The invention relates to the non linear optical frequency conversion. To realize output of high power THz wave which can be continuously tuned, and stable running at room temperature, the technical scheme used by the invention is that: a tunable terahertz radiation source based on difference frequency cherenkov effect is composed of a laser device, a frequency doubling crystal, a double wavelength parametric oscillator, a harmonic mirror, a polarization filter, a combined beam mirror, a column lens and a difference frequency crystal; the harmonic mirror is placed between the frequency doubling crystal and the double wavelength parametric oscillator; the double wavelength parametric oscillator is II type phase matching KTP (Potassium Titanyl Phosphate) crystal OPO (Optical Parametric Oscillator); the polarization filter, the combined beam mirror and the column lens are arranged between the parametric oscillator and the difference frequency crystal; the difference frequency crystal is amagnesium oxide doped lithium niobate crystal with molecular formula of MgO:LiNbO3 or MgO:LN, and the generated THz wave is coupled and output by an Si prism on the side surface of the difference frequency crystal. The tunable terahertz radiation source based on difference frequency cherenkov effect is mainly applied to the optical frequency conversion.