1 results about "Natural stress" patented technology

The present application relates to the field of transcriptomics, and specifically discloses a method for identifying plant salt-tolerant germplasm resources by using multi-tissue transcriptome sequencing, comprising the following steps: S1, salt-tolerance evaluation and grade division: placing the plant germplasm to be tested under a suitable concentration of salt stress conditions for seedling culture, measuring its morphological indicators and physiological and biochemical indicators, calculating the salt-tolerance coefficients of each indicator, and using multivariate statistical analysis methods to comprehensively evaluate and grade the salt-tolerance of the germplasm; the present application first constructs a standardized salt-tolerance identification process from the laboratory to field application, uses gradient salt solution to simulate the natural stress environment, measures multi-dimensional indicators such as growth morphology, photosynthetic physiology and antioxidant system at the key growth stages, and uses statistical methods such as salt-tolerance coefficient, membership function and principal component analysis for comprehensive evaluation and grade division, thereby realizing systematic, rapid and accurate identification of the salt-tolerance of a large number of corn inbred lines.