47results about How to "Maintain natural structure" patented technology

The invention provides a new method for DNA sequencing called “natural sequencing by synthesis” (nSBS). According to the method, DNA that includes a desired sequence is synthesized using a dNTP mix with a small percentage of fluorescently-labeled nucleotides. The fluorescent label is cleavable. In contrast to previous methods that utilize 100% labeled nucleic acids, use of a small percentage of labeled nucleic acids minimizes the distortion of the natural structure of the extending DNA strand and the DNA polymerase. Using the disclosed methods with less than 10,000 copies of template DNA and 10% of the nucleotides labeled, long homopolymer stretches up to 20 bases can be sequenced with high accuracy and Q20 (with 99% accuracy) read lengths of up to 1,000 bases can be achieved. A Q20 read length of greater than 100 bases can potentially be achieved, even if the sequencing is performed with 1,000 copies of a template and 10% of the nucleotides labeled.

The invention belongs to the technical field of bioengineering, and particularly relates to a production method for a yeast recombinant collagen, which includes construction of recombinant pichia pastoris engineered strain and production of constitutively and secretively expressed recombinant collagen without methanol induction. The invention first utilizes conventional codons of pichia pastoris at the genetic level to optimize a collagen gene sequence and performs artificial total gene synthesis, the optimized collagen gene sequence is then integrated into yeast chromosome, and thereby a constitutively and secretively expressed recombinant pichia pastoris engineered strain is constructed. The fermentation process is easy to operate, yield is high, and the separation and purification of the product are simple; methanol induction is not needed, the fire-proof and explosion-proof indexes of production equipment and factory buildings are low, and the production environment is environmentally friendly and pollution-free; and no toxic and harmful substances are use, so the product is safer. A specific affinity purification label is carried, the product with higher purity can be easily obtained, and the product can be applied to biomedical materials.

The invention discloses a preparing method and application of bilobalide K with general formula as (I), which is characterized by the following: G is alkaline material; possessing stability and water-solubility.

The invention relates to a novel gene sequence of a recombinant chicken Alpha interferon, constructs a recombinant expression vector thereof and belongs to a gene engineering biological product obtained by a molecular biology method. The gene sequence of a newly designed chicken Alpha interferon is recombined into a pPICZ Alpha-A vector and then is confirmed on the special position of a microzyme by an electric conversion mode. Besides, a pichia expression system is adopted to express a foreign gene, thus being beneficial to the commercial production of the chicken interferon. The protein expressed by a gene group after being diluted by 4365158.3 times can completely restrain the attraction of vesicular stomatitis virus of 100-1000TCID50. Compared with a natural chicken Alpha interferon, the novel gene sequence of a recombinant chicken Alpha interferon has a higher anti-virus effect; the anti-virus effect thereof is improved by 8 times. Test also detects that the protein expressed by the gene can restrain the proliferation of a newcastle disease virus and an avian influenza virus; besides, the effect of the interferon with high concentration is more remarkable.

The invention discloses an aseptic processing preparation method for allogeneic corneal grafts. By virtue of whole treatment on eyeballs, the method comprises the following steps: two-step virus inactivation, namely ultraviolet irradiation and sodium hypochlorite solution soaking, epithelial layer removal, two-step decellularization, namely serum soaking and an osmotic pressure method, cutting and dewatering, and glycerine solution storage. According to the preparation method disclosed by the invention, drying and terminal sterilizing steps are not needed, so that the preparation method is simple in process, short in production cycle and low in production cost; through soft decellularization treatment, the structure and components of the natural corneal stroma are kept to the maximal extent; no significant difference is formed among the transparency, the mechanical strength and the natural corneal stroma; the degradation speed is matched with the regeneration speed of newborn cornea tissues; antigen removal and virus inactivation are thorough; the biocompatibility and the biosecurity are high; the structure and performance of glycerine storage can be kept stable for a long period of time; clinical application is convenient; and corneal transplantation can be carried out instead of the allogeneic cornea.

A ginkgolide compounds B with changed physical properties of ginkgolide B and high stability and water-solubility can be mixed with pharmacologically receptable carrier to obtain a composition used to treat ischemic apoplexy.

