Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture
A technology of zein and nanofiber membranes, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, fiber treatment, etc., can solve the problem of poor mechanical properties, difficult removal of chemical reagents, and effects on zein Fiber biocompatibility and other issues, to achieve the effect of improved mechanical strength, simple and effective preparation method, and good stability
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[0023] Example 1
[0024] Dissolve 1.5-4.0 grams of zein in a mixed solvent of 10ml of ethanol and water. The volume ratio of ethanol and water is 80:20, 70:30, 60:40 to make 15%-40% (w / v) The polymer solution is then added to a plastic syringe, the syringe is connected to a high-voltage positive electric field, the distance between the jet head and the receiver is controlled at 15cm~25cm, the solution flow rate is 0.5mL / h~2mL / h, the voltage is 10kV~18kV, and electrospinning , The strip-shaped structure nanofibers are obtained, and the nanofibers are randomly stacked to form a zein nanofiber membrane.
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[0025] Example 2
[0026] Dissolve zein in a mixed solvent of 10ml ethanol and water, the volume ratio of ethanol to water is 70:30, then add polyvinyl alcohol, the mass ratio of zein to polyvinyl alcohol is 5:1,4 :1,3∶1,2∶1,1∶1, magnetic stirring at 80℃ for 12h, prepare a polymer solution with a mass concentration of 15%, and then add it to a plastic syringe, the syringe is connected to a high-voltage positive electric field, the jet head and the receiver The distance between objects is controlled at 15cm~25cm, the solution flow rate is 0.5mL / h~2mL / h, and the voltage is 0kV~18kV. Electrospinning is performed to obtain a nanofiber membrane with greatly improved mechanical properties.
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