46 results about "Dna protein" patented technology

Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and/or germanium surfaces; a muL detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.

The invention provides a method of mapping DNA-protein interactions within a genome by fixing living cells to cross-link DNA and proteins, lysing the cells, and isolating chromatin by immunoprecipitation. DNA is purified and a SAGE protocol is performed on the purified DNA to produce GMAT-tag sequences, which are compared to a genomic sequence of the living cells to map DNA-protein interactions. The invention further provides a method of identifying an active chromatin domain and a method of identifying aberrant chromatin acetylation, wherein chromatin immunoprecipitation is performed using an antibody recognizing acetylated histone protein.

SPR-compatible substrates for high density microarray fabrication and analyses are provided. Novel carbon-on-metal thin film substrate architecture permits the integration of surface plasmon resonance detection with photolithographically fabricated biomolecule arrays for the analysis of biomolecular interactions. The utility of the technology is shown in the analysis of specific DNA-DNA, DNA-RNA and DNA-protein binding interactions. These new substrates may be used to determine the secondary structure of RNA molecules, to probe the sequence-specific binding kinetics and affinity of proteins and small molecules, and as substrates for small-molecule combinatorial chemistry platforms for drug discovery applications.

Methods are described for improvement of the serum half life of therapeutic nucleic acids by 3′ conjugation to useful target proteins, or other large molecules with useful function. In one embodiment, a 3′ A, C or G overhang is added to ds-DNA and the primary amines conjugated using biocompatible bifunctional linkers to proteins. The resulting nucleic acid-3′-conjugates are serum nuclease-resistant and retained in vivo for long periods without rapid kidney clearance. Further, the choice of conjugate imparts additional functionality to the nucleic acid-3-conjugate. For example, if the protein in the DNA-protein conjugate is the first component of the complement cascade (Clq or Clqrs) and the DNA aptamer has been developed against surface components of a target cell, it can be used to treat bacterial or parasitic infections and cancers. If the protein is serum albumin or another common (nonimmunogenic) blood protein and the aptamer is directed against a toxin or venom, the aptamer-protein conjugate can be used as an antidote that binds and neutralizes the toxin or venom. Similar DNA (aptamer)-nanotube, -enzyme, and -toxin conjugates could also be used to target and selectively kill bacteria, parasites, and cancer cells in vivo. If the protein is an Fc antibody fragment or C3b protein from the complement system and the aptamer is developed against a bacterial cell capsular material, other cell surface component or viral cell surface component, then the aptamer-3′-protein conjugate can aid in opsonization of the target cells or viruses by phagocytic leukocytes.

The invention relates to an ensemble learning method for predicting a DNA protein binding site. The method comprises the following steps of obtaining protein sequence data of the DNA protein binding site; preprocessing the protein sequence data of the DNA protein binding site; establishing input data in a one-hot coding mode; combining extracted features, establishing features of amino acid on each protein sequence, and taking the features as the input data; carrying out oversampling on positive sample data through utilization of an SMOTE algorithm; dividing negative sample data into a plurality of parts according to the positive sample quantity, and combining each part of negative samples with the positive samples to form a new data subset, thereby obtaining N data subsets; training eachdata subset through utilization of convolutional neural networks; and integrating results of N convolutional neural networks through utilization of a majority voting method, thereby obtaining a predicting result. According to the method, the DNA protein binding site predicting problem under the imbalance data condition is solved, and the predicting accuracy is improved.

The invention provides a bidirectional LSTM and CNN model for predicting DNA-protein binding. The model includes an input layer, a BLSTM layer, a convolutional layer, a maximum pooling layer, a full connection layer, and an output layer. Each input sequence is expressed as a four-line binary matrix by the input layer through single thermal coding. In the BLSTM layer, each LSTM model in a previouslayer will receive information of interest on DNA from an input sequence, and encode and interpret contributions from past historical information to a hidden state; then, the BLSTM module is propagated to the next BLSTM module, wherein a matrix scanned and input by each convolution kernel in the convolution layer is used for motif discovery, and information with different intensities is associatedwith potential sequence patterns; the maximum pooling layer is used for maximizing an output signal of each convolution kernel to form a complete sequence; the output layer performs non-linear conversion to determine DNA-protein binding feature information.