The invention relates to an anti-contamination skin care composition and a cosmetic. The anti-contamination skin care composition comprises a substrate and the following components by weight percent:1.5-3% of chlorella extract, 2-6% of trehalose, and 1-3% of dimethyl methoxy chromanol. The anti-contamination skin care composition effectively forms an anti-contamination barrier for the skin, the tolerance of an irritant of the skin is improved, the PM2.5 of the skin is deeply removed, the oxidation resistance of the skin is improved, the cell and the DNA damage are repaired, and the anti-contamination skin care composition can resist the skin injury caused by ultraviolet light.

The invention relates to a recombinant expression plasmid of a dengue virus subunit vaccine. The recombinant expression plasmid contains a fused coding gene consisting the following elements: A) a signal peptide coding sequence; B) a dengue virus envelope glycoprotein EDIII coding region; and C) six continuous histidine coding sequences shown in SEQ ID NO:9. The vaccine is safe and new, has good immune effect and is suitable for industrialized production. The vaccine is fused with the protective antigen component of the dengue virus and can stimulate the organism to generate the immune response and be utilized to prevent the infectious diseases caused by the dengue virus.

The invention belongs to the field of co-immunoprecipitation chromatin of chromatins and particularly relates to an ultrasonic crushing method for a pig adipose tissue and application of the ultrasonic crushing method. The method comprises the following steps: (1) carrying out ice formaldehyde cross-linking reaction on the pig adipose tissue; (2) stopping cross-linking reaction through glycine, and then carrying out centrifugation and ice-bath layering; (3) acquiring a cell deposition layer of the pig adipose tissue; (4) extracting a cell nucleus deposition layer; and (5) ultrasonically breaking chromatins, so as to obtain small fragments of a protein-nucleic acid compound. The ultrasonic crushing method has the beneficial effects that a low-temperature condition is maintained in the wholeexperiment operation, so that a natural structure of a protein is guaranteed to a great extent, and the DNA-protein compound adsorbed with a real antibody is obtained; the obtained small fragments ofthe protein-nucleic acid compound can be directly applied to subsequent ChIP, the concentration of obtained DNA is determined, and the combination condition between DNA and a target protein in a whole genome range is detected by virtue of high-flux sequencing; and DNA obtained through enrichment is used for establishing a bank, is subjected to second-generation sequencing and is applied to the bioinformatics analysis of DNA of the pig adipose tissue.

The invention belongs to the field of food bio-utilization and relates to a method for extracting collagen from silver carp skins by an ultrasonic acid pretreatment assisted acid-enzyme method. The method comprises the following steps: reclaiming leftovers from a fish ball mill, picking out fish skins, performing washing, cutting-up and freeze-drying, sequentially soaking the fish skin in NaOH and isopropyl alcohol solutions, and removing protein, pigment and fat; and then, soaking the pretreated fish skins in lactic acid, performing ultrasonic acid-lactic acid pretreatment in an ultrasonic pond, adding pepsase into the mixed solution, performing acid-enzyme extraction, performing centrifuging to obtain a collagen crude extract; adding NaCl into the collagen crude extract to perform salting-out, performing centrifuging, dissolving the precipitate in an acetic acid solution, performing dialysis sequentially with an acetic acid solution and distilled water, and performing vacuum freeze drying to obtain collagen. The method mainly solves the problem resource waste of leftovers of fish product processing production, and overcomes the defect of low extraction rate of a traditional collagen extraction method; and the dialysis freeze-drying is represented in natural I-type collagen, and the extraction rate is 75.8 percent.

The invention provides hog cholera virus envelope protein oligomeric protein and a preparation method and application thereof. The oligomeric protein comprises hog cholera virus envelope protein molecules and exogenous oligomerization structural fragments, wherein the hog cholera virus envelope protein molecules are selected from hog cholera virus envelope protein E2 molecules and hog cholera virus envelope protein E0 molecules, the hog cholera virus envelope protein E2 molecules and the exogenous oligomerization structural fragments form the E2 oligomeric protein, and the hog cholera virus envelope protein E0 molecules and the exogenous oligomerization structural fragments form E0 oligomeric protein. The E2 oligomeric protein and the E0 oligomeric protein which serve as the vaccine antigens do not contain any genetic material RNA of hog cholera virus and are safe in production and use. The natural structure and bioactivity of envelope protein in the E2 oligomeric protein and the E0 oligomeric protein are kept, so that the E2 oligomeric protein and the E0 oligomeric protein which serve as the vaccine antigens are evident in immune effect. The oligomeric protein polymerizes the multiple subunit protein of the antigens, overcomes the shortcomings of subunit protein vaccines and is capable of evidently increasing vaccine immunogenicity, high in antigen content and easy in production and purification.

