53results about How to "Powerful kill" patented technology

The invention discloses an antimicrobial polycarbonate plastic and a preparation method thereof. The antimicrobial polycarbonate plastic comprises the following components in parts by weight: 100 parts of polycarbonate, 0.3-0.5 part of antimicrobial agent and 0.2-0.4 part of dispersant. The antimicrobial agent is one or mixture of nano titanium dioxide, nano silver oxide, polyhexamethyleneguanidine phosphate and N-(trichloromethylthio)phthalimide. The antimicrobial polycarbonate plastic disclosed by the invention can be easily processed into various shapes of interior ornaments of automobiles by an injection molding technique. The antimicrobial polycarbonate plastic disclosed by the invention has strong effects of bacterium control and killing and mold resistance.

The invention discloses an antimicrobial HIPS (high impact polystyrene) plastic and a preparation method thereof. The antimicrobial HIPS plastic comprises the following components in parts by weight: 100 parts of HIPS, 0.3-0.5 part of antimicrobial agent and 0.2-0.4 part of dispersant. The antimicrobial agent is one or mixture of nano copper oxide, nano silver oxide, polyhexamethyleneguanidine phosphate and N-(trichloromethylthio)phthalimide. The antimicrobial HIPS plastic disclosed by the invention can be easily processed into various shapes of interior ornaments of automobiles by an injection molding technique. The antimicrobial HIPS plastic disclosed by the invention can still has strong effects of bacterium control and killing and mold resistance after being used for a long time, and is free from the influences of illumination, temperature and other factors.

The invention discloses a double-target spot embedded antigen receptor, an encoding gene thereof, a plasmid containing gene, an immune T effect cell containing the gene and application of the gene. The embedded antigen receptor contains a CD4 antigen region (including a Leader region), a Linker region, a 17b single chain antibody region (17bscFv), a hinge region, a membrane spanning structural domain, a co-stimulation structural domain and a signal conduction structural domain. A binding region of gp120 is simulated by virtue of CD4-17bscFv-CAR, and the validity and safety of CD4-17bscFv-CAR are far higher than the validity and safety of a previously reported single-target spot CD4-zetaCAR.

The invention discloses an acarus-killing traditional Chinese medicine composition, as well as a preparation method and application thereof, and belongs to the field of daily-use chemicals. The acarus-killing traditional Chinese medicine composition is prepared from the following traditional Chinese medicine components in parts by weight: 10 to 35 parts of folium artemisiae argyi, 5 to 35 parts ofradix sophorae flavescentis, 5 to 30 parts of Sichuan pepper, 5 to 30 parts of fructus xanthii, 5 to 30 parts of sweet orange flower, 5 to 25 parts of fresh ginger, and 5 to 30 parts of tea seed cake. The traditional Chinese medicine composition provided by the invention can emit a special fragrance, so that acarids around a used part can be attracted, cranial nerves of the acarids are firstly anesthetized, then bran cells are stimulated until all nerves are interrupted, the acarids enter into a deep 'sleep' state so as to be prevented from mating and breeding, and the acarids are killed high-efficiently; and the traditional Chinese medicine composition has favorable efficacies on skin whitening and age defying, and has a favorable acarus-killing effect when being applied to daily-use chemicals.

The invention discloses a DC cell based on AFP antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of the DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by AFP antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, the DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses an ecological method for controlling apple snails in a rice field through tea seed cakes and a full-process two-section system for raising ducks in the rice field. The method comprises the following steps of spreading 45-55 kg, preferably 50kg, of tea seed cakes per mu in a period before planting and after plowing; using a nylon net with the height of 0.8-1.2 m, preferably1 m, for circumventing the rice field to provide convenience for breeding ducks and controlling the migration of apple snails and yellow weasels from the outside; 10 days after planting, putting the first batch of rice field ducks into the rice field on a sunny day and utilizing the ducks to swallow eggs of apple snails and young snails on ridges, weeds and rice plants, wherein large snails migrated into the rice field are eliminated by the ducks with the growth of the ducks; after heading of rice, removing the first batch of stocking ducks from the rice field to prevent the large ducks from damaging ears, wherein the second batch of ducks are required to be hatched at the end of tillering of the rice, and the second batch of ducks are put into the rice field after a heading stage to continue to swallow eggs of apple snails and small snails. The method not only can obviously reduce the snail control cost and increase the economic benefit, but also ensures that the ducks and the rice which are raised by the rice field are not contaminated by pesticide and herbicides and are safer.

