Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture

A technology of zein and nanofiber membranes, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, fiber treatment, etc., can solve the problem of poor mechanical properties, difficult removal of chemical reagents, and effects on zein Fiber biocompatibility and other issues, to achieve the effect of improved mechanical strength, simple and effective preparation method, and good stability
CN102587036AInactive Publication Date: 2012-07-18JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JILIN UNIV
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a corn alcohol-soluble protein nanofiber membrane used for cell culture. The corn alcohol-soluble protein / polyvinyl alcohol nanofiber membrane is a cell culture carrier material, which comprises biodegradable natural polymer corn alcohol-soluble protein and polyvinyl alcohol as a biocompatible high polymer. The transverse dimension of a ribbon-like corn alcohol-soluble protein / polyvinyl alcohol nanofiber is 625 to 3,920 nanometers; and the nanofibers are stacked into the nanofiber membrane randomly. The corn alcohol-soluble protein / polyvinyl alcohol nanofiber membrane is made through electrostatic spinning process, the preparation method is simple and effective, preparation equipment is simple, the stability is high, and indexes such as the diameters of the nanofibers and the porosity of a membrane material and the like can be regulated through changes of the condition of the electrostatic spinning process. The experiment proves that compared with a common PC (polycarbonate) plate, the corn alcohol-soluble protein nanofiber membrane material contributes to proliferation of mouse embryonic stem cells, and the diameter of the stem cell clone cultured for 5 days reaches 100 micrometers. Moreover, the nanofiber membrane can be recycled after being steamed and pressed for 30 minutes at 120 DEG C.
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Description

technical field

[0001] The invention relates to a method for preparing a nanofibrous membrane that can be used for cell culture. The biodegradable nanofiber can be used as a support material for cell culture, and belongs to the technical field of cell culture carrier materials. Background technique

[0002] In tissue engineering, cell carrier materials play the role of extracellular matrix, which is a bionic of the structure and function of extracellular matrix. The similarity in morphology and structure between the nanofibrous scaffold prepared by electrospinning and the extracellular matrix provides a source of motivation for its application in tissue engineering. The nanofibrous support material has high surface area and porosity, which facilitates cell adhesion and entry into the membrane material.

[0003] Zein is the main protein in corn gluten powder, which has good biocompatibility, biodegradability, film-forming properties and other properties. As a class of biode...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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