The invention discloses a method for extracting DNA from old formalin-fixed tissues. The basic principle of the method is to utilize the microwave thermal effect for fast removing formalin in the fixed tissues via water molecules, and eliminate the DNA-protein crosslinking caused by aldehyde groups of the formalin, thereby completely or partially recovering a DNA structure and further obtaining the DNA with better quality. The invention relates to the simple and high-efficient method for extracting the DNA from the old formalin-fixed tissues, which is based on microwave thermal remediation and can obtain the DNA with higher quality (large fragments and high purity), so that the method can be used for multiplex fluorescent PCR-capillary electrophoresis (FM-CE), denaturing high performance liquid chromatography (DHPLC) analysis, direct DNA sequencing and other molecular biological method analyses.

A method of detecting a protein charge variant includes the steps of: analyzing to-be-detected protein via pH-IEC ion exchange chromatography to obtain a chromatogram map; on the basis of the chromatogram map, determining content of the to-be-detected protein charge variant. According to the embodiment, the method can detect the protein charge variant quickly, accurately and sensitively. The method especially is suitable for detection of the protein charge variant of recombinant DNA protein products, of which the pI value (isoelectric point) is 7.0-9.0, or a recombinant monoclonal antibody product.

The invention discloses a chromosome configuration acquisition library and a construction method thereof. The construction method comprises preparing a single cell suspension from a tissue sample, carrying out cross-linking fixation on the single cell suspension to obtain a DNA-protein cross-linking complex, acquiring a DNA fragment from the DNA-protein cross-linking complex and constructing the chromosome configuration acquisition library. The tissue sample is processed into the single cell suspension and the single cell suspension is fixed through cross-linking so that the tissue cross-linking fixation effects are seriously affected by the tissue size, the excessive fixation of partial tissue cross-linking is avoided, a small fragment ratio is increased in the library and the interactioninformation between DNA and protein in the chromosome configuration is reduced. The method solves the problem that the cell morphology change causes low chromosome configuration acquisition accuracy,the stability and universality of the multi-tissue library are improved and the labor and reagent costs are greatly saved.

The invention provides a bacteriocin-producing paenibacillus ehimensis strain NPUST-1 which can promote the growth and immunoreaction of aquaculture organisms. At the same time, the strain can also improve the survival rate of aquaculture organisms after being infected with pathogenic bacteria. In addition, the bacteriocin Peocin produced by the paenibacillus ehimensis strain NPUST-1 is a DNA protective protein in the starvation/stagnation period, and has antibacterial activity against a variety of pathogens including culture pathogens, foodborne pathogens and clinical pathogens. The bacteriocin Peocin is found out to be the DNA protein in starvation/stagnation period and with antibacterial activity for the first time.

The invention provides a promoter batch capture method. According to the promoter batch capture method, when a plant gene is transcribed, a complex of a 'DNA-protein factor' formed in a promoter area is utilized, an improved chromatin immunoprecipitation technology is applied, and DNA containing an unknown plant gene promoter fragment is sorted out. The promoter batch capture method is a technology for specifically capturing an unknown plant gene promoter fragment by utilizing the property that protein factors of OsTBP2 and OsTFIIB are tightly combined with promotion and transcribe of TATA box short-chain conserved sequence which on the promoter and through the generation of a specific antibody of the two factors and through the combination of the chromatin immunoprecipitation technology (ChIP). In the technology, by utilizing the advantages that most promoters contain one TATA box short-chain conserved sequence, and through the combination of technology that the unknown plant gene promoter fragment is separated through the newly developed chromatin immunoprecipitation technology (ChIP), large-scale capture of the plant promoter is easily achieved, and the technology has high commercial value.

The invention belongs to the daily chemical technical field and relates to a beauty cosmetic processing technique formula-anti-acne beauty lotion. The anti-acne beauty lotion is characterized in that the beauty lotion is made from the raw materials with the following weight portions: 0.5g to 3g of Chinese rhubarb, 0.5g to 3g of forsythia, 0.5g to 3g of liquorice, 80g to 120g of glycerin, 650ml to 750ml of ethanol (95%) and the rest of purified water. The anti-acne beauty lotion of the invention is made from various Chinese medicines and other effective components to carry out the biological activity regulation for the skin according to the acne forming principle, can permeate to the dermis rapidly to promote the RNA and DNA protein synthesis and increase the vitality and the regeneration function of the skin through improving the skin microcirculation and regulating the cell metabolism, and then eliminate the sebum retention and the toxin which is generated by the propionibacterium which causes the acne so as to eliminate the sebum inflammation, thereby having favorable function for removing the acne; the whelk and the pox, and no scar is left after the acne is removed; so that the skin is bright, tender and moist and rich in elasticity, and the health is recovered.

