System and method for carrying out CARS (Coherent anti-Stokes Raman Scattering) imaging by using four-wave mixing signals generated by optical fiber

Abstract

The invention relates to a system and method for carrying out CARS (Coherent anti-Stokes Raman Scattering) imaging by using four-wave mixing signals generated by optical fiber. The system comprises a laser source, multiple reflecting mirrors, an optical parametric oscillator, a precise displacement platform, two long-wave-pass dichroscopes, a coupling objective lens, optical fiber, a two-dimensional scanner, a focusing lens, a supporting sleeve, a sample, a band-pass filter, an imaging lens, a detector and a computer. A light beam which is output after the laser source sends a frequency-doubling light beam for pumping the optical parametric oscillator, and a base-frequency light beam sent by the laser source are respectively pumping light and Stokes light for CARS imaging, and the four-wave mixing signals which are excited after the pumping light and the Stokes light are overlapped on time and space and then transmitted in the optical fiber are probe light for CARS imaging. CARS signal light which is generated after the pumping light and the Stokes light are focused on a sample are not overlapped with the frequency of the pumping light and the frequency of the Stokes light and can be separated by the long-wave-pass dichroscopes. Relative to the standard CARS system, the system disclosed by the invention is not newly provided with any device, has no special requirement on the used devices and is simplified in system and saved in cost.

Description

technical field [0001] The invention relates to medical imaging technology and coherent anti-Stokes Raman scattering imaging technology, in particular to a method and system for coherent anti-Stokes Raman scattering imaging using four-wave mixing signals generated in optical fibers. Background technique [0002] Coherent anti-Stokes Raman scattering (CARS) technology is a new type of non-labeled molecular resonance imaging technology that has been developed rapidly since 1999. It is based on the Raman scattering resonance enhancement mechanism of chemical bonds. Imaging can be performed on specific chemical components in samples without any treatment, including chemical bonds such as C-H, O-P-O, Amide I, H-O (used for detection of liposomes, DNA, proteins, and hydrates respectively), enabling It has chemoselectivity, that is, specificity of imaging. Due to the coherent nature of CARS, its signal intensity is several orders of magnitude higher than that of the usual Raman sc...

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies of the background technology, the purpose of the present invention is to provide a method and system for eliminating the adverse effects of the FWM signal generated by the optical fiber on the imaging results, which is an inherent problem in the fiber-optic CARS imaging system

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