Preparation method of core-shell molecular imprinting nano-material, and application of nano-material

A molecular imprinting, core-shell technology, applied in chemical instruments and methods, ion exchange, other chemical processes, etc., can solve the problems of low adsorption capacity, enrichment capacity, and hindering applications, so as to facilitate entry and elution, Effect of Assay Increase, Increased Imprinting Sites, and Adsorption Efficiency

Inactive Publication Date: 2013-09-04
NANKAI UNIV
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Problems solved by technology

Our group once published molecularly imprinted resins with auxiliary recognition polymer chains (ARPCs) to purify natural trace protein BiP, see: Xia JJ, Long Y, Guo MJ, Wang Y, Mi HF. Science in China Series B: Chemistry, 2009 , 52:1388–93, but its low adsorption capacity and enrichment capacity hinder its further application

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  • Preparation method of core-shell molecular imprinting nano-material, and application of nano-material
  • Preparation method of core-shell molecular imprinting nano-material, and application of nano-material

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Embodiment

[0029] A method for preparing a core-shell type molecularly imprinted polymer nano silicon sphere, the steps are as follows:

[0030] 1) Synthesis of double bonded nano silicon spheres

[0031] Add 9 mL of tetraethoxysilane to 15 mL of absolute ethanol, 15 mL of distilled water and 3 mL of mixed solvent a with a concentration of 25% ammonia, and stir at 22°C for 24 hours. Then, 1 mL of γ-methacryloxypropyltrimethoxysilane was added to the above solution, and stirred at 22° C. for 48 hours. The resulting solution was centrifuged at 10,000 rpm for 10 minutes, the supernatant was decanted, the product was ultrasonically resuspended and washed with absolute ethanol, and then centrifuged again. Repeat the washing three times in this way, and place the centrifuged product in a vacuum oven to dry at room temperature for 48 hours to obtain double-bonded nano-silicon spheres;

[0032] 2) Synthesis of Assisted Recognition Polymer Chains (ARPCs)

[0033] Add monomer 0.5mL 4-vinylpyrid...

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Abstract

A preparation method of core-shell molecular imprinting nanometer silicon spheres comprises the following steps: synthesizing double bond-modified nanometer silicon spheres; synthesizing an auxiliary identification polymer chain with an identification group and an anchoring group which are randomly distributed; and self-assembling immunoglobulin heavy-chain binding protein BiP with the auxiliary identification polymer chain to prepare double-bond modified nanometer silicon spheres, and carrying out surface polymerization to form an imprinted polymer layer and an elution template. According to the invention, the prepared core-shell structured imprinting nanometer silicon spheres make imprinted sites be positioned on the material surface, substantially increase the imprinting specific surface area, and greatly increase the adsorption efficiency and adsorption quantity to target proteins; the existence of the auxiliary identification polymer chain makes imprinting have a specificity and a high efficiency, and the combination of the specificity and the high efficiency realizes the high-expansion and high-efficiency enrichment of natural microproteins and a strong practicality; and the use of cloned BiP as a template in the invention overcomes a problem that the natural microproteins cannot be obtained.

Description

technical field [0001] The invention relates to the technical field of synthesis and application of molecularly imprinted polymers, in particular to a method for preparing a core-shell molecularly imprinted nanomaterial and its application in the field of natural low-content protein purification. Background technique [0002] Molecularly imprinted polymers are artificial receptors formed by cross-linking and polymerizing functional monomers and cross-linking agents around template molecules. After the template molecules are extracted and eluted, the molecularly imprinted polymers leave a , spatial structure and binding site complementary cavities. Molecularly imprinted polymers can not only selectively recognize target molecules, but also have the advantages of simple synthesis, low cost, good physical strength, chemical stability, and reproducibility. Therefore, molecularly imprinted polymers have been widely used in analytical separation, solid phase extraction, chemical...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F230/08C08F226/06C08F220/44C08F220/56C08F222/38C08J9/26B01J20/26B01J20/30B01D15/08
Inventor 宓怀风刘德静王影靳素素
Owner NANKAI UNIV
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