The invention belongs to the field of co-immunoprecipitation chromatin of chromatins and particularly relates to an ultrasonic crushing method for a pig adipose tissue and application of the ultrasonic crushing method. The method comprises the following steps: (1) carrying out ice formaldehyde cross-linking reaction on the pig adipose tissue; (2) stopping cross-linking reaction through glycine, and then carrying out centrifugation and ice-bath layering; (3) acquiring a cell deposition layer of the pig adipose tissue; (4) extracting a cell nucleus deposition layer; and (5) ultrasonically breaking chromatins, so as to obtain small fragments of a protein-nucleic acid compound. The ultrasonic crushing method has the beneficial effects that a low-temperature condition is maintained in the wholeexperiment operation, so that a natural structure of a protein is guaranteed to a great extent, and the DNA-protein compound adsorbed with a real antibody is obtained; the obtained small fragments ofthe protein-nucleic acid compound can be directly applied to subsequent ChIP, the concentration of obtained DNA is determined, and the combination condition between DNA and a target protein in a whole genome range is detected by virtue of high-flux sequencing; and DNA obtained through enrichment is used for establishing a bank, is subjected to second-generation sequencing and is applied to the bioinformatics analysis of DNA of the pig adipose tissue.

The invention discloses a DNase high-throughput sequencing detection signal processing method of DNA protein binding sites. The method comprises the following steps: basic information of gene and DNase-Seq high-throughput sequencing detection data and ChIP-Seq high-throughput sequencing monitoring data of DNA protein binding sites are obtained; quality evaluation of the DNase-Seq high-throughput sequencing detection data is carried out, and credible sequencing data are screened out; only credible sequencing datum for directly reflecting sequencing initial position of protein binding sites is retained; a DNase-Seq detection sample data set is obtained; normalization processing is carried out on the DNase-Seq detection sample data set; the DNase-Seq detection sample data set is subdivided; and vertical summation of data in two subsets is carried out respectively from two directions of the front and back so as to finish operations. According to the invention, recognition precision and recognition resolution of the DNA protein binding sites are greatly raised.

The invention provides a yew anti-cancer paste, which is an extract of more than ten rare traditional Chinese medicines including taxol, dragon's blood, oldenlandia diffusa and Chinese actinidia root. The external anti-cancer paste is prepared by ultrasonically crushing cytoplasm and coordinating with azone and magnetic chips. The paste has high drug loading capacity, drug factors of quantum particle peptides activate the magnetic memory of the drug factors due to the strong magnetic field function of the magnetic chips, the drug factors rapidly penetrate through the skin and enter the focus and penetrate through the cancer cells in a targeting mode, and the DNA protein chain polymerization is locked and is not depolymerized, so that the cancer cells atrophy, suicide and generate apoptosis. The paste is rapid in effect, high in air permeability, convenient and comfortable, has no toxic or side effect and constant-speed slow release, and has an extremely obvious treatment effect on cancers such as gastrointestinal carcinoma, lung cancer, breast cancer, skin cancer, nasopharynx cancer, laryngeal carcinoma, lymph gland cancer, superficial skin tumor and pancreatic cancer. The anti-cancer paste is simple in process, easy to operate, high in medicine activity and outstanding in antalgic anti-cancer effect.

The invention provides a whole genome interaction library and a construction method thereof. The construction method comprises the following steps of step S1, using a primer sequence to link ends, generated by enzyme digestion, of a DNA-protein cross-linking complex to form a cyclic complex; step S2, conducting decrosslinking on the cyclic complex to obtain cyclic DNA; step S3, conducting fragmented library construction on the cyclic DNA to obtain the whole genome interaction library. The method uses a section of exogenous known DNA sequence to link the ends of enzyme-digested product fragments, so that the DNA-protein cross-linking complex forms the cyclic complex, then proteins in the cyclic complex are removed by crosslinking to obtain the cyclic DNA, the exogenous known DNA sequence carried by the cyclic DNA is used for fragmented library construction to obtain the whole genome interaction library for whole genome interaction research, and a whole genome interaction research methodwhich is simple and has wide operability is provided.

