Reference genes for fluorescence quantitative detection of seven tissues of cherax quadricarinatus, screening method and application thereof

Abstract

The invention discloses reference genes for fluorescence quantitative detection of seven tissues of cherax quadricarinatus, screening method and application thereof, and belongs to the technical fieldof molecular biology. The invention comprises reference genes GAPDH and GST, which are used for fluorescence quantitative detection of seven tissues of cherax quadricarinatus; special primers for amplifying the reference genes, nucleotide sequence (shown as SEQ ID NO.1) of a forward primer F1 and nucleotide sequence (shown as SEQ ID NO.2) of a reverse primer R1 for amplifying the GAPDH gene, nucleotide sequence (shown as SEQ ID NO.3) of a forward primer F2 and nucleotide sequence (shown as SEQ ID NO.4) of a reverse primer R2 for amplifying the GST gene. According to the invention, appropriateand stable reference genes are screened out for research on gene expression of different tissues of the cherax quadricarinatus, which is conducive to accurately analyze the gene expression rule of the cherax quadricarinatus in experiments with different purposes, and provides a necessary molecular basis for subsequent research on the molecular mechanism of the cherax quadricarinatus.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to an internal reference gene for fluorescent quantitative detection of seven kinds of crayfish tissues, a screening method and application thereof. Background technique [0002] Internal reference genes are often used as references when detecting gene expression levels due to their stable expression in various tissues, different growth periods or developmental stages. The selection of internal reference genes can directly affect the accuracy of qRT-PCR results. Studies have shown that most internal reference genes are only stably expressed under specific tissue conditions and are not applicable to all species. Therefore, for different biological experiments, it is necessary to strictly screen the internal reference genes. Red claw crayfish (Cherax quadricarinatus), also known as four-ridge slip crayfish, is native to Australia and is also known as Australian ...

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Problems solved by technology

Studies have shown that most internal reference genes are only stably expressed under specific tissue conditions and are not applicable to all species

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