Antibody detection method for combining three-chain DNA molecular beacons with nanopore technology for combination

A technology of DNA molecules and antibodies, which is applied in recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc. It can solve the problems of low signal characteristics, blocked signal unfavorable high-sensitivity detection, strong background interference, etc.
CN108467883AInactive Publication Date: 2018-08-31INST OF CHEM CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF CHEM CHINESE ACAD OF SCI
Publication Date
2018-08-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an antibody detection method for combining a three-chain DNA molecular beacon with a nanopore technology for combination. The method comprises the steps that the three-chain DNA molecular beacon used for detecting an antibody is designed and synthesized, when the antibody is combined with the three-chain DNA molecular beacon, the molecular beacon structure is destroyed, anda probe is released; an antibody-antibody capture DAN molecular complex cannot penetrate through a nanopore due to too large volume, the probe passes through the nanopore and can generate a characteristic current signal, the signal specificity is extremely high, molecular recognition can be accurately achieved, and the background interference problem is avoided. In addition, the unique thermodynamics and kinetic properties of the molecular beacon are combined with the nanopore single molecular technique, and high sensitivity detection on the variable antibody is achieved. In addition, the method has the advantages that implementation is easy, reagent consumption is extremely low, and large-scale equipment is not needed.
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Description

technical field

[0001] The invention relates to an antibody detection method combined with triple-strand DNA molecular beacon and nanopore technology. Background technique

[0002] Antibody is an important biochemical indicator, and its rapid and sensitive detection is not only of great significance for disease diagnosis, but also an important means of curative effect observation and disease prevention. At present, there are mainly two kinds of antibody detection techniques commonly used, enzyme-linked immunosorbent assay and Western blot. However, these two methods are not only complicated to operate and consume a large amount of reagents, but also the results obtained are usually semi-quantitative results. In recent years, with the rapid development of nanotechnology, various methods based on nanomaterials to detect antibodies with high sensitivity and selectivity have been developed. However, large instruments are still required and the process is cumbersome. Therefore...

Claims

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