The invention relates to a system and method for carrying out CARS (Coherent anti-Stokes Raman Scattering) imaging by using four-wave mixing signals generated by optical fiber. The system comprises a laser source, multiple reflecting mirrors, an optical parametric oscillator, a precise displacement platform, two long-wave-pass dichroscopes, a coupling objective lens, optical fiber, a two-dimensional scanner, a focusing lens, a supporting sleeve, a sample, a band-pass filter, an imaging lens, a detector and a computer. A light beam which is output after the laser source sends a frequency-doubling light beam for pumping the optical parametric oscillator, and a base-frequency light beam sent by the laser source are respectively pumping light and Stokes light for CARS imaging, and the four-wave mixing signals which are excited after the pumping light and the Stokes light are overlapped on time and space and then transmitted in the optical fiber are probe light for CARS imaging. CARS signal light which is generated after the pumping light and the Stokes light are focused on a sample are not overlapped with the frequency of the pumping light and the frequency of the Stokes light and can be separated by the long-wave-pass dichroscopes. Relative to the standard CARS system, the system disclosed by the invention is not newly provided with any device, has no special requirement on the used devices and is simplified in system and saved in cost.