technology (ChIP). In the technology, by utilizing the advantages that most promoters contain one TATA box short-chain conserved sequence, and through the combination of technology that the unknown plant gene promoter fragment is separated through the newly developed chromatin immunoprecipitation technology (ChIP), large-scale capture of the plant promoter is easily achieved, and the technology has high commercial value.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Anti-blain facial lotion
InactiveCN101327174AGood fading effectHigh activityCosmetic preparationsToilet preparationsWhelkThird generation
The invention belongs to the daily chemical technical field and relates to a beauty cosmetic processing technique formula-anti-acne beauty lotion. The anti-acne beauty lotion is characterized in that the beauty lotion is made from the raw materials with the following weight portions: 0.5g to 3g of Chinese rhubarb, 0.5g to 3g of forsythia, 0.5g to 3g of liquorice, 80g to 120g of glycerin, 650ml to 750ml of ethanol (95%) and the rest of purified water. The anti-acne beauty lotion of the invention is made from various Chinese medicines and other effective components to carry out the biological activity regulation for the skin according to the acne forming principle, can permeate to the dermis rapidly to promote the RNA and DNA protein synthesis and increase the vitality and the regeneration function of the skin through improving the skin microcirculation and regulating the cell metabolism, and then eliminate the sebum retention and the toxin which is generated by the propionibacterium which causes the acne so as to eliminate the sebum inflammation, thereby having favorable function for removing the acne; the whelk and the pox, and no scar is left after the acne is removed; so that the skin is bright, tender and moist and rich in elasticity, and the health is recovered.
Owner:刘艳
Ultrasonic crushing method for pig adipose tissue and application of ultrasonic crushing method
ActiveCN108949742AMaintain natural structureImprove cracking efficiencyMicrobiological testing/measurementMicroorganism lysisCross-linkSonification
The invention belongs to the field of co-immunoprecipitation chromatin of chromatins and particularly relates to an ultrasonic crushing method for a pig adipose tissue and application of the ultrasonic crushing method. The method comprises the following steps: (1) carrying out ice formaldehyde cross-linking reaction on the pig adipose tissue; (2) stopping cross-linking reaction through glycine, and then carrying out centrifugation and ice-bath layering; (3) acquiring a cell deposition layer of the pig adipose tissue; (4) extracting a cell nucleus deposition layer; and (5) ultrasonically breaking chromatins, so as to obtain small fragments of a protein-nucleic acid compound. The ultrasonic crushing method has the beneficial effects that a low-temperature condition is maintained in the wholeexperiment operation, so that a natural structure of a protein is guaranteed to a great extent, and the DNA-protein compound adsorbed with a real antibody is obtained; the obtained small fragments ofthe protein-nucleic acid compound can be directly applied to subsequent ChIP, the concentration of obtained DNA is determined, and the combination condition between DNA and a target protein in a whole genome range is detected by virtue of high-flux sequencing; and DNA obtained through enrichment is used for establishing a bank, is subjected to second-generation sequencing and is applied to the bioinformatics analysis of DNA of the pig adipose tissue.
Owner:WUHAN IGENEBOOK BIOTECH CO LTD
DNA-protein fusions and uses thereof
InactiveUS20080051299A1Easy to handleGood chemical stabilityBioreactor/fermenter combinationsPeptide librariesProtein neddylationDna protein
Owner:BRISTOL MYERS SQUIBB CO
DNase high-throughput sequencing detection signal processing method of DNA protein binding sites
InactiveCN104131093AImprove detection resolutionImprove recognition accuracyMicrobiological testing/measurementSequence analysisData setPretreatment method
The invention discloses a DNase high-throughput sequencing detection signal processing method of DNA protein binding sites. The method comprises the following steps: basic information of gene and DNase-Seq high-throughput sequencing detection data and ChIP-Seq high-throughput sequencing monitoring data of DNA protein binding sites are obtained; quality evaluation of the DNase-Seq high-throughput sequencing detection data is carried out, and credible sequencing data are screened out; only credible sequencing datum for directly reflecting sequencing initial position of protein binding sites is retained; a DNase-Seq detection sample data set is obtained; normalization processing is carried out on the DNase-Seq detection sample data set; the DNase-Seq detection sample data set is subdivided; and vertical summation of data in two subsets is carried out respectively from two directions of the front and back so as to finish operations. According to the invention, recognition precision and recognition resolution of the DNA protein binding sites are greatly raised.
Owner:HARBIN ENG UNIV
Yew anti-cancer paste
InactiveCN102824543AGrowth inhibitionHigh activityOrganic active ingredientsElectrotherapyActinidiaApoptosis
The invention provides a yew anti-cancer paste, which is an extract of more than ten rare traditional Chinese medicines including taxol, dragon's blood, oldenlandia diffusa and Chinese actinidia root. The external anti-cancer paste is prepared by ultrasonically crushing cytoplasm and coordinating with azone and magnetic chips. The paste has high drug loading capacity, drug factors of quantum particle peptides activate the magnetic memory of the drug factors due to the strong magnetic field function of the magnetic chips, the drug factors rapidly penetrate through the skin and enter the focus and penetrate through the cancer cells in a targeting mode, and the DNA protein chain polymerization is locked and is not depolymerized, so that the cancer cells atrophy, suicide and generate apoptosis. The paste is rapid in effect, high in air permeability, convenient and comfortable, has no toxic or side effect and constant-speed slow release, and has an extremely obvious treatment effect on cancers such as gastrointestinal carcinoma, lung cancer, breast cancer, skin cancer, nasopharynx cancer, laryngeal carcinoma, lymph gland cancer, superficial skin tumor and pancreatic cancer. The anti-cancer paste is simple in process, easy to operate, high in medicine activity and outstanding in antalgic anti-cancer effect.
