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Porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering

A technology of acellular dermis and tissue engineering, applied in the field of porcine acellular dermal matrix for tissue engineering, can solve the problems of destroying the natural structure of collagen, reducing biocompatibility and mechanical properties, and high extraction costs, and achieves wide application Prospects, important clinical application value and effect of social benefits

Inactive Publication Date: 2013-07-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional source of collagen is extracted from rat tail and tendons of cattle and horses. The extraction process not only destroys the natural structure of collagen, reduces its biocompatibility and mechanical properties, but also has high extraction costs.

Method used

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  • Porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering
  • Porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering
  • Porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of PADM natural scaffold with a pore size of 100-150 microns.

[0024] (1) Weigh a certain amount of PADM, then immerse it in ultrapure water 120 times its weight, and fully wet the scaffold for 15 hours in a constant temperature shaker at a speed of 80 rpm.

[0025] (2) Accurately weigh 7% chitosan by weight and dissolve it in the water containing the bracket, and continue to react for 12 hours in a constant temperature shaker at a speed of 120 rpm.

[0026] (3) Take out the bracket, wash with deionized water 3 times, 60 minutes / time, and the shaking table speed during washing is 80 rpm.

[0027] (4) Freeze at minus 70°C, and finally freeze-dry at minus 60°C for 16 hours.

[0028] The PADM obtained after the above steps has a pore diameter of 100-150 microns and a pore wall thickness of 5-10 microns. The tissue engineering PADM is composed of fiber bundles.

Embodiment 2

[0029] Example 2: Preparation of PADM natural scaffold with a pore size of 150-300 microns.

[0030] With embodiment 1, the quality of water-soluble chitosan is 20% of PADM weight in the step (2);

[0031] Washing time 5 times in step (3).

[0032] The PADM obtained after the above steps has a pore size of 150-300 microns and a tissue engineering PADM whose pore walls are composed of 5-10 micron loose collagen sheets.

Embodiment 3

[0033] Example 3: Preparation of PADM natural scaffold with a pore size of 50-100 microns.

[0034] With embodiment 1, water-soluble chitosan is changed into sodium polyaspartate;

[0035] The quality of sodium polyaspartate in step (2) is 8% of PADM weight;

[0036] The PADM scaffold obtained after the above steps has a pore size of 50-100 microns, and the pore wall is composed of sheet-like collagen with a thickness of 30-50 microns.

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Abstract

The invention discloses a porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering, which is implemented by adding anion biomacromolecules or cation biomacromolecules into a sufficiently wetted PADM water solution and regulating the electrokinetic potential (zeta potential) of collagen, wherein the anion biomacromolecules are sodium polyaspartate, the cation biomacromolecules are water-soluble chitosan, and the weight ratio of the biomacromolecules to the PADM is 1:5-1:25. The method disclosed by the invention effectively adjusts the pore size of the PADM, and adjusts the structure pattern of the collagen beam; and the obtained PADM has the natural structure of collagen, which predicts that the material can have wide application prospects in repair medicine.

Description

technical field [0001] The invention relates to a method for adjusting pores of porcine acellular dermal matrix (PADM) for tissue engineering, in particular to a method for regulating the pore diameter of porcine acellular dermal matrix (PADM) for tissue engineering with biological macromolecules, belonging to the field of biological materials. Background technique [0002] The research and development of skin and bone repair materials has always been the direction of a large number of scientists in the field of tissue engineering. Autologous transplantation has been clinically dominant. However, the source of autologous bone is limited, and allograft has the risk of immune rejection and infection. Various materials have been developed for use in the field of skin and bone tissue engineering. However, these materials have their own deficiencies and cannot fully meet the clinical requirements. Since the organic components in skin and bone are mainly composed of type I coll...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/56
Inventor 刘宏赵洪石王冠聪任娜陈丽梅李建华梁小萌刘铎江怀东陶绪堂王继扬
Owner SHANDONG UNIV
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