The invention relates to a dengue virus degeneration vaccine and application thereof. The amino acid sequence of the dengue virus degeneration vaccine is shown as SEQ ID NO.2; the amino acid sequence of the dengue virus degeneration vaccine contains an antigen peptide fragment, a dengue virus envelope glycoprotein EDIII region, a virus virulence related gene neutralizing epitope, a beta lamella and Loop amino acid, wherein the antigen peptide fragment is determined after sequence comparison analysis is carried out on a dengue virus representative strain which respectively contains serotypes 1,2,3,4; and the beta lamella retains a basic structure which forms the dengue virus envelope glycoprotein EDIII region. The dengue virus degeneration vaccine disclosed by the invention can be used for immunizing an organism, stimulating the organism to generate humoral immunity response and preventing the dengue virus infectious diseases.

The invention discloses an extraction method of collagen. The extraction method comprises the steps as follows: providing pretreated animal periderm to obtain a preform; soaking the preform by a fat treating agent, and cleaning the preform by alcohol with gradient concentration for degreasing treatment; digesting the preform after degreasing treatment in an enzyme digestion solution; and performing filtration, dissolution, salting out and dialysis on a mixture after digestive treatment to obtain the collagen. When animal periderm is used for collagen extraction, the method provided by the invention can increase the yield of collagen, and is easy to operate and low in cost.

The invention discloses a preparation method of hydrophobic wood, including the steps of: immersing the dried pretreated wood in a nano SiO2/polypropylene wax solution for hydrophobic treatment, infiltrating the nano SiO2/polypropylene wax melt into the wood, then hot pressing, densifying the surface of the wood, and finally removing the SiO2/polypropylene wax on the surface to obtain hydrophobicwood. The preparation method is simple, convenient in operation, short in preparation period, low in cost and good in environmental protection, surface hardness and mechanical properties of the hydrophobic wood are both improved, and the like.

The invention discloses a mulching film seed melon cultivation method for an arid desert region. The method comprises the steps of 1 land selecting and land preparing; 2 soil treating, wherein a nerium indicum leaf aqueous extract, a cephalotaxus fortunei stem and leaf aqueous extract and a derris root formaldehyde extract are mixed in proportion, diluted to 20 times with warm water at the temperature of 35 DEG C to 45 DEG C and then mixed with water to obtain 800-time liquid, soil is spray-irrigated with the 800-time liquid, after 2-3 days, diatomite, burned soil, biogas residues and tobacco leaves are mixed in proportion and then sprinkled to ridged ridge surfaces, and after a layer of straw powder is sprinkled, the ridge surfaces are covered with black film; 3 seed selecting and seed treating; 4 sowing; 5 field managing; 6 disease and pest preventing and controlling; 7 harvesting. The planting method is a simplified planting method integrating the functions of killing pests and bacteria, improving the soil structure, maintaining the microbial system balance and providing fertilizer moisture into a whole, the later-period managing cost is greatly reduced, the labor requirements are reduced, the economic benefit is increased, and a guarantee is provided for income increasing of farmers.

The invention discloses an assembled and detachable type in-situ test device for seepage of a fractured rock mass. The assembled and detachable type in-situ test device consists of a first fixed sealing device, a second fixed sealing device and a control system, wherein the first fixed sealing device and the second fixed sealing device are respectively and fixedly arranged in the rock mass, are respectively connected with the control system, and are respectively communicated with a fracture. The assembled and detachable type in-situ test device has the advantages that by performing the seepage test on the given fracture in the rock mass under different pressure conditions, the control system, the first fixed sealing device, the second fixed sealing device and the fracture form a closed loop, so as to automatically collect and store data; the structure is simple, and the assembly and detachment are convenient; the whole device is positioned in the original geological environment of the fractured rock mass, the natural structure and natural stress state of the fractured rock mass are maintained, and the hydraulic conduction coefficient of the fracture seepage is accurately obtained.