The invention relates to a method for screening and evaluating genetic toxicity of industrial wastewater with complex ingredients. Gemmiparous broad bean seeds are cultivated in a suspension manner in to-be-detected wastewater solution prepared with tap water by means of gradient dilution; after two weeks, root tips are cut down and placed in proteinase K solution, and vacuumizing is carried out. Root tip cell nucleuses are separated, and the single-cell gel electrophoresis (SCGE) technique is used for separating DNA fragments. A fluorescence microscope is used for shooting comet pictures. A cometscore image-analysis system is used for measuring the tail length and the tail moment of the comet. Then, an SPASS program is used for statistic analysis. The method promotes proteinase K to penetrate into root tip meristem cell nucleuses by means of vacuumizing, accelerates degradation of DNA crosslinked protein, thus removes retardation of DNA-protein cross-linking on DNA fragments, and facilitates revelation and accurate evaluation of the true level of NDA breakage and damage inducted by the to-be-detected wastewater.

The invention belongs to the technical field of biochemistry, and provides a method for extracting nucleic acid from bee pollen and purifying. The specific method is as follows: freezing screened, purified and dried bee pollen, and ultrafine grinding until the particle size achieves 20-60 meshes, adding a 0.14mol/L sodium chloride solution with weight ratio of 1: (8-10), homogenating, separating organelle through differential centrifugation, and ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain DNA-proteins, then adding a 1mol/L sodium chloride solution with weight ratio of 1: (6-8), homogenating, ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain RNA-nucleoproteins, adding a proper amount of sodium dodecyl sulfate to separate the proteins from nucleoproteins, adding concentrated potassium acetate, precipitating a sodium dodecyl sulfate-protein complex, transforming spare sodium dodecyl sulfate as sylvite with small solubility and simultaneously precipitating, repeatedly extracting, obtaining a nucleic acid pure solution through ultracentrifugation or ion-exchange column chromatography, and performing vacuum freezing and drying on the nucleic acid pure solution to obtain the pure nucleic acid substance.

The invention relates to a compound-type biological disinfector and a preparation method thereof, and belongs to the field of biotechnologies. The compound-type biological disinfector is capable of loading pharmaceutical active ingredient factors in multiple traditional Chinese medicinal materials on chitosan, compounding through subacidity electrolytic water, releasing to a space in a constant speed, performing a special reaction with an amino, and breaking activity of bacteria, penetrating a cytomembrane, breaking an organic matter chain structure, causing the generation of a DNA protein andthe synthesis retardation of a substance, strongly killing microorganisms, such as pathogenic bacteria and viruses, contained in the air, thereby effectively preventing and treating various airborneinfection diseases of influenza, respiratory tract infection, pneumonia and the like. Compared with the prior art, the compound-type biological disinfector is low in price, good in sterilizing effect,rapid in efficacy speed, extremely strong in killing capacity to the bacteria and the viruses, non-irritant, odorless, tasteless, low in toxic and side effects, non-irritant to skin and mucosa without a residual harmful substance, and has the important significance to improve the environment and guarantee the environmental sanitation.

The present invention provides a method and kit for extracting genomic DNA from whole blood. The method comprises: separating white blood cells from whole blood; breaking white blood cells to obtain broken white blood cells; and performing cell lysis and DNA-protein de-crosslinking on the broken white blood cells to obtain genomic DNA. By creatively breaking white blood cells and by breaking DNA and protein into small fragments, DNA-protein cross-linking sites are exposed to facilitate splitting and de-crosslinking, so that the extracted DNA is relatively more comprehensive and complete. The obtained DNA fragments satisfy the needs of subsequent database construction, and do not need to be broken again. Therefore, the method and kit specifically solves the problem that white blood cell fixation by preservation solution of a free DNA blood collection tube causes chromosome Z value abnormality, a large number of fragment deletions and repetitions.