5 results about "Tissue fluorescence" patented technology

The invention discloses a soft tissue thick slice preparation and high-resolution multicolor fluorescent three-dimensional imaging method. The method comprises the following steps: step 1, carrying out fixation and freezing protection treatment on a sample; step 2, putting the sample treated in the step 1 into an embedding medium, incubating and embedding to form an embedded block, and freezing and slicing the embedded block; the embedding medium comprises gelatin with a mass concentration of 8%, polyvinylpyrrolidone with a mass concentration of 2%, sucrose with a mass concentration of 20% and the balance of a phosphate buffer solution; step 3, unfreezing the slices in the step 2 in a room temperature environment, then carrying out pretreatment, antibody incubation and immunofluorescence staining, and carrying out pretreatment, antibody incubation and cell nucleus labeling in a dark environment; 4, performing three-dimensional imaging on the sample sliced in the step 3 by adopting a turntable confocal microscope to obtain three-dimensional data of tissue fluorescence distribution; however, the polyvinylpyrrolidone inhibits the formation and growth of ice crystals in the freezing process of the embedded block, and ensures the accuracy of the dyeing effect.

The invention discloses a tissue fluorescence motif with a controllable depth structure and a preparation method and application thereof, and belongs to the technical field of medical imaging and optical detection. The tissue fluorescence motif is prepared from a multi-component optical simulation material and a mold, and the material is prepared from the following raw materials in percentage: 0.01 to 0.05 percent of melanin solution, 0.01 to 0.1 percent of polystyrene microspheres, 0.03 to 0.08 percent of fluorescent agent, 1.0 to 1.5 percent of coagulator and the balance of liquid matrix. The tissue fluorescence motif has the beneficial effects that the tissue fluorescence motif can simultaneously simulate the absorption, scattering and endogenous fluorescence characteristics of tissues, so that the optical behavior of the tissue fluorescence motif is closer to that of real biological tissues. The design of the die body with controllable structured depth small holes enables exogenous fluorescence to be distributed at different depths according to requirements, so that depth gradient fluorescence signals in real tissues are simulated. And the preparation process is simple, high in repeatability, easy to standardize and capable of realizing batch production.

This invention relates to the field of optical imaging technology, specifically to a time-gated wide-field fluorescence lifetime imaging device and method, applicable to fluorescence lifetime imaging of biological tissues. The wide-field fluorescence lifetime imaging device includes an excitation module, a sample stage, a detection module, and a timing controller. The excitation module includes a laser, a homogenizing fiber, and a laser collimating lens; the sample stage is used to mount the imaging target; the detection module includes a filter switching assembly, a high-transmittance imaging lens, and an area array detector; the timing controller outputs a trigger signal to the laser to control the laser to emit pulsed laser light, and outputs a gating trigger signal to the area array detector to control the area array detector to open the gating exposure window at one or more preset delay time points after the laser stops emitting pulsed laser light. Thus, the area array detector and the timing controller can be controlled synchronously, thereby expanding the detection band, increasing imaging speed, and improving system stability in in vivo deep tissue fluorescence lifetime imaging.

The invention provides an amphiphilic aggregation-induced emission molecule as well as a preparation method and application thereof. Two nitrogen atoms in the amphiphilic aggregation-induced emission molecule provided by the invention are ionized, and the two nitrogen atoms are connected through a long alkyl chain, so that the molecule with two bromide ions is formed, and meanwhile, the molecule has cell membrane targeting property; one end with ions can be inserted into a phospholipid bilayer of a cell, so that stable cell membrane targeting capability is realized; in addition, the fluorescent probe also has a near-infrared first-area emission characteristic, so that the fluorescent probe can be used for nerve tracing and intraoperative nervous tissue fluorescence imaging.

Provided are covalent organic frameworks (COFs) and methods of making and using same. The COFs may be used in methods to visualize cancer cells and cancerous tissues at low temperatures associated with cryo-imaging and surgery. Visualization may be achieved via fluorescence. A COF of the present disclosure may have the following structure: formula (I)