3 results about "NUCLEIC ACID SUBSTANCE" patented technology

The present application relates to the technical fields of biological medicine and gene therapy, and particularly relates to a protonatable cationic lipid, a nucleic acid delivery system and application thereof. The protonatable cationic lipid has a structure as shown in formula (I), and contains a protonatable group such as a hydroxyl group or an amino group at the end. The protonatable cationic lipid can form a lipid nanoparticle for loading various nucleic acid substances. The lipid nanoparticle nucleic acid delivery system has high cell transfection efficiency in vitro, and can be targeted to organs such as liver in vivo to achieve efficient gene editing. The lipid nanoparticle constructed by the protonatable cationic lipid provided by the present application has high encapsulation efficiency, good stability and excellent delivery efficiency, and has a wide application prospect in the fields of gene therapy and cell engineering.

The present application relates to the field of biological medicine, and particularly relates to a kind of targeting CD19 nanobody and its preparation method and application.The present application provides a kind of nanobody targeting CD19, nucleic acid substance encoding the above-mentioned single domain antibody, corresponding expression vector and the host cell capable of expressing the nanobody targeting CD19.The nanobody provided by the present application has excellent specific CD19 binding capacity, and the nanobody affinity can reach 3.855x10 ‑9 ‌, show that the nanobody provided by the present application has highly specific binding activity, and has important application value in the treatment of B cell malignant tumor.

The application discloses collagen nanospheres and a preparation method and application thereof. The preparation method comprises the following steps: 0.01-2wt% of collagen, 0.1-8wt% of nucleic acid substances, 0.001-5wt% of positive electric substances, 0.1-7wt% of auxiliary agents and 78-99.78wt% of water are mixed according to the total mass of the raw materials as 100wt%; the nucleic acid substances are mixed with part of the water, and after high-pressure homogenization or microjet treatment, the nucleic acid semi-solid gel is obtained by low-temperature solidification, and the A phase is obtained by heating and melting; the collagen is dissolved in the A phase to obtain the B phase; the B phase and the C phase are mixed, and after high-pressure homogenization or microjet treatment, the auxiliary agent is added to form the collagen nanospheres; and the positive electric substances are dissolved in the residual water of the raw materials to obtain the C phase. The collagen nanospheres can improve the transmembrane transportation of the recombinant collagen and improve the bioavailability, and meet the application requirements of the collagen nanospheres in transdermal drug delivery products and the like.