mRNA and its preparation method and application

A DNA sequence and template technology, applied in DNA preparation, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of poor molecular uniformity, many by-product sequences, and low synthesis efficiency, and achieve strong stability and preparation High efficiency and the effect of improving production efficiency
CN109628442BActive Publication Date: 2021-09-28GENEO MEDICINE CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GENEO MEDICINE CO LTD
Publication Date
2021-09-28

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Abstract

The invention relates to the technical field of mRNA molecules, and specifically provides an mRNA and its preparation method and application. The preparation method of the mRNA comprises the following steps: providing a DNA transcription template; the DNA transcription template is composed of a sense strand and an antisense strand, wherein the sense strand comprises a promoter and a target DNA sequence connected to the promoter sequence, and the promoter The subsequence does not contain a stop codon, the target DNA sequence is identical to the target mRNA sequence, and the end of the target DNA sequence is connected with a polyadenylic acid sequence; under the action of RNA polymerase, the DNA transcription template is transcribed in vitro, Under the guidance of the promoter sequence, mRNA is transcribed. The preparation method of the present invention solves the problem of poor molecular homogeneity caused by the increase of by-product sequences during the Poly A tailing reaction of existing mRNA synthesis, and the preparation method also has the advantages of high preparation efficiency and easy purification of products.
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Description

technical field

[0001] The invention belongs to the technical field of mRNA molecules, and in particular relates to an mRNA and its preparation method and application. Background technique

[0002] With the development of nucleic acid technology, gene therapy has increasingly become a hot spot in the pharmaceutical industry. As an important gene therapy, mRNA drugs are increasingly favored by the market. The delivery of mRNA molecules into cells can produce various proteins, including soluble proteins, membrane proteins and intracellular proteins, especially proteins that can be produced by traditional pharmaceutical methods and obtained. Compared with DNA gene therapy, mRNA is a tool for transient expression, which has better biological safety and druggability. With the development and improvement of molecular biology, genetic engineering, and biochemistry, people can synthesize a large number of mRNA drug molecules in vitro, which brings great opportunities for gene ther...

Claims

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