The invention provides application of a covalent organic framework material to preparation of one or a plurality of tumor treatment medicine, photodynamic therapy medicaments and photothermal therapymedicaments. The covalent organic framework material is of a structure shown as a formula (I). The covalent organic framework material of the structure shown as the formula (I) is used for tumor treatment medicine, photodynamic therapy medicaments and photothermal therapy medicaments; after the optical excitation, the heat can be released, so that the photothermal therapy of the tumor is realized;the photodynamic and photothermal synergetic therapy can be realized under the single wavelength, so that the powerful cell killing is realized. In addition, the covalent organic framework material has an ERP effect; the enrichment of nanosheets in an in vivo experiment is improved; meanwhile, the photoacoustic imaging capability is realized; the optimal enrichment time of the covalent organic framework material can be determined, so that the tumor treatment under the imaging guidance is realized. The formula is shown in description.

The invention discloses an anion coating additive for removing formaldehyde and purifying air, and a preparation method and application of the anion coating additive. The anion coating additive for removing the formaldehyde and purifying the air is prepared from the following components in percentage by weight: 40-60% of base materials, 0.1-1% of a first dispersing agent, 0.1-1% of a first wettingagent, 20-35% of a first surfactant, 0.05-0.5% of a first defoaming agent, 0.1-1% of an anti-settling agent, 0.05-0.5% of a first preservative, 5-15% of a formaldehyde removing agent, and the balanceof water, wherein the base materials are prepared from the following components in percentage by weight: 20-35% of monazite powder, 30-45% of a compound containing rare earth, 0.1-1% of a second dispersing agent, 0.1-1% of a second wetting agent, 5-15% of a second surfactant, and the balance of water. The anion coating additive is scientific and reasonable in formula, zero VOC and zero formaldehyde are achieved, and heavy metal such as lead, cadmium, chromium and mercury is not contained; the removal rate of finished coating containing the coating additive to the formaldehyde in surrounding air can reach 95% or above, the finished coating has a long-acting removing effect on the formaldehyde, negative oxygen ions with high concentration, high vitality and small particle size can be released continuously, and the releasing number of anions is up to 9000 / cm<3>.

The invention discloses a DC cell based on SP17 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by SP17 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention relates to the field of vegetable cultivation, in particular to a fertilizer-free type melon and fruit cultivation organic medium. The organic medium is prepared from, by mass, 28-40% of coconut powder, 20-25% of mushroom dregs, 13-19% of oyster shell powder, 26-29% of cultivation padding, or 20-30% of coconut powder, 20-25% of mushroom dregs, 5-10% of oyster shell powder, 16-19% of tea fines and 26-29% of cultivation padding. The obtained organic medium has the advantages of being good in water retention capacity, abundant in nutrient, strong in disease resistance and the like.

The invention discloses a DC cell and a targeting immune cell population, and a preparation method and application thereof. The preparation method of DC cells includes the steps of: conducting first induced differentiation culture on mononuclear cells in order to obtain immature DC cells; transfecting the immature DC cells by using ck19 antigen mRNA, so as to obtain transfected immature DC cells; and conducting second induced differentiation culture on the transfected immature DC cells, so as to obtain mature DC cells, wherein the mononuclear cells are isolated from mononuclear cells of peripheral blood of a sample. The method can prepare DC cells safely and efficiently, has a low cost and high efficiency, and can effectively solve the problem of cytotoxicity. The method can be effectively used for immune cell treatment or stimulation of lymphocytes derived from a same patient to produce a targeting cell population containing a large amount of CTL cells, and then the cell population can be used for tumor immunotherapy to reach better effect.

The invention discloses a DC cell and a targeting immune cell population, and a preparation method and application thereof. The preparation method of DC cells includes the steps of: conducting first induced differentiation culture on mononuclear cells in order to obtain immature DC cells; transfecting the immature DC cells by using CEA antigen mRNA, so as to obtain transfected immature DC cells; and conducting second induced differentiation culture on the transfected immature DC cells, so as to obtain mature DC cells, wherein the mononuclear cells are isolated from mononuclear cells of peripheral blood of a sample. The method can prepare DC cells safely and efficiently, has a low cost and high efficiency, and can effectively solve the problem of cytotoxicity. The method can be effectively used for immune cell treatment or stimulation of lymphocytes derived from a same patient to produce a targeting cell population containing a large amount of CTL cells, and then the cell population can be used for tumor immunotherapy to reach better effect.

