Collagen-based dura and preparation method thereof
A dura mater and collagen technology, applied in the field of collagen-based dura mater and its preparation, can solve the problems of difficult practical application requirements of dura mater substitutes, loss of collagen biological activity, unfriendly production process, etc., to achieve easy formation of large-scale industries Chain, sufficient source, low price effect
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[0043] Example 1:
[0044] (1) Preparation of collagen homogeneous electrospinning solution: first dissolve NaH2PO4 with a mass percentage of 0.1%wt in 1-allyl-3-methylimidazole benzoate ([BMIM][Ac] at room temperature and 25℃ ) To obtain an ionic liquid composite solvent system, gradually dissolve the cowhide type I collagen in the ionic liquid composite solvent system at 4°C, initially add 1% of the mass percentage of the ionic liquid composite solvent system, and then add the cowhide sequentially after it is fully dissolved Type I collagen, until the mass percentage of cowhide type I collagen in the ionic liquid composite solvent system is 5%, ultrasonic deaeration at 25°C until there are no bubbles;
[0045] (2) Preparation of conductive decellularized pig dermal substrate: cut fresh ecological pig skin into thin slices with a thickness of 0.5 to 1.0 mm, and ultrasonically treat it with peracetic acid with a mass concentration of 3.0% under ultrasound for 1 hour to inactivate t...
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[0050] Example 2:
[0051] (1) Preparation of collagen homogeneous electrospinning solution: first dissolve NaHSO4 with a mass percentage of 0.5%wt in 1-ethyl-3-methylimidazole glycinate ([EMIM][Gly]) at room temperature and 25°C to obtain Ionic liquid composite solvent system, gradually dissolve pig skin type I collagen in the ionic liquid composite solvent system at 10°C, initially add 3% of the mass percentage of the ionic liquid composite solvent system, and then add pig skin I sequentially after it is fully dissolved Type collagen, until the mass percentage of pig skin type I collagen in the ionic liquid composite solvent system is 10%, ultrasonic deaeration at 25°C until there is no air bubbles;
[0052] (2) Preparation of conductive decellularized porcine dermal substrate: the decellularized porcine dermis used was obtained in Example 1. The composition was 0.9%wt sodium chloride (NaCl), 0.01Mol / L phosphate buffer solution ( PBS), an electrolyte buffer solution with a vol...
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[0057] Example 3
[0058] (1) Preparation of collagen homogeneous electrospinning solution: first dissolve 1%wt NaHCO3 in 1-allyl-3-methylimidazole benzoate ([Amim][Ba] at room temperature and 25℃ ) To obtain an ionic liquid composite solvent system, and gradually dissolve the bovine Achilles tendon type I collagen in the ionic liquid composite solvent system at 20°C, initially add 5% of the mass percentage of the ionic liquid composite solvent system, and wait until it is fully dissolved. Add bovine Achilles tendon type I collagen until the mass percentage of bovine Achilles tendon type I collagen in the ionic liquid composite solvent system is 15%, and let it stand for defoaming at 25°C until there are no bubbles;
[0059] (2) Preparation of conductive decellularized porcine dermal substrate: The decellularized porcine dermis used was obtained from Example 1, and the composition was 0.9%wt sodium chloride (NaCl), 0.01Mol / L phosphate buffer solution ( PBS), an electrolyte buffer ...
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