Quality control standard substance and quality control method for microorganism high-throughput DNA sequencing data

Abstract

The invention discloses a quality control standard substance and a quality control method for microorganism high-throughput DNA sequencing data. The quality control standard substance is an artificially synthesized polynucleotide sequence, the size of a nucleic acid molecule is 5Kb, the nucleic acid molecule is composed of five 1Kb fragments, the five 1Kb fragments can have A (5, 5) free combination modes, and different combination connection is carried out by adopting an overlap extension PCR molecular technology. According to the quality control method, the quality control standard substanceis utilized, and thus the quality control of factors such as laboratory management, personnel, detection experiments and bioinformatics analysis which influence the NGS detection result can be realized; meanwhile, errors caused by confusion of sample numbers in the sequencing process are prevented, and the test accuracy is improved; the quality control standard substance and a quality control method can be more effectively applied to various fields utilizing the high-throughput sequencing technology, the sequencing stability and accuracy can be effectively evaluated, and the sequencing data analysis reliability is ensured.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a quality control standard and a quality control method for high-throughput DNA sequencing data of microorganisms. Background technique [0002] High-throughput sequencing technology, also known as "next-generation sequencing (NGS) technology, can determine hundreds of thousands or even millions of sequences at one time, and is the most widely used sequencing technology today. Compared with the traditional Sanger sequencing technology, high-throughput sequencing has the advantages of high speed, high throughput, and low price. At present, high-throughput sequencing technology is widely used in many fields such as biological research, medicine, environment, agriculture, food safety, etc., and provides a very powerful tool for detecting the overall status of research at the molecular level. [0003] In addition to the complex technical process, high-throughput sequencing te...

AI Technical Summary

Problems solved by technology

This puts enormous pressure on the management, personnel training and quality control of NGS testing laboratories (including testing experiments and bioinformatics analysis).

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