The invention relates to a traditional Chinese medicine composite for treating viral swine high fever, which is characterized by comprising the following components in parts by weight: 10-20 parts of astragalus extract, wherein astragalan is no less than 50 percent, UV; 10-20 parts of honeysuckle extract, wherein chlorogenic acid is no less than 5 percent, HPLC; 10-25 parts of hypericum perforatum extract, wherein hypericin is no less than 0.30 percent, UV; and 20-40 parts of rhubarb extract, wherein the total amount of total anthraquinone such as emodin, rhein, chrysophanol and the like is no less than 6 percent, HPLC. The traditional Chinese medicine composite is prepared by uniformly mixing the components in the proportion and finally subpackaging. The efficacies of all components of the traditional Chinese medicine composite generate the synergism to address both the symptoms and the root causes of the viral swine high fever, and therefore, the traditional Chinese medicine composite has the effects of clearing qi, letting off heat, cooling ying and detoxication, can strongly kill viruses, can also enhance the resistance of organisms, thereby efficiently treating diseases, such as hemorrhagic fever, epidemic parotitis, cheek influenza and the like, caused by viral infection fever, has enhanced and consolidated curative effect and can be produced with standardization and industrialization.

The invention relates to the field of cultivation matrixes, in particular to an organic matrix suitable for orchid cultivation. The organic matrix is prepared from, by mass, 50-60% of coconut shell L, 20-30% of tea fine powder and 10-20% of cultivation padding. The prepared organic matrix has the advantages of being high in disease resistance, high in water retention and ventilation performance and rich in nutrition.

The invention discloses an oil-control acne-removing acarus-killing face-cleaning facial cleanser containing deep sea salt and a preparation method thereof. The acarus-killing traditional Chinese medicine composition contained in the facial cleanser can emit a special fragrance with a strong acarus-killing effect. The contained acne-removing traditional Chinese medicine composition has the effects of controlling grease secretion, dredging pores, inhibiting bacteria, disinfecting and removing acnes. The traditional Chinese medicine composition has a synergistic effect, shows strong oil control, sterilization, acarus killing and acne removing effects, also has good effects of scavenging free radicals and inhibiting tyrosinase activity, and has certain anti-aging and whitening functions. The sea salt from dead sea has the effects of promoting blood circulation of a human body, sterilizing, relieving itching, relieving swelling and pain, whitening, removing cutin, acne and blackhead. In conclusion, through the synergistic interaction among the components, the oil-controlling, sterilizing, acarus-killing and acne-removing effects are powerfully achieved.

The invention discloses plant mite eliminating deep skin clearing face cream for controlling oil, removing small poxes and moisturizing and a preparation technology. A composition of plant extracts ofrosemary, dandelions, radix isatidis, isatis indigotica fortune, licorice roots, paris polyphylla and the like, contained in the face cream can emit special fragrance having efficacy of efficiently removing mites. Lipacide C8G ( Caprylylglycine ) contained in the face cream can deepen in pores and hair to clean dirt, and through cooperation of sodium shale oil sulfonat and plant extracts, the water-and-oil balance of skin can be efficiently adjusted, and obvious oil controlling efficacy is obtained. The composition of plant extracts, contained in the face cream has the efficacy of being efficiently nutritive and moisturizing, pure moistening and nourishing natural moisturizing force is provided for skin, and through cooperation of blue massaging granules, the skin environment can be awakened and moistened, nutrient absorption is facilitated, dryness, itch and discomfort symptoms of the skin can be relieved, and besides, the skin is full, and has elastic and healthy gloss. A white willow bark extract, a honeysuckle flower extract, a fructus forsythiae extracting solution, a white mulberry root bark extract and the like, which are contained in the face cream have the efficacy of efficiently resisting bacteria, dispelling small poxes, whitening and resisting ageing. The products can maintain week acid, and are mild and non-irritating.

The invention discloses a DC cell based on SPANXA1 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by SPANXA1 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a DC cell based on BCG1 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by BCG1 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention belongs to the field of cell biology, and particularly relates to an application of an antibacterial peptide in the in-vitro inhibition of Escherichia coli. The antibacterial peptide selected by the invention is a natural antibacterial peptide Esculentin-1A, and the amino acid sequence information of the antibacterial peptide Esculentin-1A is shown as SEQ ID NO.1. In the research process, it is found that the antibacterial peptide Esculentin-1A and citric acid are mixed according to a mass ratio of (7-10):(2-5), and in-vitro tests prove that the inhibition of the mixture on Escherichia coli reaches 99% or more. The antibacterial peptide and citric acid are mixed for use for the first time, and a new research and development direction is provided for the development of drugs related to the treatment of symptoms such as cholecystitis and ovaritis caused by Escherichia coli infection.

