The invention provides a collagen-based dura on the basis of a process-friendly type
ion liquid on-site
spinning technology, and a preparation method thereof. The preparation method comprises the steps of: dissolving I type collagen by a novel process-friendly
solvent system-
ion liquid and
sodium salt composite
system, fully soaking by taking decellularized pig
dermis as a substrate through an
electrolyte solution, carrying out electrostatic
spinning by taking air as a medium, and regulating the pore structure of collagen fibers by adjusting the
electrospinning conditions; removing residual
ion liquid with a proper eluent, and carrying out freeze-
drying, sterilization and packaging to obtain the collagen-based dura. The collagen-based dura has two or more
layers of structures: the aperture of collagen fibers at one side close to
brain tissue is controlled to be 3mu m and below, and the aperture of collagen fibers at one side far away from
brain tissue is controlled to be 20mu m and above. Compared with the traditional
solvent spinning, the preparation method is process-friendly, the toxic and side effects are reduced, and the triple-
helical structure of the collagen can be furthest retained.