Extraction method of collagen

An extraction method and collagen technology, applied in the field of collagen extraction, can solve the problems of insufficient extraction of collagen, high cost of fetal skin, loss of collagen, etc.

Inactive Publication Date: 2019-12-13
成都奇璞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the extraction of collagen from fetal skin is easier than the pretreatment of adult animal fur, improper control in other steps will le

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The following steps are as follows to prepare a biomedical collagen material with unformed fetal cowhide as raw material:

[0055] (1) Raw material pretreatment

[0056] Remove the whole piece of fetal cowhide with unformed hair, stir and wash it with 0.4M / L NaCl for 2 hours, repeat the stirring and cleaning process twice, and then wash it twice with deionized water; put it in 0.1M / L NaOH and stir it for 4 hours, repeat Stir twice, wash with deionized water until neutral; add 4M / LNaCl+0.01M / LTris-HCL solution, stir and wash for 2 hours, wash with deionized water for 3 times; freeze at -80°C for 10-18 hours, take out and thaw , so repeated freezing and thawing for a total of 2 times. After the last thawing, gently scrape off the epidermis and subcutaneous fat, add 4M / L NaCl+0.01M / LTris-HCL solution again, stir and wash for 2 hours, and wash with deionized water 4 times. Spread it flat on a 2cm tray and store it at -80°C for 10-18 hours, then take it out and thaw it out....

Embodiment 2

[0071] Next, a biomedical collagen material is prepared from fetal pigskin with unformed hair, and the steps are as follows:

[0072] (1) Raw material pretreatment

[0073] Remove the whole piece of fetal cowhide with unformed hair, stir and wash it with 0.4M / L NaCl for 2 hours, repeat the stirring twice twice, and then wash it twice with deionized water; put it in 0.1M / L NaOH and stir it for 4 hours, repeat Stir twice, wash with deionized water until neutral; add 4M / LNaCl+0.01M / LTris-HCL solution, stir and wash for 2 hours, wash with deionized water for 3 times; freeze at -80°C for 10-18 hours, take out and thaw , so repeated freezing and thawing for a total of 2 times. After the last thawing, gently scrape off the epidermis and subcutaneous fat, add 4M / LNaCl+0.01M / L Tris-HCL solution again, stir and wash for 2 hours, and wash with deionized water 4 times. Spread it flat on a 2cm tray and store it at -20°C for 10-18 hours, then take it out and thaw it out. When it is not com...

Embodiment 3

[0087] Next, a biomedical collagen material is prepared from fetal pigskin with unformed hair, and the steps are as follows:

[0088] (1) Raw material pretreatment

[0089] Remove the whole piece of fetal cowhide with unformed hair, stir and wash it with 0.4M / L NaCl for 2 hours, repeat the stirring twice twice, and then wash it twice with deionized water; put it in 0.1M / L NaOH and stir it for 4 hours, repeat Stir twice, wash with deionized water until neutral; add 4M / LNaCl+0.01M / LTris-HCL solution, stir and wash for 2 hours, wash with deionized water for 3 times; freeze at -80°C for 10-18 hours, take out and thaw , so repeated freezing and thawing for a total of 2 times. After the last thawing, gently scrape off the epidermis and subcutaneous fat, add 4M / LNaCl+0.01M / L Tris-HCL solution again, stir and wash for 2 hours, and wash with deionized water 4 times. Spread it flat on a 2cm tray and store it at -20°C for 10-18 hours, then take it out and thaw it out. When it is not com...

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PUM

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Abstract

The invention discloses an extraction method of collagen. The extraction method comprises the steps as follows: providing pretreated animal periderm to obtain a preform; soaking the preform by a fat treating agent, and cleaning the preform by alcohol with gradient concentration for degreasing treatment; digesting the preform after degreasing treatment in an enzyme digestion solution; and performing filtration, dissolution, salting out and dialysis on a mixture after digestive treatment to obtain the collagen. When animal periderm is used for collagen extraction, the method provided by the invention can increase the yield of collagen, and is easy to operate and low in cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting collagen. Background technique [0002] Collagen is a polymer protein with triple superhelical structure synthesized by animal cells. It has good biological characteristics and is the main structural protein of connective tissue. In the dermis of animals such as cattle, pigs and sheep, about 70% is collagen. [0003] Collagen extracted and purified from animal dermis can be used as a tissue support to perform normal functions on cells, tissues and even organs, and has a good auxiliary effect on trauma repair. Compared with collagen extracted from animal dermis of other age groups, fetal skin (including fetal cowhide, fetal pig skin and fetal sheep skin, etc.) has the advantages of stable collagen, easy extraction, and high collagen content, so it is more suitable for cosmetic filling and cell scaffolding , Orthopedics, tissue damage repair, wound hemostasis ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/36C07K1/34C07K1/30
CPCC07K14/78
Inventor 岳崇霞宋磊
Owner 成都奇璞生物科技有限公司
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