DNase high-throughput sequencing detection signal processing method of DNA protein binding sites

Abstract

The invention discloses a DNase high-throughput sequencing detection signal processing method of DNA protein binding sites. The method comprises the following steps: basic information of gene and DNase-Seq high-throughput sequencing detection data and ChIP-Seq high-throughput sequencing monitoring data of DNA protein binding sites are obtained; quality evaluation of the DNase-Seq high-throughput sequencing detection data is carried out, and credible sequencing data are screened out; only credible sequencing datum for directly reflecting sequencing initial position of protein binding sites is retained; a DNase-Seq detection sample data set is obtained; normalization processing is carried out on the DNase-Seq detection sample data set; the DNase-Seq detection sample data set is subdivided; and vertical summation of data in two subsets is carried out respectively from two directions of the front and back so as to finish operations. According to the invention, recognition precision and recognition resolution of the DNA protein binding sites are greatly raised.

Description

technical field [0001] The invention belongs to a detection signal processing method of a DNA protein binding site, in particular to a DNase high-pass sequencing detection signal processing method of a DNA protein binding site. Background technique [0002] At present, the detection of DNA protein binding sites mainly adopts Chromatin Immunoprecipitation (ChIP). The ChIP-Seq technology, which combines the results of ChIP experiments with high-throughput sequencing technology, can efficiently detect DNA segments that bind to specific functional proteins on a genome-wide scale. The principle of ChIP-Seq is: firstly, the DNA fragments bound to the target protein are enriched by using the enzyme that specifically binds to the target protein through chromatin immunoprecipitation (ChIP), and then purified and library constructed. Then perform high-throughput sequencing on the enriched DNA fragments, and then accurately map the millions of read sequences obtained from the sequenci...

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Problems solved by technology

However, although the DNA protein binding site analysis method for ChIP-Seq detection data is very mature, this technology also has shortcomings. It cannot be detected without a suitable specific binding enzyme; secondly, only one protein can be detected in one experiment, which is time-consuming, labor-intensive, and expensive, and cannot be used on a large scale; The DNA fragment bound by the target protein is long, and only part of its two ends can be sequenced during sequencing. Since the sequencing region is not the binding site itself, although the resolution of the sequencing data can reach a single base, the binding site of the target protein The highest point positioning resolution can only reach tens of bases

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