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Promoter for expressing specificity of plant tissue and later development and application thereof

A plant tissue and promoter technology, applied in the biological field, can solve problems such as the burden on recipient plants, and achieve the effects of improving plant quality, reducing impact, and increasing concentration

Inactive Publication Date: 2010-09-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the CaMV35S promoter currently used in production is a constitutive promoter, and its continuous expression may cause a burden to recipient plants

Method used

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  • Promoter for expressing specificity of plant tissue and later development and application thereof
  • Promoter for expressing specificity of plant tissue and later development and application thereof
  • Promoter for expressing specificity of plant tissue and later development and application thereof

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Embodiment Construction

[0036] The experimental methods not specified in the following embodiments are conventional methods. The primer sequences used were synthesized by Shanghai Handsome Biotechnology Co., Ltd., and the endonucleases and polymerases used were all purchased from Bao Biological Engineering Co., Ltd. The biological materials used in this experiment include: Upland cotton (Gossypium.hirsutum) variety is Kezi cotton 312 (Udall J.A., Swanson J.M., Halle K., et al.A global assembly of cotton ESTs.Genome Res., 2006, (16 ):441-450), the Arabidopsis cultivars were Columbia type (Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR , Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, VanAken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC. Sequence and analysis of chr...

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Abstract

The invention relates to a promoter for the specificity expression and the later development expression of a plant tissue and application thereof, belonging to the biotechnology field. In the invention, a promoter sequence of a cotton cyclopropane fatty acid synthase with the length of 2.6kb is obtained. Place analysis proves that the promoter sequence contains a CAAT box, a TATA box, a cupric ion induced expression element, a pathogenicbacteria induced expression element, a dry response element, a cold stress response element, and the like as well as some root specificity expression elements and floral organ specificity elements. After GUS (beta-Glucuronidase) is dyed, a traditional gene is specially expressed at a later development stage by the promoter, and the expression parts are only restricted at a stem base part, a root and an anther.

Description

1. Technical field [0001] The invention relates to a plant gene promoter and its application, and provides a plant tissue-specific and growth-development later-stage specific promoter. Belongs to the field of biotechnology. It is used to improve plant quality and other beneficial production traits through plant genetic engineering technology. 2. Background technology [0002] The plant gene promoter is an important cis-acting element, which is a DNA sequence located in the upstream region of the 5' end of the structural gene, and is the center of transcriptional regulation. Since the first transgenic plant came out in 1983, promoters have always been a research hotspot in genetic engineering. Selecting a suitable and efficient promoter will lay a solid foundation for genetic engineering research. Constitutive promoters (such as the CaMV35S promoter) are often used in plant genetic engineering, but these constitutive promoters make their downstream genes continuously expres...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/63C12N1/00C12N1/21C12N15/82C12R1/19
Inventor 方先文杨郁文张保龙高媛媛
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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