47results about How to "Maintain natural structure" patented technology

The invention provides a new method for DNA sequencing called “natural sequencing by synthesis” (nSBS). According to the method, DNA that includes a desired sequence is synthesized using a dNTP mix with a small percentage of fluorescently-labeled nucleotides. The fluorescent label is cleavable. In contrast to previous methods that utilize 100% labeled nucleic acids, use of a small percentage of labeled nucleic acids minimizes the distortion of the natural structure of the extending DNA strand and the DNA polymerase. Using the disclosed methods with less than 10,000 copies of template DNA and 10% of the nucleotides labeled, long homopolymer stretches up to 20 bases can be sequenced with high accuracy and Q20 (with 99% accuracy) read lengths of up to 1,000 bases can be achieved. A Q20 read length of greater than 100 bases can potentially be achieved, even if the sequencing is performed with 1,000 copies of a template and 10% of the nucleotides labeled.

A ginkgolide compounds B with changed physical properties of ginkgolide B and high stability and water-solubility can be mixed with pharmacologically receptable carrier to obtain a composition used to treat ischemic apoplexy.

The invention discloses a porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering, which is implemented by adding anion biomacromolecules or cation biomacromolecules into a sufficiently wetted PADM water solution and regulating the electrokinetic potential (zeta potential) of collagen, wherein the anion biomacromolecules are sodium polyaspartate, the cation biomacromolecules are water-soluble chitosan, and the weight ratio of the biomacromolecules to the PADM is 1:5-1:25. The method disclosed by the invention effectively adjusts the pore size of the PADM, and adjusts the structure pattern of the collagen beam; and the obtained PADM has the natural structure of collagen, which predicts that the material can have wide application prospects in repair medicine.

The invention discloses a preparing method and application of bilobalide K with general formula as (I), which is characterized by the following: G is alkaline material; possessing stability and water-solubility.

The invention discloses an aseptic processing preparation method for allogeneic corneal grafts. By virtue of whole treatment on eyeballs, the method comprises the following steps: two-step virus inactivation, namely ultraviolet irradiation and sodium hypochlorite solution soaking, epithelial layer removal, two-step decellularization, namely serum soaking and an osmotic pressure method, cutting and dewatering, and glycerine solution storage. According to the preparation method disclosed by the invention, drying and terminal sterilizing steps are not needed, so that the preparation method is simple in process, short in production cycle and low in production cost; through soft decellularization treatment, the structure and components of the natural corneal stroma are kept to the maximal extent; no significant difference is formed among the transparency, the mechanical strength and the natural corneal stroma; the degradation speed is matched with the regeneration speed of newborn cornea tissues; antigen removal and virus inactivation are thorough; the biocompatibility and the biosecurity are high; the structure and performance of glycerine storage can be kept stable for a long period of time; clinical application is convenient; and corneal transplantation can be carried out instead of the allogeneic cornea.

The invention belongs to the field of chromatin immunoprecipitation, and specifically relates to a method for ultrasonically disrupting porcine adipose tissue and its application, including (1) carrying out formaldehyde cross-linking reaction on ice for porcine adipose tissue, (2) centrifuging after terminating cross-linking with glycine on ice And ice bath stratification, (3) obtaining porcine adipose tissue cell sedimentation layer, (4) obtaining cell nucleus precipitation layer, (5) ultrasonic disruption of chromatin to obtain small fragments of protein-nucleic acid complex five steps. The ultrasonic crushing method of porcine adipose tissue of the present invention maintains low temperature conditions throughout the experimental operation, which ensures the natural structure of the protein to a greater extent, thereby obtaining the DNA-protein complex adsorbed by the real antibody. And the obtained small fragments of the protein-nucleic acid complex can be directly used for subsequent ChIP, the concentration of the obtained DNA is detected, and the combination of the DNA and the target protein in the whole genome is detected by high-throughput sequencing. And further use the enriched dna to build a library and apply it to the bioinformatics analysis of porcine adipose tissue DNA after next-generation sequencing.

The invention discloses a compound of ginkgolides K of which a general formula is shown in (I), and a composition is prepared by mixing the ginkgolides K compound and pharmaceutically-acceptable medicine carrier, wherein G is alkaline substances. The invention also discloses a preparation method of the compound and treatment use thereof. In the invention, the ginkgolides K compound changes the physical property of the ginkgolides K, thereby the invention has the advantages of good stability, and the ginkgolides K compound has good water-solubility and higher stability. In the compound, a natural structure of the ginkgolides K can be kept, and when the compound is used as an effective component to enter the human body, the activity of the ginkgolides K can be fully developed to achieve theaim of treating an illness.