Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof

A liposome and artificial bone technology, applied in the field of medical materials, can solve the problems of poor osteoinductive ability, fast degradation and absorption, poor strength, etc., and achieve the effect of long usable time, high material strength and high mechanical strength

Active Publication Date: 2009-08-19
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, MIIG also has the following problems in the application process: (1) degradation and absorption are faster
(2) slightly weaker strength
(3) Osteoinductive ability is slightly poor
(4) The price is higher
As a classic drug

Method used

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  • Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof

Examples

Experimental program
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Example Embodiment

[0043] Example 1:

[0044] Weigh 15g of lecithin and 12g of cholesterol, dissolve it in 50ml of dichloromethane, shake it evenly to obtain solution I, then put it in an eggplant-shaped flask, distill under reduced pressure on a rotary evaporator, and occasionally introduce nitrogen until the dichloride Methane volatilizes completely, then vacuum drying at room temperature for 24h; weigh 3.0g bone morphogenetic protein (BMP) and dissolve it in 70ml PBS (pH=7.4) buffer solution and shake to obtain aqueous solution II; then add the above 70ml BMP solution II into the eggplant-shaped bottle Under the condition of ultrasound, the lipid film is fully hydrated. After ultrasound, the liposome containing BMP is obtained, which is freeze-dried as a powder for use.

[0045] Incubate calcium sulfate dihydrate at 100°C for 5 hours to generate calcium sulfate hemihydrate; mix 80g calcium sulfate hemihydrate, 10g calcium sulfate dihydrate and 10g α-tricalcium phosphate and grind in a ball mill f...

Example Embodiment

[0047] Example 2:

[0048] Weigh 15 g of carboxymethyl chitosan stearyl quaternary ammonium salt (weight average molecular weight of 2 million), dissolve 12 g of cholesterol in 50 ml of dichloromethane, and shake to obtain solution I; weigh 3.0 g of gentamicin solution Obtain the aqueous solution II in 70ml of deionized water; then blend and emulsify the above two solutions I and II, sonicate for 10 minutes, and put them into an eggplant-shaped bottle after forming a stable emulsion, and evaporate the methylene chloride on a rotary evaporator. Get gentamicin-containing polymer liposome aqueous solution, figure 1 It is a TEM picture of a polymer liposome containing gentamicin.

[0049] The calcium sulfate dihydrate was kept at 1200°C for 4 hours, and then quenched in an ice bath after being taken out to form anhydrous calcium sulfate. 100 g of anhydrous calcium sulfate was ground in a ball mill for 12 hours to obtain calcium sulfate powder.

[0050] The diluent is an aqueous soluti...

Example Embodiment

[0051] Example 3:

[0052] Weigh 15 g of carboxymethyl chitosan octadecyl quaternary ammonium salt (weight average molecular weight 1000), 12 g of cholesterol, and dissolve 2.0 g of carbon nanotubes in 50 ml of dichloromethane, and shake to obtain solution I; weigh 3.0 g BMP is dissolved in 70ml of deionized water to obtain aqueous solution II; then the above two solutions I and II are blended and emulsified, ultrasonicated for 10 minutes, after forming a stable emulsion, put into an eggplant-shaped bottle, and evaporate the dichloromethane on a rotary evaporator The liposomes containing carbon nanotubes and BMP are obtained cleanly, and the liposomes are lyophilized and used as powders for later use.

[0053] Incubate calcium sulfate dihydrate at 120°C for 8 hours to generate calcium sulfate hemihydrate, and heat calcium sulfate dihydrate at 1200°C for 2 hours to generate anhydrous calcium sulfate; combine 30g calcium sulfate dihydrate, 10g calcium sulfate hemihydrate, and 60g an...

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Abstract

The invention relates to a liposome calcium sulfate composite nanometer artificial boneware, as well as a preparation method and application thereof. The preparation method comprises the steps of well mixing liposome (1 to 40 percent), calcium sulfate powder (40 to 80 percent) and diluent (10 to 50 percent), curing the materials and then preparing a bone graft substitute containing nanometer liposome. The liposome can carry medicine and can modify surface, has two forms, namely solution and powder, and comprises traditional micromolecule liposome and macromolecule liposome having macromolecule characteristics. The invention can provide an artificial bone material which has nanometer reinforcing effects, function of sustaining and controlling medicine release, controllable degradation time, good biocompatibility and mechanical strength, can induce bone growth in vivo, and is beneficial to the healing of defective areas of bones.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a liposomal calcium sulfate composite nano-artificial bone, a preparation method and application. Background technique [0002] The key to the treatment of fractures near the joint is to ensure that after the fracture is reduced and fixed, the patient can perform functional exercises early to maximize the recovery of joint function. Bone grafting at the fractured bone defect can provide mechanical support and fill the gap at the same time, which accelerates the healing of the fracture and reduces the occurrence of complications such as loss of reduction, loose internal fixation, delayed union or even nonunion of the fracture. At present, there are many artificial bones used as bone graft substitutes. According to the composition, they can be divided into hydroxyapatite artificial bone, calcium phosphate artificial bone, and calcium sulfate artificial bone. When used, th...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/54
Inventor 段友容梁晓飞宋娜盛晶吴恒
Owner SHANGHAI INST OF ONCOLOGY
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