47 results about "Tissue fluorescence" patented technology

Embodiments of the present invention provide an apparatus with a flexible probe suitable for determining properties of in vivo tissue from spectral information collected from various tissue sites. An illumination system provides light at a plurality of broadband ranges, which are communicated to a flexible optical probe. Light homogenizers and mode scramblers can be employed to improve the performance in some embodiments. The optical probe in some embodiments physically contacts the tissue, and in some embodiments does not physically contact the tissue. The optical probe receives light from the illumination system and transmits it to tissue, and receives light diffusely reflected in response to the broadband light, emitted from the in vivo tissue by fluorescence thereof in response to the broadband light, or a combination thereof. The optical probe can communicate the light to a spectrograph which produces a signal representative of the spectral properties of the light. An analysis system determines a property of the in vivo tissue from the spectral properties.

A method of determining a measure of a tissue state (e.g., glycation end-product or disease state) in an individual. A portion of the tissue of the individual is illuminated with excitation light, then light emitted by the tissue due to fluorescence of a chemical with the tissue responsive to the excitation light is detected. The detected light can be combined with a model relating fluorescence with a measure of tissue state to determine a tissue state. The invention can comprise single wavelength excitation light, scanning of excitation light (illuminating the tissue at a plurality of wavelengths), detection at a single wavelength, scanning of detection wavelengths (detecting emitted light at a plurality of wavelengths), and combinations thereof. The invention also can comprise correction techniques that reduce determination errors due to detection of light other than that from fluorescence of a chemical in the tissue. For example, the reflectance of the tissue can lead to errors if appropriate correction is not employed. The invention can also comprise a variety of models relating fluorescence to a measure of tissue state, including a variety of methods for generating such models. Other biologic information can be used in combination with the fluorescence properties to aid in the determination of a measure of tissue state. The invention also comprises apparatuses suitable for carrying out the method, including appropriate light sources, detectors, and models (for example, implemented on computers) used to relate detected fluorescence and a measure of tissue state.

A method of determining a measure of a tissue state (e.g., glycation end-product or disease state) in an individual is disclosed. A portion of the skin of the individual is illuminated with excitation light, then light emitted by the tissue due to fluorescence of a chemical with the tissue responsive to the excitation light is detected. The detected light can be combined with a model relating fluorescence with a measure of tissue state to determine a tissue state. The invention can illuminate the skin and detect responsive light over a time that spans a plurality of cardiac cycles of the individual, which can, as an example, help mitigate the effects of time-varying signals such as those due to hemoglobin. The invention can also determine the amount of light to be directed to the skin, for example by controlling the time that a light source is energized. The amount of illumination light can be determined from a skin reflectance characteristic such as pigmentation or melanin in the skin. Controlling the amount of light directed to the tissue can reduce the dynamic range required of a corresponding optical system, for example by allowing a single system to accurately measure individuals with very light skin and individuals with very dark skin.

A method of determining a measure of a tissue state (e.g., glycation end-product or disease state) in an individual. A portion of the tissue of the individual is illuminated with excitation light, then light emitted by the tissue due to fluorescence of a chemical with the tissue responsive to the excitation light is detected. The detected light can be combined with a model relating fluorescence with a measure of tissue state to determine a tissue state. The invention also can comprise correction techniques that reduce determination errors due to detection of light other than that from fluorescence of a chemical in the tissue (for example, the reflectance of the tissue). Other biologic information can be used in combination with the fluorescence properties to aid in the determination of a measure of tissue state.

Embodiments of the present invention provide an apparatus suitable for determining properties of in vivo tissue from spectral information collected from the tissue. An illumination system provides light at a plurality of broadband ranges, which are communicated to an optical probe. The optical probe receives light from the illumination system and transmits it to in vivo tissue and receives light diffusely reflected in response to the broadband light, emitted from the in vivo tissue by fluorescence thereof in response to the broadband light, or a combination thereof. The optical probe communicates the light to a spectrograph which produces a signal representative of the spectral properties of the light. An analysis system determines a property of the in vivo tissue from the spectral properties. A calibration device mounts such that it is periodically in optical communication with the optical probe.

The invention discloses an augmented reality near-infrared fluorescence navigation system and method. The system comprises a near-infrared fluorescence navigation device, which is used for exciting and capturing a fluorescence signal, converting the fluorescence signal into a fluorescence image, and processing the fluorescence image and then generating a projection image, and comprises an excitation light source, an optical filter group, an imaging lens group and a spectroscope; and a projection module, used for receiving the generated projection image and generating projection light accordingto the projection image, wherein the generated projection light passes through the spectroscope, and is imaged on an observation tissue after being focused by the imaging lens group. Through the projection technology, invisible near-infrared fluorescence is visualized, the reality enhancement effect is achieved, and the problem that traditional intraoperative navigation equipment needs to repeatedly observe a display image and check the position of an actual biological tissue fluorescence signal is solved.

The invention relates to a reference gene for fluorescence quantification of different tissues of Chinese yam and primers and application thereof, and belongs to the technical field of molecular biology of Chinese yam. The nucleotide sequence of the reference gene CKI-2 is as shown in SEQ ID NO. 1. The nucleotide sequence of a PCR amplification primer of the reference gene CKI-2 is as follows: anupstream primer is shown as SEQ ID NO.2; and the downstream primer is as shown in SEQ ID NO. 3. Protein kinase I, myb, F-box, actin gene, EF1, bHLH13, eIF1, grid heavy chain protein and other genes are selected as candidate genes of different tissues. The stability of candidate genes is evaluated through qRT-PCR in combination with RefFind, reference genes stably expressed in different tissues ofthe Chinese yams are selected out through screening, and a reference basis is provided for later development of research work such as gene function verification of growth and development and metabolicmechanisms of the Chinese yams.

The invention discloses a fluorescent microscope method for observing the growing situation of a plant pollen tube in a pistil with high efficiency and high definition. The method can be used for greatly weakening the phenomenon of pistil tissue fluorescence and reducing disturbance of the pistil in pollen tube observation when the pistil is amplified by a relatively large factor, so that a high-definition image of growth in a pollen tube at high amplified factor can be easily obtained, and details are ideally represented. According to the fluorescence microscope method, the softening transparent time is greatly shortened to several hours, the dyeing time is shortened from several hours in the prior art to several minutes, an observation experiment can be completed in one day, and the experiment efficiency is greatly improved; and application of a high-concentration NaOH solution is prevented, the material size can be kept in the original state in the softening transparent process, thephenomenon of water-loss shrinkage cannot happen, and re-watering treatment is not required.

Embodiments of the present invention provide an apparatus suitable for determining properties of in vivo tissue from spectral information collected from the tissue. An illumination system provides light at a plurality of broadband ranges, which are communicated to an optical probe. The optical probe receives light from the illumination system and transmits it to in vivo tissue, and receives light diffusely reflected in response to the broadband light, emitted from the in vivo tissue by fluorescence thereof in response to the broadband light, or a combination thereof. The optical probe communicates the light to a spectrograph which produces a signal representative of the spectral properties of the light. An analysis system determines a property of the in vivo tissue from the spectral properties. A calibration device mounts such that it is periodically in optical communication with the optical probe.