Method for screening drought-resistant hemerocallis fulva based on hemerocallis fulva leaf temperature

A drought-resistant, Hemerocallis technology, applied in permeability/surface area analysis, removal of certain components for weighing, material inspection, etc., can solve problems such as land pollution, large human resources, and heavy workload.

Inactive Publication Date: 2020-05-15
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It requires a long measurement cycle, consumes a lot of human resources, and has a large workload. Large-scale use of chemical agents to measure the physiological state of plants requires high capital costs and can easily cause irreversible land pollution.

Method used

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  • Method for screening drought-resistant hemerocallis fulva based on hemerocallis fulva leaf temperature
  • Method for screening drought-resistant hemerocallis fulva based on hemerocallis fulva leaf temperature
  • Method for screening drought-resistant hemerocallis fulva based on hemerocallis fulva leaf temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for screening drought-tolerant daylilies based on daylily leaf surface temperature, comprising the following steps:

[0025] (1) The central console of the glass greenhouse displays the specific data of the experimental environment. The indoor temperature is 29.4 degrees Celsius, the indoor humidity is 95.2%, the carbon dioxide concentration is 1329PPM, and the light intensity is 15.7KL, keeping the experimental environment consistent.

[0026] Use a precision infrared thermometer to measure the leaf surface temperature of all varieties of Hemerocallis in the glass greenhouse. Select five Hemerocallis of the same variety with the same physiological state and similar varieties for measurement. The average temperature of three points on the leaf surface of each Hemerocallis is measured as a single plant Hemerocallis leaf surface temperature. Use excel software to screen and sort, and screen out the daylily varieties with the highest or lowest leaf surface tempera...

Embodiment 1

[0027] Example 1 is one of the Hemerocallis species with the highest leaf surface temperature: 'Mengzihua', and the measured leaf surface temperature is 32.6°C (one decimal point is reserved).

[0028] (2) Method for determining chromosomal ploidy of Hemerocallis cells: Take 10 mg of fresh and young Hemerocallis leaf tissue samples in a petri dish, add 400 μl of nuclear extraction buffer, chop the tissue with a sharp blade, and cut for 60-180 seconds to ensure that the Hemerocallis tissue Chopped evenly, add 1600μl DAPI staining solution and place at 20°C for 20min, use 38μm (400 mesh) to filter the sample, use the flow cytometer to measure, set the voltage to 400mV, adjust the threshold to the maximum, and set the flow rate to 0.4μl / s'Mengzihua 'Hemerocallis variety was measured as diploid.

[0029] (3) The method of measuring the stomatal conductance: under the natural light at 9-11 in the morning, select the sunny side of the leaf of Hemerocallis 'Mengzihua' variety to meas...

Embodiment 2

[0036] A method for screening drought-tolerant daylilies based on daylily leaf surface temperature, comprising the following steps:

[0037] (1) The central console of the glass greenhouse displays the specific data of the experimental environment. The indoor temperature is 29.4 degrees Celsius, the indoor humidity is 95.2%, the carbon dioxide concentration is 1329PPM, and the light intensity is 15.7KL, keeping the experimental environment consistent.

[0038] Use a precision infrared thermometer to measure the leaf surface temperature of all varieties of Hemerocallis in the glass greenhouse. Select five Hemerocallis of the same variety with the same physiological state and similar varieties for measurement. The average temperature of three points on the leaf surface of each Hemerocallis is measured as a single plant Hemerocallis leaf surface temperature. Use excel software to screen and sort, and screen out the daylily varieties with the highest or lowest leaf surface tempera...

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Abstract

The invention discloses a method for screening drought-resistant hemerocallis fulva based on hemerocallis fulva leaf temperature, which comprises the steps of S1, preliminary screening, specifically,measuring the leaf temperature of different hemerocallis fulva varieties, and sorting the leaf temperature of each hemerocallis fulva; S2, further determination, specifically, selecting the hemerocallis fulva with the highest or lowest leaf surface temperature in the hemerocallis fulva leaf temperature sequence in the step S1, measuring cell ploidy, stomatal conductance, leaf relative water content, detached leaf water loss rate and leaf pore size and density of the hemerocallis fulva, and confirming and screening drought-resistant hemerocallis fulva varieties according to survival ability ofthe hemerocallis fulva under a natural drought stress experiment and growth and development conditions after a rehydration experiment. The drought-resistant screening method provided by the inventionis simple and small in workload, avoids natural stress experiments of a lot of hemerocallis fulva, and only needs to perform temperature preliminary screening and then further perform confirmation experiments.

Description

technical field [0001] The invention belongs to the field of flower screening, and in particular relates to a method for screening drought-resistant daylilies based on the temperature of daylily leaves. Background technique [0002] Hemerocallis, known as Hemerocallis in ancient times, is a perennial herb, most of which are native to China, and the main varieties are collected by germplasm resource companies, botanical gardens and gardening enthusiasts in the United States from plants of the genus Hemerocallis produced in China and Japan, and are obtained by cross breeding, Hemerocallis It is easy to hybridize between species of genus plants, and the size, color, shape and flowering period of some species of flowers have a large range of changes, so they have long ornamental value, high ornamental value, various varieties, rich and gorgeous colors, and resistance to stress. As an ornamental plant, people pay more and more attention to it, and it has broad development prospec...

Claims

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Application Information

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IPC IPC(8): G01N25/20G01N15/14G01N5/00G01N5/04G01N23/2251G01N23/2202G01N15/08G01N21/84G01N33/00
CPCG01N25/20G01N15/14G01N5/00G01N5/045G01N23/2251G01N23/2202G01N15/088G01N21/84G01N33/0098
Inventor 李昊倪迪安梁锦钟荣谭雨馨
Owner SHANGHAI INST OF TECH
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