Owner:林树芳
Whole genome interaction library and construction method thereof
ActiveCN108265049AGreat operabilityMicrobiological testing/measurementLibrary creationCross-linkEnzyme digestion
The invention provides a whole genome interaction library and a construction method thereof. The construction method comprises the following steps of step S1, using a primer sequence to link ends, generated by enzyme digestion, of a DNA-protein cross-linking complex to form a cyclic complex; step S2, conducting decrosslinking on the cyclic complex to obtain cyclic DNA; step S3, conducting fragmented library construction on the cyclic DNA to obtain the whole genome interaction library. The method uses a section of exogenous known DNA sequence to link the ends of enzyme-digested product fragments, so that the DNA-protein cross-linking complex forms the cyclic complex, then proteins in the cyclic complex are removed by crosslinking to obtain the cyclic DNA, the exogenous known DNA sequence carried by the cyclic DNA is used for fragmented library construction to obtain the whole genome interaction library for whole genome interaction research, and a whole genome interaction research methodwhich is simple and has wide operability is provided.
Owner:CHINA NAT RICE RES INST
Method for screening and evaluating genetic toxicity of industrial wastewater with complex ingredients
InactiveCN103134844AAccurate evaluationPromote degradationMicrobiological testing/measurementMaterial analysis by electric/magnetic meansCross-linkAdditive ingredient
The invention relates to a method for screening and evaluating genetic toxicity of industrial wastewater with complex ingredients. Gemmiparous broad bean seeds are cultivated in a suspension manner in to-be-detected wastewater solution prepared with tap water by means of gradient dilution; after two weeks, root tips are cut down and placed in proteinase K solution, and vacuumizing is carried out. Root tip cell nucleuses are separated, and the single-cell gel electrophoresis (SCGE) technique is used for separating DNA fragments. A fluorescence microscope is used for shooting comet pictures. A cometscore image-analysis system is used for measuring the tail length and the tail moment of the comet. Then, an SPASS program is used for statistic analysis. The method promotes proteinase K to penetrate into root tip meristem cell nucleuses by means of vacuumizing, accelerates degradation of DNA crosslinked protein, thus removes retardation of DNA-protein cross-linking on DNA fragments, and facilitates revelation and accurate evaluation of the true level of NDA breakage and damage inducted by the to-be-detected wastewater.
Owner:HUAINAN NORMAL UNIV
Method for extracting nucleic acid from bee pollen and purifying
InactiveCN103102378AWill not polluteHigh extraction rateSugar derivativesSugar derivatives preparationSolubilityIon exchange
The invention belongs to the technical field of biochemistry, and provides a method for extracting nucleic acid from bee pollen and purifying. The specific method is as follows: freezing screened, purified and dried bee pollen, and ultrafine grinding until the particle size achieves 20-60 meshes, adding a 0.14mol / L sodium chloride solution with weight ratio of 1: (8-10), homogenating, separating organelle through differential centrifugation, and ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain DNA-proteins, then adding a 1mol / L sodium chloride solution with weight ratio of 1: (6-8), homogenating, ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain RNA-nucleoproteins, adding a proper amount of sodium dodecyl sulfate to separate the proteins from nucleoproteins, adding concentrated potassium acetate, precipitating a sodium dodecyl sulfate-protein complex, transforming spare sodium dodecyl sulfate as sylvite with small solubility and simultaneously precipitating, repeatedly extracting, obtaining a nucleic acid pure solution through ultracentrifugation or ion-exchange column chromatography, and performing vacuum freezing and drying on the nucleic acid pure solution to obtain the pure nucleic acid substance.
Owner:大兴安岭绿源蜂业有限公司
Compound-type biological disinfector and preparation method thereof
InactiveCN108566958APowerful killStrong bactericidal specificityBiocideDisinfectantsDiseaseSide effect
Owner:FOSHAN WANYANG BIOLOGICAL TECH CO LTD
Method and kit for extracting genomic DNA from whole blood
InactiveCN109609493ASolve Z-value anomaliesSolve the abnormal Z value, a large number of fragments are missingDNA preparationBlood Collection TubeCross-link
The present invention provides a method and kit for extracting genomic DNA from whole blood. The method comprises: separating white blood cells from whole blood; breaking white blood cells to obtain broken white blood cells; and performing cell lysis and DNA-protein de-crosslinking on the broken white blood cells to obtain genomic DNA. By creatively breaking white blood cells and by breaking DNA and protein into small fragments, DNA-protein cross-linking sites are exposed to facilitate splitting and de-crosslinking, so that the extracted DNA is relatively more comprehensive and complete. The obtained DNA fragments satisfy the needs of subsequent database construction, and do not need to be broken again. Therefore, the method and kit specifically solves the problem that white blood cell fixation by preservation solution of a free DNA blood collection tube causes chromosome Z value abnormality, a large number of fragment deletions and repetitions.
Owner:BEIJING USCI MEDICAL LAB CO LTD
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