The invention discloses a DC cell based on HBV1 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of the DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by HBV1 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, the DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a DC cell based on PSA antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by PSA antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a DC cell based on P53 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by P53 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a preparation method of a modified nano-tourmaline powder / origanum oil extracting solution-rubber latex antibacterial film. The preparation method includes the steps: preparingmodified tourmaline powder and origanum oil extracting solution; performing reaction on natural rubber latex, the origanum oil extracting solution, vulcanizing ingredients and the modified tourmalinepowder to prepare modified nano-tourmaline powder / origanum oil extracting solution-natural rubber latex composite emulsion; finally, pouring the composite emulsion into a framed glass plate, drying the composite emulsion to form a film, placing the obtained dry film into a vacuum drying box, and performing additional vulcanization experiments to prepare the antibacterial film. The origanum oil extracting solution in the prepared antibacterial film contains 30 or more antibacterial compounds and can strongly kill most pathogenic bacteria, the antibacterial performance of the prepared compositerubber latex can be effectively improved when the origanum oil extracting solution is added into the natural rubber latex, and the added natural tourmaline powder can continuously release negative ions and far infrared rays for providing energy for a human body, can promote metabolism of the human body and has the healthcare functions of removing toxicity, balancing the pH (potential of hydrogen)value of the human body and the like.

The invention relates to the field of vegetable cultivation, in particular to a fertilization-free leaf vegetable cultivation organic medium. The fertilization-free leaf vegetable cultivation organic medium comprises, by weight, 25-35% of coconut powder, 30-37% of fungi residues, 18-20% of oyster shell powder and 15-20% of breeding padding; alternately, the fertilization-free leaf vegetable cultivation organic medium comprises, by weight, 15-20% of coconut powder, 29-34% of fungi residues, 18-20% of oyster shell powder, 10-15% of tea leaf fine powder, 15-20% of breeding padding and 1-3% of dried small shrimps. The fertilization-free leaf vegetable cultivation organic medium has the advantages of good water retention capacity, abundant nutrition, high disease resistance and the like.

A medicine for treating the onychomycosis is characterized by comprising the following raw materials by weight: 200-500 ml of mature vinegar, 20-200 purple garlic bulbs and 10-50 ml of concentrated iodine. A preparation method of the medicine comprises the following steps: putting the mature vinegar and the purple garlic bulbs into a mason jar, saving for 30 days in a sealing manner, and then opening and using vinegar liquor. The medicine has the benefits as follows: efficacies of each traditional Chinese medicine component in the medicine for treating the onychomycosis and the preparation method of the medicine are that the mature vinegar has an antibacterial and bactericidal efficacy, and can inhibit the growth and propagation of funguses; the garlic has a powerful sterilization effect; the concentrated iodine has a strong efficacy of killing pathogens; the medicine for treating the onychomycosis can enable protein of a pathogen to realize denaturation, and can kill funguses, bacterium and viruses, so as to treat the onychomycosis fundamentally. Compared with a traditional medicine for treating the onychomycosis, the medicine has the following advantages: the medicine provided by the invention is economical, practical, simple and convenient, and has an excellent curative effect; by using the medicine, a just infected patient with mild symptoms can recover after about 5 days, and a severe patient whose nails do not fall off totally can recover after about 1 month.

The invention provides a DC inducing and activating agent, which comprises small cell lung cancer specificity EGFR and aluminium adjuvant. The EGFR comprises many tumor cell surface structural clusters, and thus EGFR can be used as a simulated antigen to induce and stimulate DC during the culture process of DC so as to maintain the cell activity of DC in a high level. Moreover, an aluminum adjuvant is co-used with EGFR to sustain-release EGFR; the degradation and attenuation of EGFR is avoided, the aluminium adjuvant can interact with DC plasma membrane, and finally the antigen processing and presenting performances of DC are enhanced. The invention also provides a method using the provided DC inducing and activating agent to carry out DC in-vitro culture, DC obtained by the provided method, an application of DC in DC-CIK combined immunization, and CIK prepared through DC-CIK co-culture. Compared with the DC and CIK, which are prepared through a conventional method, the activity and proliferation performance of DC are improved; DC, which has been induced by EGFR, can represent more antigen information, and thus the obtained CIK has a higher activity and stronger performance on killing specific tumors.

The invention discloses a DC cell based on SURVIVIN antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by SURVIVIN antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a DC cell based on PAP antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by PAP antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a DC cell based on SCC antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by SCC antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.